Quantitative tracking of T cell clones after haematopoietic stem cell transplantation.
Bottom Line: Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood.Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation.We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.
Affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.Show MeSH
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Mentions: Clones, which survived, established a new balance that was unexpectedly stable, as revealed by in silico analysis, which demonstrates that the spectratypes and clonotypes skewed by HSCT remained intact over the next 6 months (Fig 2). Restoration of a full TCR beta repertoire which would re-produce the previous diversity and the clonal expansions normally present in healthy subjects, would probably take years, especially given the low abundance of naive T cells observed in the patient blood after HSCT (Supporting Information Fig 8).
Affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.