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Outbreak-associated Vibrio cholerae genotypes with identical pulsotypes, Malaysia, 2009.

Teh CS, Suhaili Z, Lim KT, Khamaruddin MA, Yahya F, Sajili MH, Yeo CC, Thong KL - Emerging Infect. Dis. (2012)

Bottom Line: A cholera outbreak in Terengganu, Malaysia, in November 2009 was caused by 2 El Tor Vibrio cholerae variants resistant to typical antimicrobial drugs.Evidence of replacement of treatable V. cholerae infection in the region with antimicrobial-resistant strains calls for increased surveillance and prevention measures.

View Article: PubMed Central - PubMed

Affiliation: University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT
A cholera outbreak in Terengganu, Malaysia, in November 2009 was caused by 2 El Tor Vibrio cholerae variants resistant to typical antimicrobial drugs. Evidence of replacement of treatable V. cholerae infection in the region with antimicrobial-resistant strains calls for increased surveillance and prevention measures.

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Related in: MedlinePlus

NotI-pulsed-field gel electrophoresis profiles of Vibrio cholerae isolated during the outbreak, Terengganu, Malaysia, 2009. Lane M: XbaI-digested Salmonella enterica serovar Braenderup H9812 as DNA standard; lanes 1–7 and 9–12: isolates of El Tor O1 serogroup (rectal swab); lane 8: isolate of non–O1/non–O139 serogroup (swab from ice factory); lane 13: El Tor O1 V. cholerae isolated in 2008 (Kuala Lumpur).
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Figure 1: NotI-pulsed-field gel electrophoresis profiles of Vibrio cholerae isolated during the outbreak, Terengganu, Malaysia, 2009. Lane M: XbaI-digested Salmonella enterica serovar Braenderup H9812 as DNA standard; lanes 1–7 and 9–12: isolates of El Tor O1 serogroup (rectal swab); lane 8: isolate of non–O1/non–O139 serogroup (swab from ice factory); lane 13: El Tor O1 V. cholerae isolated in 2008 (Kuala Lumpur).

Mentions: PFGE of NotI-digested chromosomal DNA from all of the human isolates resulted in 1 pulsotype with 24 fragments (≈30 kb to ≈370 kb); the environmental isolate showed a distinct pulsotype (F = 0.83) (Figure 1). PFGE was repeated 2× with identical results. In addition, the pulsotype of the clinical isolates in this study was identical to the pulsotype of an O1 isolate (123/08) from a cholera patient in Kuala Lumpur in 2008 (Figure 1) (12). Isolate 123/08 also showed identical antibiograms with the Terengganu O1 outbreak isolates. This finding suggests that isolate 123/08 and the Terengganu 2009 O1 outbreak isolates were possibly linked.


Outbreak-associated Vibrio cholerae genotypes with identical pulsotypes, Malaysia, 2009.

Teh CS, Suhaili Z, Lim KT, Khamaruddin MA, Yahya F, Sajili MH, Yeo CC, Thong KL - Emerging Infect. Dis. (2012)

NotI-pulsed-field gel electrophoresis profiles of Vibrio cholerae isolated during the outbreak, Terengganu, Malaysia, 2009. Lane M: XbaI-digested Salmonella enterica serovar Braenderup H9812 as DNA standard; lanes 1–7 and 9–12: isolates of El Tor O1 serogroup (rectal swab); lane 8: isolate of non–O1/non–O139 serogroup (swab from ice factory); lane 13: El Tor O1 V. cholerae isolated in 2008 (Kuala Lumpur).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376796&req=5

Figure 1: NotI-pulsed-field gel electrophoresis profiles of Vibrio cholerae isolated during the outbreak, Terengganu, Malaysia, 2009. Lane M: XbaI-digested Salmonella enterica serovar Braenderup H9812 as DNA standard; lanes 1–7 and 9–12: isolates of El Tor O1 serogroup (rectal swab); lane 8: isolate of non–O1/non–O139 serogroup (swab from ice factory); lane 13: El Tor O1 V. cholerae isolated in 2008 (Kuala Lumpur).
Mentions: PFGE of NotI-digested chromosomal DNA from all of the human isolates resulted in 1 pulsotype with 24 fragments (≈30 kb to ≈370 kb); the environmental isolate showed a distinct pulsotype (F = 0.83) (Figure 1). PFGE was repeated 2× with identical results. In addition, the pulsotype of the clinical isolates in this study was identical to the pulsotype of an O1 isolate (123/08) from a cholera patient in Kuala Lumpur in 2008 (Figure 1) (12). Isolate 123/08 also showed identical antibiograms with the Terengganu O1 outbreak isolates. This finding suggests that isolate 123/08 and the Terengganu 2009 O1 outbreak isolates were possibly linked.

Bottom Line: A cholera outbreak in Terengganu, Malaysia, in November 2009 was caused by 2 El Tor Vibrio cholerae variants resistant to typical antimicrobial drugs.Evidence of replacement of treatable V. cholerae infection in the region with antimicrobial-resistant strains calls for increased surveillance and prevention measures.

View Article: PubMed Central - PubMed

Affiliation: University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT
A cholera outbreak in Terengganu, Malaysia, in November 2009 was caused by 2 El Tor Vibrio cholerae variants resistant to typical antimicrobial drugs. Evidence of replacement of treatable V. cholerae infection in the region with antimicrobial-resistant strains calls for increased surveillance and prevention measures.

Show MeSH
Related in: MedlinePlus