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N-acyl homoserine lactone production by Klebsiella pneumoniae isolated from human tongue surface.

Yin WF, Purmal K, Chin S, Chan XY, Koh CL, Sam CK, Chan KG - Sensors (Basel) (2012)

Bottom Line: Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual.Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs.High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com

ABSTRACT
Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium.

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TLC analysis of AHL production by oral K. pneumoniae strains. Arrows indicate the positions of AHL standards run on the same plate. Lane A: C6-HSL (C6) and C8-HSL (C8). Lane B: 3-oxo-C6-HSL (OC6), C8-HSL (C8), and C10-HSL (C10). Lanes C and D: Extracts of spent culture supernatants from T2-1-1 and T2-1-2, respectively.
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f3-sensors-12-03472: TLC analysis of AHL production by oral K. pneumoniae strains. Arrows indicate the positions of AHL standards run on the same plate. Lane A: C6-HSL (C6) and C8-HSL (C8). Lane B: 3-oxo-C6-HSL (OC6), C8-HSL (C8), and C10-HSL (C10). Lanes C and D: Extracts of spent culture supernatants from T2-1-1 and T2-1-2, respectively.

Mentions: We succeeded in our initial objective of isolating quorum quenching bacteria from the human tongue surface (data not shown). We then tested these isolates for AHL production. To our surprise, isolates T2-1-1 and T2-1-2 produced AHLs, detected initially with A. tumefaciens NTL4(pZLR4) on agar plates–(data not shown). Subsequently, TLC results of the extracts of spent culture supernatants from T2-1-1 and T2-1-2 revealed three well-resolved spots with relative migration factor (Rf) values corresponding to those of 3-oxo-C6-HSL, C6-HSL, and C8-HSL (Figure 3). Strain T2-1-2 appeared to produce more 3-oxo-C6-HSL than T2-1-1.


N-acyl homoserine lactone production by Klebsiella pneumoniae isolated from human tongue surface.

Yin WF, Purmal K, Chin S, Chan XY, Koh CL, Sam CK, Chan KG - Sensors (Basel) (2012)

TLC analysis of AHL production by oral K. pneumoniae strains. Arrows indicate the positions of AHL standards run on the same plate. Lane A: C6-HSL (C6) and C8-HSL (C8). Lane B: 3-oxo-C6-HSL (OC6), C8-HSL (C8), and C10-HSL (C10). Lanes C and D: Extracts of spent culture supernatants from T2-1-1 and T2-1-2, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376583&req=5

f3-sensors-12-03472: TLC analysis of AHL production by oral K. pneumoniae strains. Arrows indicate the positions of AHL standards run on the same plate. Lane A: C6-HSL (C6) and C8-HSL (C8). Lane B: 3-oxo-C6-HSL (OC6), C8-HSL (C8), and C10-HSL (C10). Lanes C and D: Extracts of spent culture supernatants from T2-1-1 and T2-1-2, respectively.
Mentions: We succeeded in our initial objective of isolating quorum quenching bacteria from the human tongue surface (data not shown). We then tested these isolates for AHL production. To our surprise, isolates T2-1-1 and T2-1-2 produced AHLs, detected initially with A. tumefaciens NTL4(pZLR4) on agar plates–(data not shown). Subsequently, TLC results of the extracts of spent culture supernatants from T2-1-1 and T2-1-2 revealed three well-resolved spots with relative migration factor (Rf) values corresponding to those of 3-oxo-C6-HSL, C6-HSL, and C8-HSL (Figure 3). Strain T2-1-2 appeared to produce more 3-oxo-C6-HSL than T2-1-1.

Bottom Line: Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual.Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs.High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com

ABSTRACT
Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-L-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium.

Show MeSH
Related in: MedlinePlus