Limits...
Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge.

Frąc M, Oszust K, Lipiec J - Sensors (Basel) (2012)

Bottom Line: The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects.It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil.Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agrophysics, Polish Academy of Sciences, Lublin 27, Poland. m.frac@ipan.lublin.pl

ABSTRACT
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha(-1) and 26 Mg·ha(-1)) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

Show MeSH

Related in: MedlinePlus

Respiratory activity in soil 1 year after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical error bars represent the standard error of the mean.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3376570&req=5

f4-sensors-12-03253: Respiratory activity in soil 1 year after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical error bars represent the standard error of the mean.

Mentions: Low carbon availability is frequently a limiting factor for microbial growth [30,41,42]. It is worth noting that the use of the Biolog Eco-Plate™ assay with carbon substrates in our study showed changes in the microbial functional diversity after one year following the dairy sewage sludge application. These results are consistent with findings from an earlier experiment (Gomez et al. [24]), were significant increases in AWCD, R and H were measured several months after organic amendment application. Moreover, we noticed that the above mentioned CLPP indices were highly positively correlated to each other (r = 0.74–0.99) (Table 5). Comparison of Figures 1, 3 and 4 indicates that treatment effects on microbial activity were reflected similarly by CLPP indices, dehydrogenase activity and respiratory activity. Dehydrogenase activity under DSSLD with large dose of the sewage sludge was significantly greater (p < 0.05) compared to other treatments. Positive effect of dairy sludge application on dehydrogenase activity was also observed in our earlier field study [43]. As can be seen from Table 5 the dehydrogenase activity was significantly correlated with AWCD (p < 0.01).


Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge.

Frąc M, Oszust K, Lipiec J - Sensors (Basel) (2012)

Respiratory activity in soil 1 year after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical error bars represent the standard error of the mean.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376570&req=5

f4-sensors-12-03253: Respiratory activity in soil 1 year after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical error bars represent the standard error of the mean.
Mentions: Low carbon availability is frequently a limiting factor for microbial growth [30,41,42]. It is worth noting that the use of the Biolog Eco-Plate™ assay with carbon substrates in our study showed changes in the microbial functional diversity after one year following the dairy sewage sludge application. These results are consistent with findings from an earlier experiment (Gomez et al. [24]), were significant increases in AWCD, R and H were measured several months after organic amendment application. Moreover, we noticed that the above mentioned CLPP indices were highly positively correlated to each other (r = 0.74–0.99) (Table 5). Comparison of Figures 1, 3 and 4 indicates that treatment effects on microbial activity were reflected similarly by CLPP indices, dehydrogenase activity and respiratory activity. Dehydrogenase activity under DSSLD with large dose of the sewage sludge was significantly greater (p < 0.05) compared to other treatments. Positive effect of dairy sludge application on dehydrogenase activity was also observed in our earlier field study [43]. As can be seen from Table 5 the dehydrogenase activity was significantly correlated with AWCD (p < 0.01).

Bottom Line: The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects.It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil.Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agrophysics, Polish Academy of Sciences, Lublin 27, Poland. m.frac@ipan.lublin.pl

ABSTRACT
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha(-1) and 26 Mg·ha(-1)) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

Show MeSH
Related in: MedlinePlus