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Ultrastructural Changes in Rat's Atrial Cardiomyocytes After Short Term Administration of Amiodarone and Possible Protective Role of Vitamin E.

Shatoor AS, Zaki MS, Eid RA, Sayed-Ahmad MA - J Clin Med Res (2012)

Bottom Line: The use of amiodarone is associated with ultrastructural changes affecting body tissues, but its effect on the ultrastructure of the heart has not yet been fully elucidated.The aim of this study is to test the adverse effects of amiodarone administration on cardiomyocytes and to study the possible protective role of vitamin E co-administration.A total of 18 adult male albino rats were used in this study.

View Article: PubMed Central - PubMed

Affiliation: Internal Medicine Department, Cardiology Section, Faculty of Medicine, King Khalid University, Saudi Arabia.

ABSTRACT

Background: Amiodarone chlorhydrate is a diiodated benzofuran derivative used to treat a variety of cardiac rhythm abnormalities. The use of amiodarone is associated with ultrastructural changes affecting body tissues, but its effect on the ultrastructure of the heart has not yet been fully elucidated.

Methods: The aim of this study is to test the adverse effects of amiodarone administration on cardiomyocytes and to study the possible protective role of vitamin E co-administration. A total of 18 adult male albino rats were used in this study. The rats were divided randomly into three groups of 6 rats each as follows: group I was considered the control group and was given vegetable oil; group II received 54 mg/kg of oral amiodarone; and group III received a single dose of combined vitamin E (50 mg/kg) and amiodarone (54 mg/kg). After 2 weeks, the rats were sacrificed, and the atrial tissues were harvested and processed for electron microscopic study.

Results: ADMINISTRATION OF AMIODARONE ALONE MODIFIED THE ATRIAL ARCHITECTURE, WHICH WAS DEMONSTRATED BY THE FOLLOWING: mitochondrial enlargement and cristae lysis; marked heterogeneity of myofibril patterns with partial necrosis and disintegration of myofilaments; and irregularities of the sarcomere and less concentration of atrionatriuretic factor (ANF) granules, which localised in closed proximity to the nucleus with disrupted chromatin contents. Concomitant administration of vitamin E with amiodarone showed a considerable preservation of the atrial architecture.

Conclusions: Oral administration of amiodarone in rats resulted in ultrastructural changes in atria, which can be attenuated by vitamin E co-administration.

No MeSH data available.


Related in: MedlinePlus

Electron micrographs from cardiac atria of amiodarone- and vitamin E-treated rats. A. A semithin section of cardiac muscle showing sarcoplasm of a cardiomyocyte occupied by normal myofibril striations (m), and other myofibrils (stars) appear disrupted. Dissociation of myofibrils in some (stars) and normal pattern (m) in others. B. ANF granules is still less in size (An) while the mitochondria (M) were preserved with its cristae (Bar = 1 µm). (x: 1000).
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Figure 3: Electron micrographs from cardiac atria of amiodarone- and vitamin E-treated rats. A. A semithin section of cardiac muscle showing sarcoplasm of a cardiomyocyte occupied by normal myofibril striations (m), and other myofibrils (stars) appear disrupted. Dissociation of myofibrils in some (stars) and normal pattern (m) in others. B. ANF granules is still less in size (An) while the mitochondria (M) were preserved with its cristae (Bar = 1 µm). (x: 1000).

Mentions: Concomitant administration of vitamin E with amiodarone showed a considerable protection of the atrial tissues. Microscopic study of the atrial tissue of this group showed that the sarcoplasm was occupied by normal myofibrils with clear striations and others had been disrupted. These changes did not demonstrate marked cardiac damage as in the amiodarone-treated group, although occasional focal loss of some myofibrils was observed (Fig. 3A, 3B). There were no alterations in mitochondrial morphology compared to the second group (preserved cristae, normal size and shape) (Fig. 3B).


