Limits...
Electrochemical oxidation of cysteine at a film gold modified carbon fiber microelectrode its application in a flow-through voltammetric sensor.

Wang LH, Huang WS - Sensors (Basel) (2012)

Bottom Line: The possible reaction mechanism of the oxidation process is described from the relations to scan rate, peak potentials and currents.For the pulse mode, and measurements with suitable experimental parameters, a linear concentration from 0.5 to 5.0 mg·L(-1) was found.The limit of quantification for cysteine was below 60 ng·mL(-1).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Chemistry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan. e201466.wang@msa.hinet.net

ABSTRACT
A flow-electrolytical cell containing a strand of micro Au modified carbon fiber electrodes (CFE) has been designedand characterized for use in a voltammatric detector for detecting cysteine using high-performance liquid chromatography. Cysteine is more efficiently electrochemical oxidized on a Au /CFE than a bare gold and carbon fiber electrode. The possible reaction mechanism of the oxidation process is described from the relations to scan rate, peak potentials and currents. For the pulse mode, and measurements with suitable experimental parameters, a linear concentration from 0.5 to 5.0 mg·L(-1) was found. The limit of quantification for cysteine was below 60 ng·mL(-1).

Show MeSH
The LC-ECD chromatograms recorded to produce analytical curves for cysteine. Peaks; (a) 1.0 mg·L−1; (b) 2.0 mg·L−1; (c) 3.0 mg·L−1; (d) 4.0 mg·L−1. Liquid chromatography-electrochemical detection analysis conditions were identical to those listed in Figure 12.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3376634&req=5

f14-sensors-12-03562: The LC-ECD chromatograms recorded to produce analytical curves for cysteine. Peaks; (a) 1.0 mg·L−1; (b) 2.0 mg·L−1; (c) 3.0 mg·L−1; (d) 4.0 mg·L−1. Liquid chromatography-electrochemical detection analysis conditions were identical to those listed in Figure 12.

Mentions: The proposed LC-PAD method was applied to the determination of cysteine and the resulting chromatograms are shown in Figure 14. The calibration curve showed good linearity over the range 0.5–4.0 mg·L−1; the regression equation was y =169 x + 24.4, and the correlation coefficient was r = 0.9984. The limits of quantification for cysteine was below 60 ng·m·L−1.


Electrochemical oxidation of cysteine at a film gold modified carbon fiber microelectrode its application in a flow-through voltammetric sensor.

Wang LH, Huang WS - Sensors (Basel) (2012)

The LC-ECD chromatograms recorded to produce analytical curves for cysteine. Peaks; (a) 1.0 mg·L−1; (b) 2.0 mg·L−1; (c) 3.0 mg·L−1; (d) 4.0 mg·L−1. Liquid chromatography-electrochemical detection analysis conditions were identical to those listed in Figure 12.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376634&req=5

f14-sensors-12-03562: The LC-ECD chromatograms recorded to produce analytical curves for cysteine. Peaks; (a) 1.0 mg·L−1; (b) 2.0 mg·L−1; (c) 3.0 mg·L−1; (d) 4.0 mg·L−1. Liquid chromatography-electrochemical detection analysis conditions were identical to those listed in Figure 12.
Mentions: The proposed LC-PAD method was applied to the determination of cysteine and the resulting chromatograms are shown in Figure 14. The calibration curve showed good linearity over the range 0.5–4.0 mg·L−1; the regression equation was y =169 x + 24.4, and the correlation coefficient was r = 0.9984. The limits of quantification for cysteine was below 60 ng·m·L−1.

Bottom Line: The possible reaction mechanism of the oxidation process is described from the relations to scan rate, peak potentials and currents.For the pulse mode, and measurements with suitable experimental parameters, a linear concentration from 0.5 to 5.0 mg·L(-1) was found.The limit of quantification for cysteine was below 60 ng·mL(-1).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Chemistry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan. e201466.wang@msa.hinet.net

ABSTRACT
A flow-electrolytical cell containing a strand of micro Au modified carbon fiber electrodes (CFE) has been designedand characterized for use in a voltammatric detector for detecting cysteine using high-performance liquid chromatography. Cysteine is more efficiently electrochemical oxidized on a Au /CFE than a bare gold and carbon fiber electrode. The possible reaction mechanism of the oxidation process is described from the relations to scan rate, peak potentials and currents. For the pulse mode, and measurements with suitable experimental parameters, a linear concentration from 0.5 to 5.0 mg·L(-1) was found. The limit of quantification for cysteine was below 60 ng·mL(-1).

Show MeSH