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Outbreak-associated novel duck Reovirus, China, 2011.

Chen Z, Zhu Y, Li C, Liu G - Emerging Infect. Dis. (2012)

View Article: PubMed Central - PubMed

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In 2011, an unidentified disease in Pekin ducks (Anas platyrhynchos) was reported in People’s Republic of China... The virions are nonenveloped, with icosahedral symmetry and a double capsid containing 10 double-stranded RNA segments that can be separated by polyacrylamide gel electrophoresis into 3 size classes: large (L1–L3), medium (M1–M3), and small (S1–S4)... ARVs cause a range of diseases in chicken, including viral arthritis/tenosynovitis, and are associated with respiratory disease, enteric disease, inclusion body hepatitis, hydropericardium, runting stunting syndrome, malabsorption syndrome, and sudden death... Muscovy duck reovirus infection caused illness in 30% and death in 20% of ducks on poultry farms in Israel... In China, reovirus infection has been reported in Muscovy ducklings, with a resulting death rate of 10%–30% since 1997... Since 2007, three isolates of orthoreovirus were confirmed in Pekin ducks from several duck farms in China... However, experiment infection with the isolates did not cause death... In 2011, farmers and veterinarians in China reported to the Animal Health Services and National Research Institutes an unidentified disease in ducks that spread rapidly around the county... Virus isolation from liver homogenate was conducted in duck embryo fibroblast cultures... At 48 hours after infection, a strong cytopathic effect was observed, including syncytium formation... JQ664689) was aligned with 30 published orthoreovirus sequences, including data on all 3 newly obtained sequences from Pekin duck reovirus in China in 2008 and 2011... In summary, we isolated a novel duck-pathogenic orthoreovirus from the liver of affected Pekin ducks... This finding highlights the need to prevent and control this highly transmissible infectious agent... Further study is needed to determine what role the newly isolated DRV played in the 2011 outbreaks on many of the duck farms in China.

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Phylogenetic relationship between DRV-TH11 isolate and orthoreovirus of the avian orthoreovirus (ARV) and mammalian orthoreovirus (MRV). ARV includes chicken reovirus, Muscovy duck reovirus, and Pekin duck reovirus. GenBank accession numbers of the sequences in the analysis are indicated in the tree. The neighbor-joining tree is based on the complete sequence of s2 gene (1,251 nt). Numbers at nodes represent the percentage of 1,000 bootstrap replicates (values <50 are not shown). Scale bar indicates a branch length corresponding to 100 character-state changes.
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Figure 1: Phylogenetic relationship between DRV-TH11 isolate and orthoreovirus of the avian orthoreovirus (ARV) and mammalian orthoreovirus (MRV). ARV includes chicken reovirus, Muscovy duck reovirus, and Pekin duck reovirus. GenBank accession numbers of the sequences in the analysis are indicated in the tree. The neighbor-joining tree is based on the complete sequence of s2 gene (1,251 nt). Numbers at nodes represent the percentage of 1,000 bootstrap replicates (values <50 are not shown). Scale bar indicates a branch length corresponding to 100 character-state changes.

Mentions: For phylogenetic analyses, the S2 gene was amplified by reverse transcription PCR with avian reovirus–specific primers. The complete sequence of the S2 gene (GenBank accession no. JQ664689) was aligned with 30 published orthoreovirus sequences, including data on all 3 newly obtained sequences from Pekin duck reovirus in China in 2008 and 2011. Phylogenetic relationship was assessed by using the neighbor-joining method based on a Tamura 3-parameter model and bootstrap analysis (1,000 replicates) as implemented in MEGA5 (8). The phylogenetic tree shows that the complete sequence of S2 gene is distinct but clusters closely with sequences from all 3 Pekin duck isolates within the ARVs serogroup, which suggests that the novel virus is an ARV-like virus within the genus Orthoreovirus (Figure).


Outbreak-associated novel duck Reovirus, China, 2011.

Chen Z, Zhu Y, Li C, Liu G - Emerging Infect. Dis. (2012)

Phylogenetic relationship between DRV-TH11 isolate and orthoreovirus of the avian orthoreovirus (ARV) and mammalian orthoreovirus (MRV). ARV includes chicken reovirus, Muscovy duck reovirus, and Pekin duck reovirus. GenBank accession numbers of the sequences in the analysis are indicated in the tree. The neighbor-joining tree is based on the complete sequence of s2 gene (1,251 nt). Numbers at nodes represent the percentage of 1,000 bootstrap replicates (values <50 are not shown). Scale bar indicates a branch length corresponding to 100 character-state changes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376814&req=5

Figure 1: Phylogenetic relationship between DRV-TH11 isolate and orthoreovirus of the avian orthoreovirus (ARV) and mammalian orthoreovirus (MRV). ARV includes chicken reovirus, Muscovy duck reovirus, and Pekin duck reovirus. GenBank accession numbers of the sequences in the analysis are indicated in the tree. The neighbor-joining tree is based on the complete sequence of s2 gene (1,251 nt). Numbers at nodes represent the percentage of 1,000 bootstrap replicates (values <50 are not shown). Scale bar indicates a branch length corresponding to 100 character-state changes.
Mentions: For phylogenetic analyses, the S2 gene was amplified by reverse transcription PCR with avian reovirus–specific primers. The complete sequence of the S2 gene (GenBank accession no. JQ664689) was aligned with 30 published orthoreovirus sequences, including data on all 3 newly obtained sequences from Pekin duck reovirus in China in 2008 and 2011. Phylogenetic relationship was assessed by using the neighbor-joining method based on a Tamura 3-parameter model and bootstrap analysis (1,000 replicates) as implemented in MEGA5 (8). The phylogenetic tree shows that the complete sequence of S2 gene is distinct but clusters closely with sequences from all 3 Pekin duck isolates within the ARVs serogroup, which suggests that the novel virus is an ARV-like virus within the genus Orthoreovirus (Figure).

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

In 2011, an unidentified disease in Pekin ducks (Anas platyrhynchos) was reported in People’s Republic of China... The virions are nonenveloped, with icosahedral symmetry and a double capsid containing 10 double-stranded RNA segments that can be separated by polyacrylamide gel electrophoresis into 3 size classes: large (L1–L3), medium (M1–M3), and small (S1–S4)... ARVs cause a range of diseases in chicken, including viral arthritis/tenosynovitis, and are associated with respiratory disease, enteric disease, inclusion body hepatitis, hydropericardium, runting stunting syndrome, malabsorption syndrome, and sudden death... Muscovy duck reovirus infection caused illness in 30% and death in 20% of ducks on poultry farms in Israel... In China, reovirus infection has been reported in Muscovy ducklings, with a resulting death rate of 10%–30% since 1997... Since 2007, three isolates of orthoreovirus were confirmed in Pekin ducks from several duck farms in China... However, experiment infection with the isolates did not cause death... In 2011, farmers and veterinarians in China reported to the Animal Health Services and National Research Institutes an unidentified disease in ducks that spread rapidly around the county... Virus isolation from liver homogenate was conducted in duck embryo fibroblast cultures... At 48 hours after infection, a strong cytopathic effect was observed, including syncytium formation... JQ664689) was aligned with 30 published orthoreovirus sequences, including data on all 3 newly obtained sequences from Pekin duck reovirus in China in 2008 and 2011... In summary, we isolated a novel duck-pathogenic orthoreovirus from the liver of affected Pekin ducks... This finding highlights the need to prevent and control this highly transmissible infectious agent... Further study is needed to determine what role the newly isolated DRV played in the 2011 outbreaks on many of the duck farms in China.

Show MeSH