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Cell-based sensor system using L6 cells for broad band continuous pollutant monitoring in aquatic environments.

Kubisch R, Bohrn U, Fleischer M, Stütz E - Sensors (Basel) (2012)

Bottom Line: In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown.A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts).In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Biology-Biotechnology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilian-University Munich, Munich, Germany. rebekka.kubisch@cup.uni-muenchen.de

ABSTRACT
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen consumption (respiration) and impedance (morphology) of the cells. A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts). The cytotoxicity or cellular effects induced by inorganic ions (Ni(2+) and Cu(2+)) can be detected with the metabolic parameters acidification and respiration down to 0.5 mg/L, whereas the detection limit for other substances like nicotine and acetaminophen are rather high, in the range of 0.1 mg/L and 100 mg/L. In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances. The results support the paradigm change from single substance detection to the monitoring of overall toxicity.

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Related in: MedlinePlus

Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with NiCl2 in the Bionas 2500 Analyzing System. Vehicle control (black), 50 mg/L (red), 25 mg/L (blue), 5 mg/L (green), 2.5 mg/L (yellow) and 0.5 mg/L (grey). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nickel chloride exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nickel chloride for 24 h.
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f4-sensors-12-03370: Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with NiCl2 in the Bionas 2500 Analyzing System. Vehicle control (black), 50 mg/L (red), 25 mg/L (blue), 5 mg/L (green), 2.5 mg/L (yellow) and 0.5 mg/L (grey). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nickel chloride exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nickel chloride for 24 h.

Mentions: In the Bionas analyzing system, the presence of nickel ions results in a decrease of impedance signals of L6-cells down to almost 80% at concentrations of 25 and 50 mg/L (see Figure 4(a)) which might arise from the membrane disruptive effects of nickel ions. The acidification is immediately impaired at the beginning of the incubation with Ni2+-containing media solution (see Figure 4(b)). If Ni2+ ions are removed cells are able to recover from this impact within three hours also after exposure to 50 mg/L Ni2+. A low nickel concentration of 0.5 mg/L seems to slightly activate the cellular metabolism, indicated in an increase of extracellular acidification (see Figure 4(d)). Similar to the acidification, the cellular respiration is inhibited only at high Ni2+ concentrations (25 and 50 mg/L; see Figure 4(c)).


Cell-based sensor system using L6 cells for broad band continuous pollutant monitoring in aquatic environments.

Kubisch R, Bohrn U, Fleischer M, Stütz E - Sensors (Basel) (2012)

Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with NiCl2 in the Bionas 2500 Analyzing System. Vehicle control (black), 50 mg/L (red), 25 mg/L (blue), 5 mg/L (green), 2.5 mg/L (yellow) and 0.5 mg/L (grey). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nickel chloride exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nickel chloride for 24 h.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376625&req=5

f4-sensors-12-03370: Time course of the impedance (a), acidification (b) and respiration (c) of L6 rat skeletal muscle cells during 24 h incubation with NiCl2 in the Bionas 2500 Analyzing System. Vehicle control (black), 50 mg/L (red), 25 mg/L (blue), 5 mg/L (green), 2.5 mg/L (yellow) and 0.5 mg/L (grey). Phases with running medium (RM) represent culture medium treatment without added substances. (d) Percent of control of impedance, respiration and acidification endpoint values measured after 15 h of nickel chloride exposure (n = 3). (e) Results of BrdU assay of L6 cells incubated with nickel chloride for 24 h.
Mentions: In the Bionas analyzing system, the presence of nickel ions results in a decrease of impedance signals of L6-cells down to almost 80% at concentrations of 25 and 50 mg/L (see Figure 4(a)) which might arise from the membrane disruptive effects of nickel ions. The acidification is immediately impaired at the beginning of the incubation with Ni2+-containing media solution (see Figure 4(b)). If Ni2+ ions are removed cells are able to recover from this impact within three hours also after exposure to 50 mg/L Ni2+. A low nickel concentration of 0.5 mg/L seems to slightly activate the cellular metabolism, indicated in an increase of extracellular acidification (see Figure 4(d)). Similar to the acidification, the cellular respiration is inhibited only at high Ni2+ concentrations (25 and 50 mg/L; see Figure 4(c)).

Bottom Line: In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown.A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts).In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Biology-Biotechnology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilian-University Munich, Munich, Germany. rebekka.kubisch@cup.uni-muenchen.de

ABSTRACT
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen consumption (respiration) and impedance (morphology) of the cells. A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts). The cytotoxicity or cellular effects induced by inorganic ions (Ni(2+) and Cu(2+)) can be detected with the metabolic parameters acidification and respiration down to 0.5 mg/L, whereas the detection limit for other substances like nicotine and acetaminophen are rather high, in the range of 0.1 mg/L and 100 mg/L. In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances. The results support the paradigm change from single substance detection to the monitoring of overall toxicity.

Show MeSH
Related in: MedlinePlus