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Electrochemical L-lactic acid sensor based on immobilized ZnO nanorods with lactate oxidase.

Ibupoto ZH, Shah SM, Khun K, Willander M - Sensors (Basel) (2012)

Bottom Line: The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor.We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade.The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

View Article: PubMed Central - PubMed

Affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping, Sweden. zafar.hussain.ibupoto@liu.se

ABSTRACT
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

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The response time of the proposed biosensor.
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f5-sensors-12-02456: The response time of the proposed biosensor.

Mentions: The calculated selectivity coefficient (K) values for these interfering substances are given in Table 1. It has been seen already that ascorbic acid has a significant effect on the response of lactic acid biosensors [53], but the present biosensor has shown no response to ascorbic acid. We used the 1 × 10−3 mM concentration range of each interfering substance. It has been observed that the proposed biosensor has the ability to work very well against the common interfering substances and therefore can be applied to determine the l-lactic acid in biological fluids, food, sport and clinical samples. Beside the selectivity, we examined the response time of the biosensor for all the detectable concentration ranges. It was observed that biosensor showed fast response times for higher concentrations and slower response times for low concentrations. Thus, the biosensor has shown a response time less than 10 s for all concentrations ranging from 0.01 mM to 1 mM, as shown in Figure 5.


Electrochemical L-lactic acid sensor based on immobilized ZnO nanorods with lactate oxidase.

Ibupoto ZH, Shah SM, Khun K, Willander M - Sensors (Basel) (2012)

The response time of the proposed biosensor.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376621&req=5

f5-sensors-12-02456: The response time of the proposed biosensor.
Mentions: The calculated selectivity coefficient (K) values for these interfering substances are given in Table 1. It has been seen already that ascorbic acid has a significant effect on the response of lactic acid biosensors [53], but the present biosensor has shown no response to ascorbic acid. We used the 1 × 10−3 mM concentration range of each interfering substance. It has been observed that the proposed biosensor has the ability to work very well against the common interfering substances and therefore can be applied to determine the l-lactic acid in biological fluids, food, sport and clinical samples. Beside the selectivity, we examined the response time of the biosensor for all the detectable concentration ranges. It was observed that biosensor showed fast response times for higher concentrations and slower response times for low concentrations. Thus, the biosensor has shown a response time less than 10 s for all concentrations ranging from 0.01 mM to 1 mM, as shown in Figure 5.

Bottom Line: The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor.We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade.The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

View Article: PubMed Central - PubMed

Affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping, Sweden. zafar.hussain.ibupoto@liu.se

ABSTRACT
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

Show MeSH