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Enhanced decolourization of the azo dye Sirius rose BB by laccase – HBT system

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ABSTRACT

Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.

No MeSH data available.


3D surface plot for the removal of Sirius rose (SR) by laccase as a function of 1 hydroxybenzotriazole (HBT) and dye concentrations, at a fixed value of 0.5 U/mL laccase
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Fig3: 3D surface plot for the removal of Sirius rose (SR) by laccase as a function of 1 hydroxybenzotriazole (HBT) and dye concentrations, at a fixed value of 0.5 U/mL laccase

Mentions: Following validation of the model, the response surface and the isoresponse curves were prepared by plotting the response variation against two of variable factors, whilst a third factor was held constant at its mean level (0.5 U/mL of laccase, 0.5 mM HBT and 60 mg/L dye, pH 4.5 and 45 °C). The effects of these variables on decolouration of Sirius rose are shown in Figs. 2, 3, 4, 56.Fig. 2


Enhanced decolourization of the azo dye Sirius rose BB by laccase – HBT system
3D surface plot for the removal of Sirius rose (SR) by laccase as a function of 1 hydroxybenzotriazole (HBT) and dye concentrations, at a fixed value of 0.5 U/mL laccase
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376867&req=5

Fig3: 3D surface plot for the removal of Sirius rose (SR) by laccase as a function of 1 hydroxybenzotriazole (HBT) and dye concentrations, at a fixed value of 0.5 U/mL laccase
Mentions: Following validation of the model, the response surface and the isoresponse curves were prepared by plotting the response variation against two of variable factors, whilst a third factor was held constant at its mean level (0.5 U/mL of laccase, 0.5 mM HBT and 60 mg/L dye, pH 4.5 and 45 °C). The effects of these variables on decolouration of Sirius rose are shown in Figs. 2, 3, 4, 56.Fig. 2

View Article: PubMed Central

ABSTRACT

Response surface methodology (RSM) was applied to optimize the decolourization of the diazo dye Sirius rose BB (SR) by crude laccase from the white-rot fungus Trametes sp. strain CLBE55. A Box–Behnken design using RSM with six variables, namely pH, incubation temperature, enzyme (laccase) concentration, 1-hydroxybenzotriazol (HBT) concentration, dye concentration and incubation time was used in this study to determine significant correlations between the effects of these variables on the decolourization of Sirius rose. The optimum concentrations of HBT, dye and laccase were 0.5 mM, 60 mg/L and 0.1 U/mL, respectively, to obtain maximum decolourization of Sirius rose (approx. 99.5% in 150 min at 45 °C, pH 3). A quadratic model was obtained for dye decolourization using this design. Experimental values were in good agreement with values predicted by the model, giving highly significant correlations.

No MeSH data available.