Limits...
Dobrava hantavirus infection complicated by panhypopituitarism, Istanbul, Turkey, 2010.

Sarıgüzel N, Hofmann J, Canpolat AT, Türk A, Ettinger J, Atmaca D, Akyar I, Yücel S, Arıkan E, Uyar Y, Çağlayık DY, Kocagöz AS, Kaya A, Kruger DH - Emerging Infect. Dis. (2012)

Bottom Line: We identified Dobrava-Belgrade virus infection in Turkey (from a strain related to hantavirus strains from nearby countries) in a patient who had severe symptoms leading to panhypopituitarism, but no known risk for hantavirus.Our findings emphasize the need for increased awareness of hantaviruses in the region and assessment of symptomatic persons without known risk factors for infection.

View Article: PubMed Central - PubMed

Affiliation: Acıbadem Hospital, Istanbul, Turkey. sariguzel@hotmail.com

ABSTRACT
We identified Dobrava-Belgrade virus infection in Turkey (from a strain related to hantavirus strains from nearby countries) in a patient who had severe symptoms leading to panhypopituitarism, but no known risk for hantavirus. Our findings emphasize the need for increased awareness of hantaviruses in the region and assessment of symptomatic persons without known risk factors for infection.

Show MeSH

Related in: MedlinePlus

Molecular phylogenetic analysis of small (S) and medium (M) gene segments. Consensus neighbor-joining phylogenetic tree (Tamura-Nei 93 evolutionary model) of hantavirus strains was constructed as described (9) based on partial sequences of the S (panel A) and M segment (panel B). Bootstrap values >70%, calculated from 10,000 replicates, are shown at the tree branches. Sequences taken from GenBank are indicated by their accession numbers. SAAV Est, Saaremaa virus from Estonia; DOBV AnoP, Dobrava-Belgrade virus (lineage DOBV-Af) from Greece; DOBV Slo, Dobrava-Belgrade virus (lineage DOBV-Af) from Slovenia; DOB SK, Dobrava-Belgrade virus (lineage DOBV-Aa) from Slovakia; HTNV, Hantaan virus; SEOV, Seoul virus; ANDV, Andes virus; SNV, Sin nombre virus; PUUV, Puumala virus; TULV, Tula virus; PHV, Prospect Hill virus. Scale bars indicate an evolutionary distance of 0.1 substitutions per position.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3376800&req=5

Figure 1: Molecular phylogenetic analysis of small (S) and medium (M) gene segments. Consensus neighbor-joining phylogenetic tree (Tamura-Nei 93 evolutionary model) of hantavirus strains was constructed as described (9) based on partial sequences of the S (panel A) and M segment (panel B). Bootstrap values >70%, calculated from 10,000 replicates, are shown at the tree branches. Sequences taken from GenBank are indicated by their accession numbers. SAAV Est, Saaremaa virus from Estonia; DOBV AnoP, Dobrava-Belgrade virus (lineage DOBV-Af) from Greece; DOBV Slo, Dobrava-Belgrade virus (lineage DOBV-Af) from Slovenia; DOB SK, Dobrava-Belgrade virus (lineage DOBV-Aa) from Slovakia; HTNV, Hantaan virus; SEOV, Seoul virus; ANDV, Andes virus; SNV, Sin nombre virus; PUUV, Puumala virus; TULV, Tula virus; PHV, Prospect Hill virus. Scale bars indicate an evolutionary distance of 0.1 substitutions per position.

Mentions: Serologic testing for hantavirus was performed by using the recomLine Bunyavirus IgG/IgM immunoassay (Mikrogen, Neuried, Munich, Germany); results showed strong reactivity for IgG and IgM antibodies, indicating an acute hantavirus infection. The blot data provided evidence of an infection with DOBV; to confirm the results, we performed serotyping by using focus reduction neutralization tests (Table 2). The results confirmed a DOBV infection most likely caused by a strain of DOBV-Af. Reverse transcription PCR results for hantaviral RNA were positive for the first acute-phase blood sample. Subsequent nucleotide sequence determination of parts of the 3 genomic segments and molecular phylogenetic analysis of these small, medium (Figure 1), and large gene sequences (data not shown) showed that the isolate was most closely related to DOBV-Af.


