Limits...
Calicivirus from novel Recovirus genogroup in human diarrhea, Bangladesh.

Smits SL, Rahman M, Schapendonk CM, van Leeuwen M, Faruque AS, Haagmans BL, Endtz HP, Osterhaus AD - Emerging Infect. Dis. (2012)

Bottom Line: To identify unknown human viruses in the enteric tract, we examined 105 stool specimens from patients with diarrhea in Bangladesh.A novel calicivirus was identified in a sample from 1 patient and subsequently found in samples from 5 other patients.Phylogenetic analyses classified this virus within the proposed genus Recovirus.

View Article: PubMed Central - PubMed

Affiliation: Erasmus Medical Center, Rotterdam, the Netherlands. smits@viroclinics.com

ABSTRACT
To identify unknown human viruses in the enteric tract, we examined 105 stool specimens from patients with diarrhea in Bangladesh. A novel calicivirus was identified in a sample from 1 patient and subsequently found in samples from 5 other patients. Phylogenetic analyses classified this virus within the proposed genus Recovirus.

Show MeSH

Related in: MedlinePlus

Neighbor-joining phylogenetic trees of the amino acid sequences of the partial polyprotein sequence (A), viral protein (VP) 1 (B), and VP2 (C) capsid proteins of selected representative caliciviruses and the newly identified Recovirus Bangladesh/289/2007 (indicated in boldface). Phylograms were generated by using MEGA5 (www.megasoftware.net) with p-distance and 1,000 bootstrap replicates. Significant bootstrap values and GenBank accession numbers are shown. Scale bars indicate amino acid substitutions per site.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3376821&req=5

Figure 2: Neighbor-joining phylogenetic trees of the amino acid sequences of the partial polyprotein sequence (A), viral protein (VP) 1 (B), and VP2 (C) capsid proteins of selected representative caliciviruses and the newly identified Recovirus Bangladesh/289/2007 (indicated in boldface). Phylograms were generated by using MEGA5 (www.megasoftware.net) with p-distance and 1,000 bootstrap replicates. Significant bootstrap values and GenBank accession numbers are shown. Scale bars indicate amino acid substitutions per site.

Mentions: The Caliciviridae genome encodes a polyprotein precursor for nonstructural proteins, and 2 structural capsid proteins, viral protein (VP) 1 and VP2, from 2 or 3 open reading frames (ORFs) (Figure 1, panel B) (8,9). The genome organization of Bangladesh/289/2007 is most closely related to that of viruses in the genus Norovirus, with ORF2 encoding VP1 overlapping with ORF1 (Figure 1). The partial polyprotein precursor and complete VP1 and VP2 proteins were aligned with corresponding sequences of representative caliciviruses. Divergence analysis demonstrated that the calicivirus Bangladesh/289/2007 showed 57.7%, 49.5%, and 53.1% amino acid identity in the polyprotein, VP1, and VP2, respectively, to its closest relative, Recovirus Tulane (GenBank accession no. EU391643), which was identified from rhesus macaques (Table 1; Figure 2).


Calicivirus from novel Recovirus genogroup in human diarrhea, Bangladesh.

Smits SL, Rahman M, Schapendonk CM, van Leeuwen M, Faruque AS, Haagmans BL, Endtz HP, Osterhaus AD - Emerging Infect. Dis. (2012)

Neighbor-joining phylogenetic trees of the amino acid sequences of the partial polyprotein sequence (A), viral protein (VP) 1 (B), and VP2 (C) capsid proteins of selected representative caliciviruses and the newly identified Recovirus Bangladesh/289/2007 (indicated in boldface). Phylograms were generated by using MEGA5 (www.megasoftware.net) with p-distance and 1,000 bootstrap replicates. Significant bootstrap values and GenBank accession numbers are shown. Scale bars indicate amino acid substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376821&req=5

Figure 2: Neighbor-joining phylogenetic trees of the amino acid sequences of the partial polyprotein sequence (A), viral protein (VP) 1 (B), and VP2 (C) capsid proteins of selected representative caliciviruses and the newly identified Recovirus Bangladesh/289/2007 (indicated in boldface). Phylograms were generated by using MEGA5 (www.megasoftware.net) with p-distance and 1,000 bootstrap replicates. Significant bootstrap values and GenBank accession numbers are shown. Scale bars indicate amino acid substitutions per site.
Mentions: The Caliciviridae genome encodes a polyprotein precursor for nonstructural proteins, and 2 structural capsid proteins, viral protein (VP) 1 and VP2, from 2 or 3 open reading frames (ORFs) (Figure 1, panel B) (8,9). The genome organization of Bangladesh/289/2007 is most closely related to that of viruses in the genus Norovirus, with ORF2 encoding VP1 overlapping with ORF1 (Figure 1). The partial polyprotein precursor and complete VP1 and VP2 proteins were aligned with corresponding sequences of representative caliciviruses. Divergence analysis demonstrated that the calicivirus Bangladesh/289/2007 showed 57.7%, 49.5%, and 53.1% amino acid identity in the polyprotein, VP1, and VP2, respectively, to its closest relative, Recovirus Tulane (GenBank accession no. EU391643), which was identified from rhesus macaques (Table 1; Figure 2).

Bottom Line: To identify unknown human viruses in the enteric tract, we examined 105 stool specimens from patients with diarrhea in Bangladesh.A novel calicivirus was identified in a sample from 1 patient and subsequently found in samples from 5 other patients.Phylogenetic analyses classified this virus within the proposed genus Recovirus.

View Article: PubMed Central - PubMed

Affiliation: Erasmus Medical Center, Rotterdam, the Netherlands. smits@viroclinics.com

ABSTRACT
To identify unknown human viruses in the enteric tract, we examined 105 stool specimens from patients with diarrhea in Bangladesh. A novel calicivirus was identified in a sample from 1 patient and subsequently found in samples from 5 other patients. Phylogenetic analyses classified this virus within the proposed genus Recovirus.

Show MeSH
Related in: MedlinePlus