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An organic/inorganic hybrid membrane as a solid "turn-on" fluorescent chemosensor for coenzyme A (CoA), cysteine (Cys), and glutathione (GSH) in aqueous media.

Vallejos S, Estévez P, Ibeas S, García FC, Serna F, García JM - Sensors (Basel) (2012)

Bottom Line: In this way, the water insoluble sensing motif can be exploited in aqueous media.The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II).The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Burgos, Spain. svallejos@ubu.es

ABSTRACT
The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water "dip-in" fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a "turn-on" pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

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FTIR spectra of the dried membranes M1 (black line) and M2 (red line).
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f2-sensors-12-02969: FTIR spectra of the dried membranes M1 (black line) and M2 (red line).

Mentions: The polymerisation of the comonomers without and with low molar content mercury (II) acetate (0.25%) (M1 and M2, respectively) resulted in materials with fairly different FTIR spectra (Figure 2). Comparing the spectra of dry samples of M1 and M2 resulted in the observation of an intense band that developed at 1,722 cm−1 for M2, with a concomitant shift of the amide I band to higher energies, from 1,668 to 1,675 cm−1, probably due to the acetate group.


An organic/inorganic hybrid membrane as a solid "turn-on" fluorescent chemosensor for coenzyme A (CoA), cysteine (Cys), and glutathione (GSH) in aqueous media.

Vallejos S, Estévez P, Ibeas S, García FC, Serna F, García JM - Sensors (Basel) (2012)

FTIR spectra of the dried membranes M1 (black line) and M2 (red line).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376624&req=5

f2-sensors-12-02969: FTIR spectra of the dried membranes M1 (black line) and M2 (red line).
Mentions: The polymerisation of the comonomers without and with low molar content mercury (II) acetate (0.25%) (M1 and M2, respectively) resulted in materials with fairly different FTIR spectra (Figure 2). Comparing the spectra of dry samples of M1 and M2 resulted in the observation of an intense band that developed at 1,722 cm−1 for M2, with a concomitant shift of the amide I band to higher energies, from 1,668 to 1,675 cm−1, probably due to the acetate group.

Bottom Line: In this way, the water insoluble sensing motif can be exploited in aqueous media.The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II).The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Burgos, Spain. svallejos@ubu.es

ABSTRACT
The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water "dip-in" fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a "turn-on" pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.

Show MeSH
Related in: MedlinePlus