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Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam.

Poulsen LL, Bisgaard M, Son NT, Trung NV, An HM, Dalsgaard A - Emerging Infect. Dis. (2012)

Bottom Line: Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear.Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences.The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT
Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear. To investigate whether poultry might be a reservoir for E. faecalis-associated urinary tract infections (UTIs) in humans, we characterized E. faecalis isolates from patients in Vietnam with UTIs during January 2008-January 2010 and poultry living in close contact with them by multilocus sequence typing (MLST), pulsed-field gel electrophoresis, analysis of antimicrobial drug susceptibility patterns, and sequencing of virulence genes. In 7 (23%) of 31 UTI cases, we detected identical MLST, indistinguishable or closely related pulsed-field gel electrophoresis patterns, and similar antimicrobial drug susceptibility patterns. Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences. The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.

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Related in: MedlinePlus

Pulsed-field gel electrophoresis of SmaI-digested Enterococcus faecalis isolated from humans with urinary tract infections and from poultry in the same houselhold, Vietnam, January 2008–January 2010. Lanes A and P are molecular weight markers. Lane B, isolate 90U; lane C, isolate 90P; lane D, 122U; lane E, 122P; lane F, 186U; lane G, 186P; lane H, 191U; lane I, 191P; lane J, 204U; lane K, 204P; lane L, 217U; lane M, 217P; lane N, 221U; and lane O, 221P.
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Figure 1: Pulsed-field gel electrophoresis of SmaI-digested Enterococcus faecalis isolated from humans with urinary tract infections and from poultry in the same houselhold, Vietnam, January 2008–January 2010. Lanes A and P are molecular weight markers. Lane B, isolate 90U; lane C, isolate 90P; lane D, 122U; lane E, 122P; lane F, 186U; lane G, 186P; lane H, 191U; lane I, 191P; lane J, 204U; lane K, 204P; lane L, 217U; lane M, 217P; lane N, 221U; and lane O, 221P.

Mentions: In 7 (23%) of 31 UTI cases, E. faecalis isolated from patient urine and poultry demonstrated identical STs and an indistinguishable (4 pairs) or closely related PFGE pattern (3 pairs, defined as showing <3 fragment difference) (Figure). In addition, antimicrobial drug susceptibility patterns were similar, and only 1 variation was found in the virulence gene profiles (Table 1, Table 2). Five of these 7 patients reportedly had a profession where they worked with poultry. A total of 22 patients who did not share a clone of E. faecalis found in poultry in their household reported working with poultry.


Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam.

Poulsen LL, Bisgaard M, Son NT, Trung NV, An HM, Dalsgaard A - Emerging Infect. Dis. (2012)

Pulsed-field gel electrophoresis of SmaI-digested Enterococcus faecalis isolated from humans with urinary tract infections and from poultry in the same houselhold, Vietnam, January 2008–January 2010. Lanes A and P are molecular weight markers. Lane B, isolate 90U; lane C, isolate 90P; lane D, 122U; lane E, 122P; lane F, 186U; lane G, 186P; lane H, 191U; lane I, 191P; lane J, 204U; lane K, 204P; lane L, 217U; lane M, 217P; lane N, 221U; and lane O, 221P.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376801&req=5

Figure 1: Pulsed-field gel electrophoresis of SmaI-digested Enterococcus faecalis isolated from humans with urinary tract infections and from poultry in the same houselhold, Vietnam, January 2008–January 2010. Lanes A and P are molecular weight markers. Lane B, isolate 90U; lane C, isolate 90P; lane D, 122U; lane E, 122P; lane F, 186U; lane G, 186P; lane H, 191U; lane I, 191P; lane J, 204U; lane K, 204P; lane L, 217U; lane M, 217P; lane N, 221U; and lane O, 221P.
Mentions: In 7 (23%) of 31 UTI cases, E. faecalis isolated from patient urine and poultry demonstrated identical STs and an indistinguishable (4 pairs) or closely related PFGE pattern (3 pairs, defined as showing <3 fragment difference) (Figure). In addition, antimicrobial drug susceptibility patterns were similar, and only 1 variation was found in the virulence gene profiles (Table 1, Table 2). Five of these 7 patients reportedly had a profession where they worked with poultry. A total of 22 patients who did not share a clone of E. faecalis found in poultry in their household reported working with poultry.

Bottom Line: Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear.Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences.The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT
Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear. To investigate whether poultry might be a reservoir for E. faecalis-associated urinary tract infections (UTIs) in humans, we characterized E. faecalis isolates from patients in Vietnam with UTIs during January 2008-January 2010 and poultry living in close contact with them by multilocus sequence typing (MLST), pulsed-field gel electrophoresis, analysis of antimicrobial drug susceptibility patterns, and sequencing of virulence genes. In 7 (23%) of 31 UTI cases, we detected identical MLST, indistinguishable or closely related pulsed-field gel electrophoresis patterns, and similar antimicrobial drug susceptibility patterns. Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences. The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.

Show MeSH
Related in: MedlinePlus