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Electrochemical L-lactic acid sensor based on immobilized ZnO nanorods with lactate oxidase.

Ibupoto ZH, Shah SM, Khun K, Willander M - Sensors (Basel) (2012)

Bottom Line: The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor.We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade.The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

View Article: PubMed Central - PubMed

Affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping, Sweden. zafar.hussain.ibupoto@liu.se

ABSTRACT
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

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Calibration curve of lactate oxidase immobilized ZnO nanorods biosensor for l-lactic acid concentration 1 × 10−4 to 1 × 10 0 mM.
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f2-sensors-12-02456: Calibration curve of lactate oxidase immobilized ZnO nanorods biosensor for l-lactic acid concentration 1 × 10−4 to 1 × 10 0 mM.

Mentions: The electrochemical response (EMF) of our proposed l-lactic acid biosensor based on lactate oxidase immobilized on the surface of ZnO nanorods in test electrolyte solution is shown in Figure 2.


Electrochemical L-lactic acid sensor based on immobilized ZnO nanorods with lactate oxidase.

Ibupoto ZH, Shah SM, Khun K, Willander M - Sensors (Basel) (2012)

Calibration curve of lactate oxidase immobilized ZnO nanorods biosensor for l-lactic acid concentration 1 × 10−4 to 1 × 10 0 mM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376621&req=5

f2-sensors-12-02456: Calibration curve of lactate oxidase immobilized ZnO nanorods biosensor for l-lactic acid concentration 1 × 10−4 to 1 × 10 0 mM.
Mentions: The electrochemical response (EMF) of our proposed l-lactic acid biosensor based on lactate oxidase immobilized on the surface of ZnO nanorods in test electrolyte solution is shown in Figure 2.

Bottom Line: The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor.We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade.The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

View Article: PubMed Central - PubMed

Affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping, Sweden. zafar.hussain.ibupoto@liu.se

ABSTRACT
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10(-4)-1 × 10(0) mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

Show MeSH