Dobrava hantavirus infection complicated by panhypopituitarism, Istanbul, Turkey, 2010.

Sarıgüzel N, Hofmann J, Canpolat AT, Türk A, Ettinger J, Atmaca D, Akyar I, Yücel S, Arıkan E, Uyar Y, Çağlayık DY, Kocagöz AS, Kaya A, Kruger DH - Emerging Infect. Dis. (2012)

Molecular phylogenetic analysis of small (S) and medium (M) gene segments. Consensus neighbor-joining phylogenetic tree (Tamura-Nei 93 evolutionary model) of hantavirus strains was constructed as described (9) based on partial sequences of the S (panel A) and M segment (panel B). Bootstrap values >70%, calculated from 10,000 replicates, are shown at the tree branches. Sequences taken from GenBank are indicated by their accession numbers. SAAV Est, Saaremaa virus from Estonia; DOBV AnoP, Dobrava-Belgrade virus (lineage DOBV-Af) from Greece; DOBV Slo, Dobrava-Belgrade virus (lineage DOBV-Af) from Slovenia; DOB SK, Dobrava-Belgrade virus (lineage DOBV-Aa) from Slovakia; HTNV, Hantaan virus; SEOV, Seoul virus; ANDV, Andes virus; SNV, Sin nombre virus; PUUV, Puumala virus; TULV, Tula virus; PHV, Prospect Hill virus. Scale bars indicate an evolutionary distance of 0.1 substitutions per position.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376800&req=5

Figure 1: Molecular phylogenetic analysis of small (S) and medium (M) gene segments. Consensus neighbor-joining phylogenetic tree (Tamura-Nei 93 evolutionary model) of hantavirus strains was constructed as described (9) based on partial sequences of the S (panel A) and M segment (panel B). Bootstrap values >70%, calculated from 10,000 replicates, are shown at the tree branches. Sequences taken from GenBank are indicated by their accession numbers. SAAV Est, Saaremaa virus from Estonia; DOBV AnoP, Dobrava-Belgrade virus (lineage DOBV-Af) from Greece; DOBV Slo, Dobrava-Belgrade virus (lineage DOBV-Af) from Slovenia; DOB SK, Dobrava-Belgrade virus (lineage DOBV-Aa) from Slovakia; HTNV, Hantaan virus; SEOV, Seoul virus; ANDV, Andes virus; SNV, Sin nombre virus; PUUV, Puumala virus; TULV, Tula virus; PHV, Prospect Hill virus. Scale bars indicate an evolutionary distance of 0.1 substitutions per position.
Mentions: Serologic testing for hantavirus was performed by using the recomLine Bunyavirus IgG/IgM immunoassay (Mikrogen, Neuried, Munich, Germany); results showed strong reactivity for IgG and IgM antibodies, indicating an acute hantavirus infection. The blot data provided evidence of an infection with DOBV; to confirm the results, we performed serotyping by using focus reduction neutralization tests (Table 2). The results confirmed a DOBV infection most likely caused by a strain of DOBV-Af. Reverse transcription PCR results for hantaviral RNA were positive for the first acute-phase blood sample. Subsequent nucleotide sequence determination of parts of the 3 genomic segments and molecular phylogenetic analysis of these small, medium (Figure 1), and large gene sequences (data not shown) showed that the isolate was most closely related to DOBV-Af.

Bottom Line: We identified Dobrava-Belgrade virus infection in Turkey (from a strain related to hantavirus strains from nearby countries) in a patient who had severe symptoms leading to panhypopituitarism, but no known risk for hantavirus.Our findings emphasize the need for increased awareness of hantaviruses in the region and assessment of symptomatic persons without known risk factors for infection.

View Article: PubMed Central - PubMed

Affiliation: Acıbadem Hospital, Istanbul, Turkey. sariguzel@hotmail.com

ABSTRACT
We identified Dobrava-Belgrade virus infection in Turkey (from a strain related to hantavirus strains from nearby countries) in a patient who had severe symptoms leading to panhypopituitarism, but no known risk for hantavirus. Our findings emphasize the need for increased awareness of hantaviruses in the region and assessment of symptomatic persons without known risk factors for infection.

Show MeSH
Related in: MedlinePlus