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Cell-based sensor system using L6 cells for broad band continuous pollutant monitoring in aquatic environments.

Kubisch R, Bohrn U, Fleischer M, Stütz E - Sensors (Basel) (2012)

Bottom Line: In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown.A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts).In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Biology-Biotechnology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilian-University Munich, Munich, Germany. rebekka.kubisch@cup.uni-muenchen.de

ABSTRACT
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen consumption (respiration) and impedance (morphology) of the cells. A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts). The cytotoxicity or cellular effects induced by inorganic ions (Ni(2+) and Cu(2+)) can be detected with the metabolic parameters acidification and respiration down to 0.5 mg/L, whereas the detection limit for other substances like nicotine and acetaminophen are rather high, in the range of 0.1 mg/L and 100 mg/L. In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances. The results support the paradigm change from single substance detection to the monitoring of overall toxicity.

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Related in: MedlinePlus

Adherent monolayer of L6 (rat skeletal muscle cells) cells on a Bionas SC 1000 Metabolic Chip.
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f1-sensors-12-03370: Adherent monolayer of L6 (rat skeletal muscle cells) cells on a Bionas SC 1000 Metabolic Chip.

Mentions: The system has been described in detail elsewhere [46]. In brief, the experimental setup was as follows: depending on the size of the cells, (2–10) × 105 cells per chip (SC 1000 Metabolic chip, Bionas, see Figure 1) were seeded 1 day before the start of the experiment and cultured in growth media in a 5% CO2 atmosphere at 37 °C under saturated humidity to form a confluent cell monolayer within 24 h. The growth area on the chip which is defined by the chip housing is 70.88 mm2. One hour prior to inserting the chips into the Bionas System device, growth medium on the chips was replaced by carbonate buffer-free running medium containing 1 mM Hepes (BioWhittaker) instead and reduced (1%) serum concentration.


Cell-based sensor system using L6 cells for broad band continuous pollutant monitoring in aquatic environments.

Kubisch R, Bohrn U, Fleischer M, Stütz E - Sensors (Basel) (2012)

Adherent monolayer of L6 (rat skeletal muscle cells) cells on a Bionas SC 1000 Metabolic Chip.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376625&req=5

f1-sensors-12-03370: Adherent monolayer of L6 (rat skeletal muscle cells) cells on a Bionas SC 1000 Metabolic Chip.
Mentions: The system has been described in detail elsewhere [46]. In brief, the experimental setup was as follows: depending on the size of the cells, (2–10) × 105 cells per chip (SC 1000 Metabolic chip, Bionas, see Figure 1) were seeded 1 day before the start of the experiment and cultured in growth media in a 5% CO2 atmosphere at 37 °C under saturated humidity to form a confluent cell monolayer within 24 h. The growth area on the chip which is defined by the chip housing is 70.88 mm2. One hour prior to inserting the chips into the Bionas System device, growth medium on the chips was replaced by carbonate buffer-free running medium containing 1 mM Hepes (BioWhittaker) instead and reduced (1%) serum concentration.

Bottom Line: In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown.A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts).In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Biology-Biotechnology, Department of Pharmacy, Center for Drug Research, Ludwig-Maximilian-University Munich, Munich, Germany. rebekka.kubisch@cup.uni-muenchen.de

ABSTRACT
Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are urgently needed. In this study, the applicability of a cell-based sensor system using selected eukaryotic cell lines for the detection of aquatic pollutants is shown. Readout parameters of the cells were the acidification (metabolism), oxygen consumption (respiration) and impedance (morphology) of the cells. A variety of potential cytotoxic classes of substances (heavy metals, pharmaceuticals, neurotoxins, waste water) was tested with monolayers of L6 cells (rat myoblasts). The cytotoxicity or cellular effects induced by inorganic ions (Ni(2+) and Cu(2+)) can be detected with the metabolic parameters acidification and respiration down to 0.5 mg/L, whereas the detection limit for other substances like nicotine and acetaminophen are rather high, in the range of 0.1 mg/L and 100 mg/L. In a close to application model a real waste water sample shows detectable signals, indicating the existence of cytotoxic substances. The results support the paradigm change from single substance detection to the monitoring of overall toxicity.

Show MeSH
Related in: MedlinePlus