Disruption of the SapM locus in Mycobacterium bovis BCG improves its protective efficacy as a vaccine against M. tuberculosis.
Bottom Line: Both systemic and intratracheal challenge of mice with Mycobacterium tuberculosis following vaccination showed that the SapM mutant, compared to the parental BCG vaccine, provided better protection: it led to longer-term survival.Persistence of the SapM-mutated BCG in vivo resembled that of the parental BCG indicating that this mutation will likely not compromise the safety of the BCG vaccine.The SapM mutant BCG vaccine was more effective than the parental vaccine in inducing recruitment and activation of CD11c(+) MHC-II(int) CD40(int) dendritic cells (DCs) to the draining lymph nodes.
Affiliation: Unit for Medical Biotechnology, Department for Molecular Biomedical Research, Ghent, Belgium. email@example.comShow MeSH
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Mentions: Similarly, we analysed the uptake of the parental M. bovis BCG and the SapM mutant by bone marrow-derived dendritic cells (BM-DCs). Again, WT M. bovis BCG and the SapM mutant were taken up by murine DCs with the same efficiency (Fig 3A).
Affiliation: Unit for Medical Biotechnology, Department for Molecular Biomedical Research, Ghent, Belgium. firstname.lastname@example.org