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Disseminated microsporidiosis in an immunosuppressed patient.

Meissner EG, Bennett JE, Qvarnstrom Y, da Silva A, Chu EY, Tsokos M, Gea-Banacloche J - Emerging Infect. Dis. (2012)

Bottom Line: We report a case of disseminated microsporidiosis in a patient with multiple myeloma who had received an allogeneic stem cell transplant requiring substantial immunosuppression.The causative organism was identified as Tubulinosema acridophagus, confirming this genus of microsporidia as a novel human pathogen.

View Article: PubMed Central - PubMed

Affiliation: National Institutes of Health, Bethesda, Maryland 20892, USA. eric.meissner@nih.gov

ABSTRACT
We report a case of disseminated microsporidiosis in a patient with multiple myeloma who had received an allogeneic stem cell transplant requiring substantial immunosuppression. The causative organism was identified as Tubulinosema acridophagus, confirming this genus of microsporidia as a novel human pathogen.

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Related in: MedlinePlus

Cladogram of Tubulinosematidae spp. based on small subunit ribosomal RNA gene sequences. Nosema apis was added as an outgroup. The phylogenetic tree was created by using the quartet puzzling maximum likelihood program TREE-PUZZLE (www.tree-puzzle.de). The numbers at the nodes are quartet puzzling estimations of support to each internal branch.
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Figure 2: Cladogram of Tubulinosematidae spp. based on small subunit ribosomal RNA gene sequences. Nosema apis was added as an outgroup. The phylogenetic tree was created by using the quartet puzzling maximum likelihood program TREE-PUZZLE (www.tree-puzzle.de). The numbers at the nodes are quartet puzzling estimations of support to each internal branch.

Mentions: BAL samples were sent to the reference diagnostic laboratory for parasitology at the Centers for Disease Control and Prevention for molecular analysis. A nested PCR approach was applied, using the outer primers microFN18 (5′-CACCAGGTTGATTCTGCC-3′) and microR9 (5′-GTATGATCCTGCCACAGATTCTTCTAT-3′) and the inner primers Ta50f (5′-GGTTGATTCTGCCTGTTATATGT-3′) and Ta1300r (5′-GGACACATTCATCGTAACTTAGT-3′). Phylogenetic analysis of the resulting 1,246-bp sequence (GenBank accession no. JQ247017) revealed a high similarity to the small subunit ribosomal RNA gene from T. acridophagus (Figure 2), with only 1 nucleotide difference between the 2 sequences. Our final clinical diagnosis was disseminated T. acridophagus microsporidiosis with skin, liver, peritoneal, lung, and possibly chorioretinal involvement. Histopathology was notable for a paucity of inflammation around the organisms.


Disseminated microsporidiosis in an immunosuppressed patient.

Meissner EG, Bennett JE, Qvarnstrom Y, da Silva A, Chu EY, Tsokos M, Gea-Banacloche J - Emerging Infect. Dis. (2012)

Cladogram of Tubulinosematidae spp. based on small subunit ribosomal RNA gene sequences. Nosema apis was added as an outgroup. The phylogenetic tree was created by using the quartet puzzling maximum likelihood program TREE-PUZZLE (www.tree-puzzle.de). The numbers at the nodes are quartet puzzling estimations of support to each internal branch.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376806&req=5

Figure 2: Cladogram of Tubulinosematidae spp. based on small subunit ribosomal RNA gene sequences. Nosema apis was added as an outgroup. The phylogenetic tree was created by using the quartet puzzling maximum likelihood program TREE-PUZZLE (www.tree-puzzle.de). The numbers at the nodes are quartet puzzling estimations of support to each internal branch.
Mentions: BAL samples were sent to the reference diagnostic laboratory for parasitology at the Centers for Disease Control and Prevention for molecular analysis. A nested PCR approach was applied, using the outer primers microFN18 (5′-CACCAGGTTGATTCTGCC-3′) and microR9 (5′-GTATGATCCTGCCACAGATTCTTCTAT-3′) and the inner primers Ta50f (5′-GGTTGATTCTGCCTGTTATATGT-3′) and Ta1300r (5′-GGACACATTCATCGTAACTTAGT-3′). Phylogenetic analysis of the resulting 1,246-bp sequence (GenBank accession no. JQ247017) revealed a high similarity to the small subunit ribosomal RNA gene from T. acridophagus (Figure 2), with only 1 nucleotide difference between the 2 sequences. Our final clinical diagnosis was disseminated T. acridophagus microsporidiosis with skin, liver, peritoneal, lung, and possibly chorioretinal involvement. Histopathology was notable for a paucity of inflammation around the organisms.

Bottom Line: We report a case of disseminated microsporidiosis in a patient with multiple myeloma who had received an allogeneic stem cell transplant requiring substantial immunosuppression.The causative organism was identified as Tubulinosema acridophagus, confirming this genus of microsporidia as a novel human pathogen.

View Article: PubMed Central - PubMed

Affiliation: National Institutes of Health, Bethesda, Maryland 20892, USA. eric.meissner@nih.gov

ABSTRACT
We report a case of disseminated microsporidiosis in a patient with multiple myeloma who had received an allogeneic stem cell transplant requiring substantial immunosuppression. The causative organism was identified as Tubulinosema acridophagus, confirming this genus of microsporidia as a novel human pathogen.

Show MeSH
Related in: MedlinePlus