Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Bottom Line: In patient iPSC-derived cardiomyocytes, catecholaminergic stress led to elevated diastolic Ca(2+) concentrations, a reduced SR Ca(2+) content and an increased susceptibility to DADs and arrhythmia as compared to control myocytes.Dantrolene, a drug effective on malignant hyperthermia, restored normal Ca(2+) spark properties and rescued the arrhythmogenic phenotype.Our work provides a new in vitro model to study the pathogenesis of human cardiac arrhythmias and develop novel therapies for CPVT.
Affiliation: Klinikum rechts der Isar, Technische Universität München, I. Medizinische Klinik, Kardiologie, München, Germany.Show MeSH
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Mentions: Dermal fibroblasts were obtained from a 24-year-old woman with a diagnosis of familial CPVT, who underwent cardiac arrest at the age of 23 years and received an ICD after cardiac resuscitation. Genetic screening showed that she carried a novel autosomal dominant S406L missense mutation in the RYR2 gene, caused by a C → T nucleotide substitution in exon 14 at position 1217 of the coding region (Fig 1A and B). The mutation is located in the N-terminal domain (amino acids 1–600) of the RYR2 Ca2+ release channel, which represents, together with the central domain (amino acids 2000–2500) and the carboxy-terminal transmembrane domain, one of the three hotspots for CPVT-associated RYR2 mutations (George et al, 2007; Thomas et al, 2010; Fig 1C). Fibroblast transduction with retroviral vectors encoding for SOX2, OCT4, KLF4 and c-MYC generated several CPVT patient-specific iPSC clones, three of which were further characterized and used for cardiomyocyte differentiation. Similarly, control iPSCs were created using fibroblasts from a 32-year-old healthy female (Moretti et al, 2010b). The S406L heterozygous mutation was identified exclusively in CPVT-iPSCs. All iPSC lines showed human embryonic stem cell morphology, expression of the pluripotency markers NANOG and TRA1-81, alkaline phosphatase activity, reactivation of endogenous pluripotency genes (OCT4, SOX2, NANOG, REX1 and TDGF1), silencing of the four retroviral transgenes and normal karyotype (Fig 1D–F; Fig S1A and B of Supporting information). Pluripotency of each iPSC line was assessed by upregulation of genes specific of all three germ layers in in vitro-differentiating embryoid bodies (EBs) (Fig S1C of Supporting information).
Affiliation: Klinikum rechts der Isar, Technische Universität München, I. Medizinische Klinik, Kardiologie, München, Germany.