Ultrastructural Changes in Rat's Atrial Cardiomyocytes After Short Term Administration of Amiodarone and Possible Protective Role of Vitamin E.

Shatoor AS, Zaki MS, Eid RA, Sayed-Ahmad MA - J Clin Med Res (2012)

Electron micrographs from cardiac atria of amiodarone- and vitamin E-treated rats. A. A semithin section of cardiac muscle showing sarcoplasm of a cardiomyocyte occupied by normal myofibril striations (m), and other myofibrils (stars) appear disrupted. Dissociation of myofibrils in some (stars) and normal pattern (m) in others. B. ANF granules is still less in size (An) while the mitochondria (M) were preserved with its cristae (Bar = 1 µm). (x: 1000).
© Copyright Policy - open access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376877&req=5

Figure 3: Electron micrographs from cardiac atria of amiodarone- and vitamin E-treated rats. A. A semithin section of cardiac muscle showing sarcoplasm of a cardiomyocyte occupied by normal myofibril striations (m), and other myofibrils (stars) appear disrupted. Dissociation of myofibrils in some (stars) and normal pattern (m) in others. B. ANF granules is still less in size (An) while the mitochondria (M) were preserved with its cristae (Bar = 1 µm). (x: 1000).
Mentions: Concomitant administration of vitamin E with amiodarone showed a considerable protection of the atrial tissues. Microscopic study of the atrial tissue of this group showed that the sarcoplasm was occupied by normal myofibrils with clear striations and others had been disrupted. These changes did not demonstrate marked cardiac damage as in the amiodarone-treated group, although occasional focal loss of some myofibrils was observed (Fig. 3A, 3B). There were no alterations in mitochondrial morphology compared to the second group (preserved cristae, normal size and shape) (Fig. 3B).

Bottom Line: The use of amiodarone is associated with ultrastructural changes affecting body tissues, but its effect on the ultrastructure of the heart has not yet been fully elucidated.The aim of this study is to test the adverse effects of amiodarone administration on cardiomyocytes and to study the possible protective role of vitamin E co-administration.A total of 18 adult male albino rats were used in this study.

View Article: PubMed Central - PubMed

Affiliation: Internal Medicine Department, Cardiology Section, Faculty of Medicine, King Khalid University, Saudi Arabia.

ABSTRACT

Background: Amiodarone chlorhydrate is a diiodated benzofuran derivative used to treat a variety of cardiac rhythm abnormalities. The use of amiodarone is associated with ultrastructural changes affecting body tissues, but its effect on the ultrastructure of the heart has not yet been fully elucidated.

Methods: The aim of this study is to test the adverse effects of amiodarone administration on cardiomyocytes and to study the possible protective role of vitamin E co-administration. A total of 18 adult male albino rats were used in this study. The rats were divided randomly into three groups of 6 rats each as follows: group I was considered the control group and was given vegetable oil; group II received 54 mg/kg of oral amiodarone; and group III received a single dose of combined vitamin E (50 mg/kg) and amiodarone (54 mg/kg). After 2 weeks, the rats were sacrificed, and the atrial tissues were harvested and processed for electron microscopic study.

Results: ADMINISTRATION OF AMIODARONE ALONE MODIFIED THE ATRIAL ARCHITECTURE, WHICH WAS DEMONSTRATED BY THE FOLLOWING: mitochondrial enlargement and cristae lysis; marked heterogeneity of myofibril patterns with partial necrosis and disintegration of myofilaments; and irregularities of the sarcomere and less concentration of atrionatriuretic factor (ANF) granules, which localised in closed proximity to the nucleus with disrupted chromatin contents. Concomitant administration of vitamin E with amiodarone showed a considerable preservation of the atrial architecture.

Conclusions: Oral administration of amiodarone in rats resulted in ultrastructural changes in atria, which can be attenuated by vitamin E co-administration.

No MeSH data available.


Related in: MedlinePlus