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Impact of syringaldehyde on the growth of Clostridium beijerinckii NCIMB 8052 and butanol production

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ABSTRACT

While lignocellulosic biomass excels as a cheap, renewable resource for biofuel production, it does present some challenges such as generation of microbial inhibitory compounds. The mode of selective inhibition of acetone–butanol–ethanol (ABE) production (as opposed to cell growth) by syringaldehyde on Clostridium beijerinckii NCIMB 8052 was examined. C. beijerinckii 8052 grown in syringaldehyde-supplemented P2 medium had a comparable growth rate (μ = 0.34) at acidogenic growth phase to that of C. beijerinckii 8052 grown in control P2 medium (μ = 0.30). The addition of syringaldehyde into P2 medium inhibited solvent production by C. beijerinckii 8052 and increased butyric and acetic acid accumulation in the fermentation broth. Analysis of coenzyme A transferase (CoAT) using cell-free extracts of C. beijerinckii 8052 showed decreased expression and activity in the presence of syringaldehyde. These results indicate that C. beijerinckii 8052 CoAT is negatively affected by syringaldehyde and thus, hampers the ability of the microorganism to metabolize butyric and acetic acid for ABE production as evidenced by the accumulation of butyric and acetic acid in the fermentation broth.

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Effect of syringaldehyde on the activity of C. beijerinckii NCIMB 8052 CoAT. Cell-free extracts of C. beijerinckii were harvested following 12, 24, and 36 h of fermentation in control P2 medium. To obtain CTL_CoAT column, CoAT activity was assayed in the absence of syringaldehyde (control). For SA_CoAT column, syringaldehyde (1.0 g/L) was mixed CoAT prior to activity reaction assay
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Fig6: Effect of syringaldehyde on the activity of C. beijerinckii NCIMB 8052 CoAT. Cell-free extracts of C. beijerinckii were harvested following 12, 24, and 36 h of fermentation in control P2 medium. To obtain CTL_CoAT column, CoAT activity was assayed in the absence of syringaldehyde (control). For SA_CoAT column, syringaldehyde (1.0 g/L) was mixed CoAT prior to activity reaction assay

Mentions: Furthermore, cell-free extracts of C. beijerinckii grown in control P2 medium harvested following fermentation times of 12, 24 and 36 h were assayed for CoAT activity in the presence of 1.0 g/L syringaldehyde. This assay was performed to determine in vitro if expressed CoAT experienced inhibition by syringaldehyde. In the presence of syringaldehyde, cell-free extracts of C. beijerinckii grown in control P2 medium and harvested at 12, 24 and 36 h fermentation had CoAT activities of 0.8, 0.9 and 0.8 units/min/mg protein, respectively (Fig. 6). This represents a 66–68% decrease in the activity when compared to the activity of the enzyme in the absence of syringaldehyde (Fig. 6), indicating that the activity of the expressed CoAT was negatively affected by syringaldehyde (Fig. 6). This suggests that syringaldehyde can negatively affect CoAT during ABE fermentation by C. beijerinckii in two ways, first by decreasing the expression during the growth (Table 3) and second by inhibiting the activity of CoAT following expression.Fig. 6


Impact of syringaldehyde on the growth of Clostridium beijerinckii NCIMB 8052 and butanol production
Effect of syringaldehyde on the activity of C. beijerinckii NCIMB 8052 CoAT. Cell-free extracts of C. beijerinckii were harvested following 12, 24, and 36 h of fermentation in control P2 medium. To obtain CTL_CoAT column, CoAT activity was assayed in the absence of syringaldehyde (control). For SA_CoAT column, syringaldehyde (1.0 g/L) was mixed CoAT prior to activity reaction assay
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Related In: Results  -  Collection

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Fig6: Effect of syringaldehyde on the activity of C. beijerinckii NCIMB 8052 CoAT. Cell-free extracts of C. beijerinckii were harvested following 12, 24, and 36 h of fermentation in control P2 medium. To obtain CTL_CoAT column, CoAT activity was assayed in the absence of syringaldehyde (control). For SA_CoAT column, syringaldehyde (1.0 g/L) was mixed CoAT prior to activity reaction assay
Mentions: Furthermore, cell-free extracts of C. beijerinckii grown in control P2 medium harvested following fermentation times of 12, 24 and 36 h were assayed for CoAT activity in the presence of 1.0 g/L syringaldehyde. This assay was performed to determine in vitro if expressed CoAT experienced inhibition by syringaldehyde. In the presence of syringaldehyde, cell-free extracts of C. beijerinckii grown in control P2 medium and harvested at 12, 24 and 36 h fermentation had CoAT activities of 0.8, 0.9 and 0.8 units/min/mg protein, respectively (Fig. 6). This represents a 66–68% decrease in the activity when compared to the activity of the enzyme in the absence of syringaldehyde (Fig. 6), indicating that the activity of the expressed CoAT was negatively affected by syringaldehyde (Fig. 6). This suggests that syringaldehyde can negatively affect CoAT during ABE fermentation by C. beijerinckii in two ways, first by decreasing the expression during the growth (Table 3) and second by inhibiting the activity of CoAT following expression.Fig. 6

View Article: PubMed Central

ABSTRACT

While lignocellulosic biomass excels as a cheap, renewable resource for biofuel production, it does present some challenges such as generation of microbial inhibitory compounds. The mode of selective inhibition of acetone–butanol–ethanol (ABE) production (as opposed to cell growth) by syringaldehyde on Clostridium beijerinckii NCIMB 8052 was examined. C. beijerinckii 8052 grown in syringaldehyde-supplemented P2 medium had a comparable growth rate (μ = 0.34) at acidogenic growth phase to that of C. beijerinckii 8052 grown in control P2 medium (μ = 0.30). The addition of syringaldehyde into P2 medium inhibited solvent production by C. beijerinckii 8052 and increased butyric and acetic acid accumulation in the fermentation broth. Analysis of coenzyme A transferase (CoAT) using cell-free extracts of C. beijerinckii 8052 showed decreased expression and activity in the presence of syringaldehyde. These results indicate that C. beijerinckii 8052 CoAT is negatively affected by syringaldehyde and thus, hampers the ability of the microorganism to metabolize butyric and acetic acid for ABE production as evidenced by the accumulation of butyric and acetic acid in the fermentation broth.

No MeSH data available.


Related in: MedlinePlus