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Potential international spread of multidrug-resistant invasive Salmonella enterica serovar enteritidis.

Rodríguez I, Rodicio MR, Guerra B, Hopkins KL - Emerging Infect. Dis. (2012)

Bottom Line: In developing countries, Salmonella enterica serovar Enteritidis causes substantial illness and death, and drug resistance is increasing.Isolates from the United Kingdom containing virulence-resistance plasmids were characterized.The common features in isolates from these continents indicate the role of human travel in their spread.

View Article: PubMed Central - PubMed

Affiliation: Federal Institute for Risk Assessment, Berlin, Germany. rodriguezfi rene@uniovi.es

ABSTRACT
In developing countries, Salmonella enterica serovar Enteritidis causes substantial illness and death, and drug resistance is increasing. Isolates from the United Kingdom containing virulence-resistance plasmids were characterized. They mainly caused invasive infections in adults linked to Africa. The common features in isolates from these continents indicate the role of human travel in their spread.

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Related in: MedlinePlus

Genomic macrorestriction of Salmonellaenterica serovar Enteritidis isolates: pulsed-field gel electrophoresis profiles for XbaI (left panel) and S1 (right panel). Lane M, XbaI-digested DNA of S. enterica serovar Braenderup H9812, used as size standard; lane 1, NRL-Salm-PT4; lane 2, CNM4839/03; lane 3, H051860415; lane 4, H070360201; lane 5, H070420137; lane 6, H073180204; lane 7, H091340084; lane 8, H091800482; lane 9, H095100307; lane 10, H100240198; lane 11, H101700366. The strain NRL-Salm-PT4 was used as control for the most commonly found XbaI-profile in S. enterica serovar Enteritidis.
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Figure 1: Genomic macrorestriction of Salmonellaenterica serovar Enteritidis isolates: pulsed-field gel electrophoresis profiles for XbaI (left panel) and S1 (right panel). Lane M, XbaI-digested DNA of S. enterica serovar Braenderup H9812, used as size standard; lane 1, NRL-Salm-PT4; lane 2, CNM4839/03; lane 3, H051860415; lane 4, H070360201; lane 5, H070420137; lane 6, H073180204; lane 7, H091340084; lane 8, H091800482; lane 9, H095100307; lane 10, H100240198; lane 11, H101700366. The strain NRL-Salm-PT4 was used as control for the most commonly found XbaI-profile in S. enterica serovar Enteritidis.

Mentions: We screened 31,615 S. enterica serovar Enteritidis isolates that had been collected from clinical specimens during 2005–2010 and deposited in the culture collection of the Health Protection Agency Salmonella Reference Unit. We screened the isolates for R-type ACSSuTTm. A total of 14 serovar Enteritidis isolates showing this resistance phenotype were detected and subsequently examined for the presence of integron-located dfrA7. Of the 14 isolates, 11 were positive and their plasmid content was analyzed by S1 pulsed-field gel electrophoresis (PFGE) (2) and by the Kado and Liu methods (7); we used serovar Enteritidis strains NRL-Salm-PT4 and CNM4839/03 as controls for pSEV– and pUO-SeVR1–carrying isolates, respectively. The 11 isolates harbored 1 plasmid of variable size (60–95 kb); among these, 9 isolates hybridized with dfrA7-specific and spvC-specific probes (with plasmids of 85–95 kb) (Figure). These 9 isolates contained a VR-hybrid plasmid similar to pUO-SeVR1 and were selected for further analyses (Table 1, Table 2). The remaining 2 isolates carried the normal pSEV plasmid (60 kb), in which spvC hybridized; dfrA7 was chromosomally located.


Potential international spread of multidrug-resistant invasive Salmonella enterica serovar enteritidis.

Rodríguez I, Rodicio MR, Guerra B, Hopkins KL - Emerging Infect. Dis. (2012)

Genomic macrorestriction of Salmonellaenterica serovar Enteritidis isolates: pulsed-field gel electrophoresis profiles for XbaI (left panel) and S1 (right panel). Lane M, XbaI-digested DNA of S. enterica serovar Braenderup H9812, used as size standard; lane 1, NRL-Salm-PT4; lane 2, CNM4839/03; lane 3, H051860415; lane 4, H070360201; lane 5, H070420137; lane 6, H073180204; lane 7, H091340084; lane 8, H091800482; lane 9, H095100307; lane 10, H100240198; lane 11, H101700366. The strain NRL-Salm-PT4 was used as control for the most commonly found XbaI-profile in S. enterica serovar Enteritidis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376808&req=5

Figure 1: Genomic macrorestriction of Salmonellaenterica serovar Enteritidis isolates: pulsed-field gel electrophoresis profiles for XbaI (left panel) and S1 (right panel). Lane M, XbaI-digested DNA of S. enterica serovar Braenderup H9812, used as size standard; lane 1, NRL-Salm-PT4; lane 2, CNM4839/03; lane 3, H051860415; lane 4, H070360201; lane 5, H070420137; lane 6, H073180204; lane 7, H091340084; lane 8, H091800482; lane 9, H095100307; lane 10, H100240198; lane 11, H101700366. The strain NRL-Salm-PT4 was used as control for the most commonly found XbaI-profile in S. enterica serovar Enteritidis.
Mentions: We screened 31,615 S. enterica serovar Enteritidis isolates that had been collected from clinical specimens during 2005–2010 and deposited in the culture collection of the Health Protection Agency Salmonella Reference Unit. We screened the isolates for R-type ACSSuTTm. A total of 14 serovar Enteritidis isolates showing this resistance phenotype were detected and subsequently examined for the presence of integron-located dfrA7. Of the 14 isolates, 11 were positive and their plasmid content was analyzed by S1 pulsed-field gel electrophoresis (PFGE) (2) and by the Kado and Liu methods (7); we used serovar Enteritidis strains NRL-Salm-PT4 and CNM4839/03 as controls for pSEV– and pUO-SeVR1–carrying isolates, respectively. The 11 isolates harbored 1 plasmid of variable size (60–95 kb); among these, 9 isolates hybridized with dfrA7-specific and spvC-specific probes (with plasmids of 85–95 kb) (Figure). These 9 isolates contained a VR-hybrid plasmid similar to pUO-SeVR1 and were selected for further analyses (Table 1, Table 2). The remaining 2 isolates carried the normal pSEV plasmid (60 kb), in which spvC hybridized; dfrA7 was chromosomally located.

Bottom Line: In developing countries, Salmonella enterica serovar Enteritidis causes substantial illness and death, and drug resistance is increasing.Isolates from the United Kingdom containing virulence-resistance plasmids were characterized.The common features in isolates from these continents indicate the role of human travel in their spread.

View Article: PubMed Central - PubMed

Affiliation: Federal Institute for Risk Assessment, Berlin, Germany. rodriguezfi rene@uniovi.es

ABSTRACT
In developing countries, Salmonella enterica serovar Enteritidis causes substantial illness and death, and drug resistance is increasing. Isolates from the United Kingdom containing virulence-resistance plasmids were characterized. They mainly caused invasive infections in adults linked to Africa. The common features in isolates from these continents indicate the role of human travel in their spread.

Show MeSH
Related in: MedlinePlus