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Multiple introductions of avian influenza viruses (H5N1), Laos, 2009-2010.

Sonnberg S, Phommachanh P, Naipospos TS, McKenzie J, Chanthavisouk C, Pathammavong S, Darnell D, Meeduangchanh P, Rubrum AM, Souriya M, Khambounheuang B, Webby RJ, Douangngeun B, Webster RG - Emerging Infect. Dis. (2012)

Bottom Line: Avian influenza viruses (H5N1) of clades 2.3.4.1, 2.3.4.2, and 2.3.2.1 were introduced into Laos in 2009-2010.To investigate these viruses, we conducted active surveillance of poultry during March 2010.We detected viruses throughout Laos, including several interclade reassortants and 2 subgroups of clade 2.3.4, one of which caused an outbreak in May 2010.

View Article: PubMed Central - PubMed

Affiliation: Division of Virology, St Jude Children’s Research Hospital, Memphis, Tennessee 38105, USA. stephanie.sonnberg@stjude.org

ABSTRACT
Avian influenza viruses (H5N1) of clades 2.3.4.1, 2.3.4.2, and 2.3.2.1 were introduced into Laos in 2009-2010. To investigate these viruses, we conducted active surveillance of poultry during March 2010. We detected viruses throughout Laos, including several interclade reassortants and 2 subgroups of clade 2.3.4, one of which caused an outbreak in May 2010.

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Related in: MedlinePlus

Areas sampled and location of subtyped avian influenza viruses (H5N1), Laos, 2009–2010. Provinces that had previous outbreaks of highly pathogenic avian influenza and were part of the survey are indicated in gray, the province that had a previous outbreak but was not part of the survey is indicated in blue, and the province that had not had an outbreak but was part of the survey is indicated in green. Colored dots indicate presence of viruses: light blue, anti-H5 (clade 2.3.4); gray, anti-H9 lineage G1; red, clade 2.3.4.1; orange, clade 2.3.4.2; green, anti-H5 (clade 2.3.2); black, anti-H9 lineage Y280; white, anti-H4; yellow, anti-H6; purple, clade 2.3.2.1 or virus-specific antibodies.
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Figure 1: Areas sampled and location of subtyped avian influenza viruses (H5N1), Laos, 2009–2010. Provinces that had previous outbreaks of highly pathogenic avian influenza and were part of the survey are indicated in gray, the province that had a previous outbreak but was not part of the survey is indicated in blue, and the province that had not had an outbreak but was part of the survey is indicated in green. Colored dots indicate presence of viruses: light blue, anti-H5 (clade 2.3.4); gray, anti-H9 lineage G1; red, clade 2.3.4.1; orange, clade 2.3.4.2; green, anti-H5 (clade 2.3.2); black, anti-H9 lineage Y280; white, anti-H4; yellow, anti-H6; purple, clade 2.3.2.1 or virus-specific antibodies.

Mentions: Serum samples were collected in 9 of 17 provinces in Laos from healthy ducks and chickens in live-bird markets, village backyard flocks, and layer duck farms. Cloacal, tracheal, and environmental (fecal and water) swab specimens were also collected and placed immediately in transport medium (Figure). Swab specimens were screened in pools of 4 by using a real-time reverse transcription PCR for the matrix (M) gene segment (6). Positive pools were reextracted individually, retested, tested for hemagglutinin 5 (H5) by real-time reverse transcription PCR (7), and injected into 10–11-day-old embryonated chicken eggs.


Multiple introductions of avian influenza viruses (H5N1), Laos, 2009-2010.

Sonnberg S, Phommachanh P, Naipospos TS, McKenzie J, Chanthavisouk C, Pathammavong S, Darnell D, Meeduangchanh P, Rubrum AM, Souriya M, Khambounheuang B, Webby RJ, Douangngeun B, Webster RG - Emerging Infect. Dis. (2012)

Areas sampled and location of subtyped avian influenza viruses (H5N1), Laos, 2009–2010. Provinces that had previous outbreaks of highly pathogenic avian influenza and were part of the survey are indicated in gray, the province that had a previous outbreak but was not part of the survey is indicated in blue, and the province that had not had an outbreak but was part of the survey is indicated in green. Colored dots indicate presence of viruses: light blue, anti-H5 (clade 2.3.4); gray, anti-H9 lineage G1; red, clade 2.3.4.1; orange, clade 2.3.4.2; green, anti-H5 (clade 2.3.2); black, anti-H9 lineage Y280; white, anti-H4; yellow, anti-H6; purple, clade 2.3.2.1 or virus-specific antibodies.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376794&req=5

Figure 1: Areas sampled and location of subtyped avian influenza viruses (H5N1), Laos, 2009–2010. Provinces that had previous outbreaks of highly pathogenic avian influenza and were part of the survey are indicated in gray, the province that had a previous outbreak but was not part of the survey is indicated in blue, and the province that had not had an outbreak but was part of the survey is indicated in green. Colored dots indicate presence of viruses: light blue, anti-H5 (clade 2.3.4); gray, anti-H9 lineage G1; red, clade 2.3.4.1; orange, clade 2.3.4.2; green, anti-H5 (clade 2.3.2); black, anti-H9 lineage Y280; white, anti-H4; yellow, anti-H6; purple, clade 2.3.2.1 or virus-specific antibodies.
Mentions: Serum samples were collected in 9 of 17 provinces in Laos from healthy ducks and chickens in live-bird markets, village backyard flocks, and layer duck farms. Cloacal, tracheal, and environmental (fecal and water) swab specimens were also collected and placed immediately in transport medium (Figure). Swab specimens were screened in pools of 4 by using a real-time reverse transcription PCR for the matrix (M) gene segment (6). Positive pools were reextracted individually, retested, tested for hemagglutinin 5 (H5) by real-time reverse transcription PCR (7), and injected into 10–11-day-old embryonated chicken eggs.

Bottom Line: Avian influenza viruses (H5N1) of clades 2.3.4.1, 2.3.4.2, and 2.3.2.1 were introduced into Laos in 2009-2010.To investigate these viruses, we conducted active surveillance of poultry during March 2010.We detected viruses throughout Laos, including several interclade reassortants and 2 subgroups of clade 2.3.4, one of which caused an outbreak in May 2010.

View Article: PubMed Central - PubMed

Affiliation: Division of Virology, St Jude Children’s Research Hospital, Memphis, Tennessee 38105, USA. stephanie.sonnberg@stjude.org

ABSTRACT
Avian influenza viruses (H5N1) of clades 2.3.4.1, 2.3.4.2, and 2.3.2.1 were introduced into Laos in 2009-2010. To investigate these viruses, we conducted active surveillance of poultry during March 2010. We detected viruses throughout Laos, including several interclade reassortants and 2 subgroups of clade 2.3.4, one of which caused an outbreak in May 2010.

Show MeSH
Related in: MedlinePlus