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Cell-based sensor system using L6 cells for broad band continuous pollutant monitoring in aquatic environments.

Kubisch R, Bohrn U, Fleischer M, Stütz E - Sensors (Basel) (2012)

Bottom Line: In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown.A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts).In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Biology-Biotechnology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilian-University Munich, Munich, Germany. rebekka.kubisch@cup.uni-muenchen.de

ABSTRACT
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen consumption (respiration) and impedance (morphology) of the cells. A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts). The cytotoxicity or cellular effects induced by inorganic ions (Ni(2+) and Cu(2+)) can be detected with the metabolic parameters acidification and respiration down to 0.5 mg/L, whereas the detection limit for other substances like nicotine and acetaminophen are rather high, in the range of 0.1 mg/L and 100 mg/L. In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances. The results support the paradigm change from single substance detection to the monitoring of overall toxicity.

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Related in: MedlinePlus

Time course of impedance (a), acidification (b) and respiration (c) of different eukaryotic cell types during 18 h incubation in the Bionas 2500 Analyzing System: human colon carcinoma cell line HT-29 (black), Canine hepatocytes (red), hepatocellular carcinoma cell line HepG2 (blue), rat skeletal muscle cell line L6 (green), normal human dermal fibroblasts NHDF (yellow) and Chinese hamster lung fibroblasts V79 cells (grey).
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f3-sensors-12-03370: Time course of impedance (a), acidification (b) and respiration (c) of different eukaryotic cell types during 18 h incubation in the Bionas 2500 Analyzing System: human colon carcinoma cell line HT-29 (black), Canine hepatocytes (red), hepatocellular carcinoma cell line HepG2 (blue), rat skeletal muscle cell line L6 (green), normal human dermal fibroblasts NHDF (yellow) and Chinese hamster lung fibroblasts V79 cells (grey).

Mentions: Figure 3(a–c) show the response profiles of the tested cell types summarized in Table 2. Changes in the adhesion properties or in the cellular morphology assessed by the impedance measurement do not occur over at least 18 h of cultivation in any of the cells (Figure 3(a)).


Cell-based sensor system using L6 cells for broad band continuous pollutant monitoring in aquatic environments.

Kubisch R, Bohrn U, Fleischer M, Stütz E - Sensors (Basel) (2012)

Time course of impedance (a), acidification (b) and respiration (c) of different eukaryotic cell types during 18 h incubation in the Bionas 2500 Analyzing System: human colon carcinoma cell line HT-29 (black), Canine hepatocytes (red), hepatocellular carcinoma cell line HepG2 (blue), rat skeletal muscle cell line L6 (green), normal human dermal fibroblasts NHDF (yellow) and Chinese hamster lung fibroblasts V79 cells (grey).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376625&req=5

f3-sensors-12-03370: Time course of impedance (a), acidification (b) and respiration (c) of different eukaryotic cell types during 18 h incubation in the Bionas 2500 Analyzing System: human colon carcinoma cell line HT-29 (black), Canine hepatocytes (red), hepatocellular carcinoma cell line HepG2 (blue), rat skeletal muscle cell line L6 (green), normal human dermal fibroblasts NHDF (yellow) and Chinese hamster lung fibroblasts V79 cells (grey).
Mentions: Figure 3(a–c) show the response profiles of the tested cell types summarized in Table 2. Changes in the adhesion properties or in the cellular morphology assessed by the impedance measurement do not occur over at least 18 h of cultivation in any of the cells (Figure 3(a)).

Bottom Line: In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown.A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts).In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Biology-Biotechnology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilian-University Munich, Munich, Germany. rebekka.kubisch@cup.uni-muenchen.de

ABSTRACT
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen consumption (respiration) and impedance (morphology) of the cells. A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts). The cytotoxicity or cellular effects induced by inorganic ions (Ni(2+) and Cu(2+)) can be detected with the metabolic parameters acidification and respiration down to 0.5 mg/L, whereas the detection limit for other substances like nicotine and acetaminophen are rather high, in the range of 0.1 mg/L and 100 mg/L. In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances. The results support the paradigm change from single substance detection to the monitoring of overall toxicity.

Show MeSH
Related in: MedlinePlus