Quantitative tracking of T cell clones after haematopoietic stem cell transplantation.
Bottom Line: Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood.Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation.We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.
Affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.Show MeSH
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Mentions: HSCT decreased overall diversity of T cell clones (Supporting Information Fig 6), while the number of ‘hyper-expanded’ clones (comprising >1% of all TCR beta sequences) increased, leading to the propagation of specific TCR V beta gene families (Supporting Information Fig 7). The cumulative contribution of ‘hyper-expanded’ clones increased from 3% before to 26% after HSCT and remained at this high level for at least 10 months after HSCT (Fig 1A).
Affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.