Limits...
N-acyl homoserine lactone production by Klebsiella pneumoniae isolated from human tongue surface.

Yin WF, Purmal K, Chin S, Chan XY, Koh CL, Sam CK, Chan KG - Sensors (Basel) (2012)

Bottom Line: Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual.Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs.High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com

ABSTRACT
Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium.

Show MeSH

Related in: MedlinePlus

Measurement of bioluminescence. Bacterial bioluminescence and OD were measured throughout growth for 24 hours at 37 °C in the presence of synthetic C4-HSL (square) (positive control), ethyl acetate (diamond) (negative control), and AHL extracts from T2-1-1 (K. pneumoniae) (asterisk) and T2-1-2 (K. pneumoniae) (circle). E. coli [pSB401] served as the biosensor. Data represent means from three replicates.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3376583&req=5

f4-sensors-12-03472: Measurement of bioluminescence. Bacterial bioluminescence and OD were measured throughout growth for 24 hours at 37 °C in the presence of synthetic C4-HSL (square) (positive control), ethyl acetate (diamond) (negative control), and AHL extracts from T2-1-1 (K. pneumoniae) (asterisk) and T2-1-2 (K. pneumoniae) (circle). E. coli [pSB401] served as the biosensor. Data represent means from three replicates.

Mentions: The production of AHLs by T2-1-1 and T2-1-2 was confirmed by the activation of bioluminescence of E. coli [pSB401] (Figure 4). However, the light emission was delayed in the presence of AHL extracts from strains T2-1-1 and T2-1-2 as compared to the positive control (Figure 4). This is because in the positive control, C4-HSL was added exogenously in a sufficient amount (100 mM) that triggered bioluminescence earlier.


N-acyl homoserine lactone production by Klebsiella pneumoniae isolated from human tongue surface.

Yin WF, Purmal K, Chin S, Chan XY, Koh CL, Sam CK, Chan KG - Sensors (Basel) (2012)

Measurement of bioluminescence. Bacterial bioluminescence and OD were measured throughout growth for 24 hours at 37 °C in the presence of synthetic C4-HSL (square) (positive control), ethyl acetate (diamond) (negative control), and AHL extracts from T2-1-1 (K. pneumoniae) (asterisk) and T2-1-2 (K. pneumoniae) (circle). E. coli [pSB401] served as the biosensor. Data represent means from three replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376583&req=5

f4-sensors-12-03472: Measurement of bioluminescence. Bacterial bioluminescence and OD were measured throughout growth for 24 hours at 37 °C in the presence of synthetic C4-HSL (square) (positive control), ethyl acetate (diamond) (negative control), and AHL extracts from T2-1-1 (K. pneumoniae) (asterisk) and T2-1-2 (K. pneumoniae) (circle). E. coli [pSB401] served as the biosensor. Data represent means from three replicates.
Mentions: The production of AHLs by T2-1-1 and T2-1-2 was confirmed by the activation of bioluminescence of E. coli [pSB401] (Figure 4). However, the light emission was delayed in the presence of AHL extracts from strains T2-1-1 and T2-1-2 as compared to the positive control (Figure 4). This is because in the positive control, C4-HSL was added exogenously in a sufficient amount (100 mM) that triggered bioluminescence earlier.

Bottom Line: Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual.Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs.High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com

ABSTRACT
Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium.

Show MeSH
Related in: MedlinePlus