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An organic/inorganic hybrid membrane as a solid "turn-on" fluorescent chemosensor for coenzyme A (CoA), cysteine (Cys), and glutathione (GSH) in aqueous media.

Vallejos S, Estévez P, Ibeas S, García FC, Serna F, García JM - Sensors (Basel) (2012)

Bottom Line: In this way, the water insoluble sensing motif can be exploited in aqueous media.The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II).The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Burgos, Spain. svallejos@ubu.es

ABSTRACT
The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water "dip-in" fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a "turn-on" pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

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Selected fluorescence spectra (left) and titration curve (right) of M2 upon adding increasing quantities of CoA in water at physiological pH (pH = 7.4, TRIS) at an excitation wavelength of 400 nm. The inset for the figure on the right is an expansion of the lower concentrations of the titration curve.
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f4-sensors-12-02969: Selected fluorescence spectra (left) and titration curve (right) of M2 upon adding increasing quantities of CoA in water at physiological pH (pH = 7.4, TRIS) at an excitation wavelength of 400 nm. The inset for the figure on the right is an expansion of the lower concentrations of the titration curve.

Mentions: The membrane M1 behaves as a sensory material for the fluorogenic detection of Hg(II) in aqueous media. Upon the addition of Hg(II), the fluorescence of the membrane at 548 nm was quenched, which demonstrated that the membrane had “turn-off” fluorescence behaviour in the presence of the cation. This observation was attributed to the interaction of the Hg(II) with the N-terminus of the sensory motif (3) within the membrane at a 1:1 stoichiometry [31]. The integral preparation of a membrane containing equal molar quantities of (3) and Hg(II) (i.e., M2), led to a material with a partially quenched fluorescence. Moreover, fluorescence recovery was observed for M2 upon adding different biomolecules, e.g., CoA, Cys and GSH (see Figure 4). The stronger interaction of these biomolecules with Hg(II) led to a fluorescent chemosensor with fluorescence “turn-on” behaviour, based on the displacement approach [1,2]. A titration curve of the biomolecules was obtained by plotting the fluorescence maxima versus the biomolecule concentration. An illustrative example is shown for CoA in Figure 4. The limit of detection (LOD) was approximately 2 × 10−10 M.


An organic/inorganic hybrid membrane as a solid "turn-on" fluorescent chemosensor for coenzyme A (CoA), cysteine (Cys), and glutathione (GSH) in aqueous media.

Vallejos S, Estévez P, Ibeas S, García FC, Serna F, García JM - Sensors (Basel) (2012)

Selected fluorescence spectra (left) and titration curve (right) of M2 upon adding increasing quantities of CoA in water at physiological pH (pH = 7.4, TRIS) at an excitation wavelength of 400 nm. The inset for the figure on the right is an expansion of the lower concentrations of the titration curve.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376624&req=5

f4-sensors-12-02969: Selected fluorescence spectra (left) and titration curve (right) of M2 upon adding increasing quantities of CoA in water at physiological pH (pH = 7.4, TRIS) at an excitation wavelength of 400 nm. The inset for the figure on the right is an expansion of the lower concentrations of the titration curve.
Mentions: The membrane M1 behaves as a sensory material for the fluorogenic detection of Hg(II) in aqueous media. Upon the addition of Hg(II), the fluorescence of the membrane at 548 nm was quenched, which demonstrated that the membrane had “turn-off” fluorescence behaviour in the presence of the cation. This observation was attributed to the interaction of the Hg(II) with the N-terminus of the sensory motif (3) within the membrane at a 1:1 stoichiometry [31]. The integral preparation of a membrane containing equal molar quantities of (3) and Hg(II) (i.e., M2), led to a material with a partially quenched fluorescence. Moreover, fluorescence recovery was observed for M2 upon adding different biomolecules, e.g., CoA, Cys and GSH (see Figure 4). The stronger interaction of these biomolecules with Hg(II) led to a fluorescent chemosensor with fluorescence “turn-on” behaviour, based on the displacement approach [1,2]. A titration curve of the biomolecules was obtained by plotting the fluorescence maxima versus the biomolecule concentration. An illustrative example is shown for CoA in Figure 4. The limit of detection (LOD) was approximately 2 × 10−10 M.

Bottom Line: In this way, the water insoluble sensing motif can be exploited in aqueous media.The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II).The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Burgos, Spain. svallejos@ubu.es

ABSTRACT
The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water "dip-in" fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a "turn-on" pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

Show MeSH
Related in: MedlinePlus