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Outbreak-associated novel duck Reovirus, China, 2011.

Chen Z, Zhu Y, Li C, Liu G - Emerging Infect. Dis. (2012)

View Article: PubMed Central - PubMed

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In 2011, an unidentified disease in Pekin ducks (Anas platyrhynchos) was reported in People’s Republic of China... The virions are nonenveloped, with icosahedral symmetry and a double capsid containing 10 double-stranded RNA segments that can be separated by polyacrylamide gel electrophoresis into 3 size classes: large (L1–L3), medium (M1–M3), and small (S1–S4)... ARVs cause a range of diseases in chicken, including viral arthritis/tenosynovitis, and are associated with respiratory disease, enteric disease, inclusion body hepatitis, hydropericardium, runting stunting syndrome, malabsorption syndrome, and sudden death... Muscovy duck reovirus infection caused illness in 30% and death in 20% of ducks on poultry farms in Israel... In China, reovirus infection has been reported in Muscovy ducklings, with a resulting death rate of 10%–30% since 1997... Since 2007, three isolates of orthoreovirus were confirmed in Pekin ducks from several duck farms in China... However, experiment infection with the isolates did not cause death... In 2011, farmers and veterinarians in China reported to the Animal Health Services and National Research Institutes an unidentified disease in ducks that spread rapidly around the county... Virus isolation from liver homogenate was conducted in duck embryo fibroblast cultures... At 48 hours after infection, a strong cytopathic effect was observed, including syncytium formation... JQ664689) was aligned with 30 published orthoreovirus sequences, including data on all 3 newly obtained sequences from Pekin duck reovirus in China in 2008 and 2011... In summary, we isolated a novel duck-pathogenic orthoreovirus from the liver of affected Pekin ducks... This finding highlights the need to prevent and control this highly transmissible infectious agent... Further study is needed to determine what role the newly isolated DRV played in the 2011 outbreaks on many of the duck farms in China.

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Electron micrographs of purified DRV-TH11 isolate particles showing the features of orthoreovirus. Original magnification ×100,000. Scale bar = 100 nm.
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FA.1: Electron micrographs of purified DRV-TH11 isolate particles showing the features of orthoreovirus. Original magnification ×100,000. Scale bar = 100 nm.

Mentions: Since 2007, three isolates of orthoreovirus were confirmed in Pekin ducks from several duck farms in China. However, experiment infection with the isolates did not cause death (6). In 2011, farmers and veterinarians in China reported to the Animal Health Services and National Research Institutes an unidentified disease in ducks that spread rapidly around the county. We conducted further investigation to identify the causative agent of this disease. The diseased ducks showed depression and leg weakness. Large necrotic foci were observed in the spleens of the dead ducks. Histopathologic examination showed necrotic foci and granulomas in the spleen. Focal hepatic necrosis and proliferation of bile ducts were seen in the liver. Virus isolation from liver homogenate was conducted in duck embryo fibroblast cultures. At 48 hours after infection, a strong cytopathic effect was observed, including syncytium formation. All duck embryos experimentally infected with the isolate died within 48–72 hours after infection. The dead embryos showed swollen livers with petechial hemorrhages. Spherical, spiked virus particles, consistent with those of members of the family Reoviridae, were observed by electron microscopy. As reported (7), the diameter of the particles was ≈85 nm (Figure A1). The RNA extracted from DRV-infected duck embryo fibroblast cultures showed 10 dsRNA segments in 3 size classes (L1–3, M1–3, and S1–4) on polyacrylamide gel electrophoresis. The isolate was designated as novel duck reovirus, DRV-TH11. The pathogenicity of DRV-TH11 was tested by infecting 10-day-old Pekin ducks subcutaneously at a dose of 4 × 104.5 50% tissue culture infective dose. Experimental infection caused death on day 3 after infection. The clinical signs and histopathologic examination show the same features as the naturally infected ducks.


Outbreak-associated novel duck Reovirus, China, 2011.

Chen Z, Zhu Y, Li C, Liu G - Emerging Infect. Dis. (2012)

Electron micrographs of purified DRV-TH11 isolate particles showing the features of orthoreovirus. Original magnification ×100,000. Scale bar = 100 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376814&req=5

FA.1: Electron micrographs of purified DRV-TH11 isolate particles showing the features of orthoreovirus. Original magnification ×100,000. Scale bar = 100 nm.
Mentions: Since 2007, three isolates of orthoreovirus were confirmed in Pekin ducks from several duck farms in China. However, experiment infection with the isolates did not cause death (6). In 2011, farmers and veterinarians in China reported to the Animal Health Services and National Research Institutes an unidentified disease in ducks that spread rapidly around the county. We conducted further investigation to identify the causative agent of this disease. The diseased ducks showed depression and leg weakness. Large necrotic foci were observed in the spleens of the dead ducks. Histopathologic examination showed necrotic foci and granulomas in the spleen. Focal hepatic necrosis and proliferation of bile ducts were seen in the liver. Virus isolation from liver homogenate was conducted in duck embryo fibroblast cultures. At 48 hours after infection, a strong cytopathic effect was observed, including syncytium formation. All duck embryos experimentally infected with the isolate died within 48–72 hours after infection. The dead embryos showed swollen livers with petechial hemorrhages. Spherical, spiked virus particles, consistent with those of members of the family Reoviridae, were observed by electron microscopy. As reported (7), the diameter of the particles was ≈85 nm (Figure A1). The RNA extracted from DRV-infected duck embryo fibroblast cultures showed 10 dsRNA segments in 3 size classes (L1–3, M1–3, and S1–4) on polyacrylamide gel electrophoresis. The isolate was designated as novel duck reovirus, DRV-TH11. The pathogenicity of DRV-TH11 was tested by infecting 10-day-old Pekin ducks subcutaneously at a dose of 4 × 104.5 50% tissue culture infective dose. Experimental infection caused death on day 3 after infection. The clinical signs and histopathologic examination show the same features as the naturally infected ducks.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

In 2011, an unidentified disease in Pekin ducks (Anas platyrhynchos) was reported in People’s Republic of China... The virions are nonenveloped, with icosahedral symmetry and a double capsid containing 10 double-stranded RNA segments that can be separated by polyacrylamide gel electrophoresis into 3 size classes: large (L1–L3), medium (M1–M3), and small (S1–S4)... ARVs cause a range of diseases in chicken, including viral arthritis/tenosynovitis, and are associated with respiratory disease, enteric disease, inclusion body hepatitis, hydropericardium, runting stunting syndrome, malabsorption syndrome, and sudden death... Muscovy duck reovirus infection caused illness in 30% and death in 20% of ducks on poultry farms in Israel... In China, reovirus infection has been reported in Muscovy ducklings, with a resulting death rate of 10%–30% since 1997... Since 2007, three isolates of orthoreovirus were confirmed in Pekin ducks from several duck farms in China... However, experiment infection with the isolates did not cause death... In 2011, farmers and veterinarians in China reported to the Animal Health Services and National Research Institutes an unidentified disease in ducks that spread rapidly around the county... Virus isolation from liver homogenate was conducted in duck embryo fibroblast cultures... At 48 hours after infection, a strong cytopathic effect was observed, including syncytium formation... JQ664689) was aligned with 30 published orthoreovirus sequences, including data on all 3 newly obtained sequences from Pekin duck reovirus in China in 2008 and 2011... In summary, we isolated a novel duck-pathogenic orthoreovirus from the liver of affected Pekin ducks... This finding highlights the need to prevent and control this highly transmissible infectious agent... Further study is needed to determine what role the newly isolated DRV played in the 2011 outbreaks on many of the duck farms in China.

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