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Influenza virus infection in guinea pigs raised as livestock, Ecuador.

Leyva-Grado VH, Mubareka S, Krammer F, Cárdenas WB, Palese P - Emerging Infect. Dis. (2012)

Bottom Line: To determine whether guinea pigs are infected with influenza virus in nature, we conducted a serologic study in domestic guinea pigs in Ecuador.Detection of antibodies against influenza A and B raises the question about the role of guinea pigs in the ecology and epidemiology of influenza virus in the region.

View Article: PubMed Central - PubMed

Affiliation: Mount Sinai School of Medicine, New York, New York 10029-6574, USA.

ABSTRACT
To determine whether guinea pigs are infected with influenza virus in nature, we conducted a serologic study in domestic guinea pigs in Ecuador. Detection of antibodies against influenza A and B raises the question about the role of guinea pigs in the ecology and epidemiology of influenza virus in the region.

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Related in: MedlinePlus

Results of Western blot analyses of pooled serum samples from adult guinea pigs in Ecuador. The guinea pigs were obtained from farms in Cuenca, where they had been raised as livestock, or from live animal markets in Guayaquil and Manabí. The results show different influenza antigens: recombinant hemagglutinin (rHA) A/New Caledonia/20/1999 (A); rHA A/Wisconsin/67/2005 (B); rHA A/Vietnam/1203/2004 (C); recombinant nucleoprotein A/Puerto Rico/08/1934 (D); and B virus (whole virus B/Yamagata/16/1988) (E). Molecular weights are shown at left. (+), serum from guinea pigs infected in the laboratory with influenza virus; (−), serum from naive guinea pig; lanes 1–4, pooled serum samples from Cuenca; lanes 5–6, pooled serum samples from Guayaquil; lanes 7–8, pooled serum samples from Manabí; S, secondary antibody only.
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Figure 2: Results of Western blot analyses of pooled serum samples from adult guinea pigs in Ecuador. The guinea pigs were obtained from farms in Cuenca, where they had been raised as livestock, or from live animal markets in Guayaquil and Manabí. The results show different influenza antigens: recombinant hemagglutinin (rHA) A/New Caledonia/20/1999 (A); rHA A/Wisconsin/67/2005 (B); rHA A/Vietnam/1203/2004 (C); recombinant nucleoprotein A/Puerto Rico/08/1934 (D); and B virus (whole virus B/Yamagata/16/1988) (E). Molecular weights are shown at left. (+), serum from guinea pigs infected in the laboratory with influenza virus; (−), serum from naive guinea pig; lanes 1–4, pooled serum samples from Cuenca; lanes 5–6, pooled serum samples from Guayaquil; lanes 7–8, pooled serum samples from Manabí; S, secondary antibody only.

Mentions: WB analysis of pooled samples from the 3 areas confirmed the ELISA results that showed antibodies against the hemagglutinin and nucleoprotein antigens; however, samples from Manabí region (7,8) showed immunoreactivity to the H3 antigen only (Figure 2). WB results for serum samples from Cuenca and Guayaquil were positive for influenza B virus, but results were not positive for samples from Manabí when whole virus was used as antigen (Figure 2). HI activity was observed in all pooled samples against all 3 viruses tested. Titers were highest in samples from Cuenca (320 for Brisbane07, 640 for Wisconsin05, and 80 for Vietnam04) and lowest for samples from Manabí (80 for Brisbane07 and 40 for Wisconsin05 and Vietnam04). Titers for samples from Guayaquil were 160 for Brisbane07 and 80 for Wisconsin05 and Vietnam04.


Influenza virus infection in guinea pigs raised as livestock, Ecuador.

Leyva-Grado VH, Mubareka S, Krammer F, Cárdenas WB, Palese P - Emerging Infect. Dis. (2012)

Results of Western blot analyses of pooled serum samples from adult guinea pigs in Ecuador. The guinea pigs were obtained from farms in Cuenca, where they had been raised as livestock, or from live animal markets in Guayaquil and Manabí. The results show different influenza antigens: recombinant hemagglutinin (rHA) A/New Caledonia/20/1999 (A); rHA A/Wisconsin/67/2005 (B); rHA A/Vietnam/1203/2004 (C); recombinant nucleoprotein A/Puerto Rico/08/1934 (D); and B virus (whole virus B/Yamagata/16/1988) (E). Molecular weights are shown at left. (+), serum from guinea pigs infected in the laboratory with influenza virus; (−), serum from naive guinea pig; lanes 1–4, pooled serum samples from Cuenca; lanes 5–6, pooled serum samples from Guayaquil; lanes 7–8, pooled serum samples from Manabí; S, secondary antibody only.
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Related In: Results  -  Collection

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Figure 2: Results of Western blot analyses of pooled serum samples from adult guinea pigs in Ecuador. The guinea pigs were obtained from farms in Cuenca, where they had been raised as livestock, or from live animal markets in Guayaquil and Manabí. The results show different influenza antigens: recombinant hemagglutinin (rHA) A/New Caledonia/20/1999 (A); rHA A/Wisconsin/67/2005 (B); rHA A/Vietnam/1203/2004 (C); recombinant nucleoprotein A/Puerto Rico/08/1934 (D); and B virus (whole virus B/Yamagata/16/1988) (E). Molecular weights are shown at left. (+), serum from guinea pigs infected in the laboratory with influenza virus; (−), serum from naive guinea pig; lanes 1–4, pooled serum samples from Cuenca; lanes 5–6, pooled serum samples from Guayaquil; lanes 7–8, pooled serum samples from Manabí; S, secondary antibody only.
Mentions: WB analysis of pooled samples from the 3 areas confirmed the ELISA results that showed antibodies against the hemagglutinin and nucleoprotein antigens; however, samples from Manabí region (7,8) showed immunoreactivity to the H3 antigen only (Figure 2). WB results for serum samples from Cuenca and Guayaquil were positive for influenza B virus, but results were not positive for samples from Manabí when whole virus was used as antigen (Figure 2). HI activity was observed in all pooled samples against all 3 viruses tested. Titers were highest in samples from Cuenca (320 for Brisbane07, 640 for Wisconsin05, and 80 for Vietnam04) and lowest for samples from Manabí (80 for Brisbane07 and 40 for Wisconsin05 and Vietnam04). Titers for samples from Guayaquil were 160 for Brisbane07 and 80 for Wisconsin05 and Vietnam04.

Bottom Line: To determine whether guinea pigs are infected with influenza virus in nature, we conducted a serologic study in domestic guinea pigs in Ecuador.Detection of antibodies against influenza A and B raises the question about the role of guinea pigs in the ecology and epidemiology of influenza virus in the region.

View Article: PubMed Central - PubMed

Affiliation: Mount Sinai School of Medicine, New York, New York 10029-6574, USA.

ABSTRACT
To determine whether guinea pigs are infected with influenza virus in nature, we conducted a serologic study in domestic guinea pigs in Ecuador. Detection of antibodies against influenza A and B raises the question about the role of guinea pigs in the ecology and epidemiology of influenza virus in the region.

Show MeSH
Related in: MedlinePlus