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Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge.

Frąc M, Oszust K, Lipiec J - Sensors (Basel) (2012)

Bottom Line: The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects.It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil.Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agrophysics, Polish Academy of Sciences, Lublin 27, Poland. m.frac@ipan.lublin.pl

ABSTRACT
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha(-1) and 26 Mg·ha(-1)) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

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Related in: MedlinePlus

Percent of total carbon source utilization response tracked due to treatment type, for the different guilds–amines and amides (A&A), amino acids (AA), carboxylic and acetic acids (C&AA), polymers (Poly) and carbohydrates (Carb). Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose.
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f2-sensors-12-03253: Percent of total carbon source utilization response tracked due to treatment type, for the different guilds–amines and amides (A&A), amino acids (AA), carboxylic and acetic acids (C&AA), polymers (Poly) and carbohydrates (Carb). Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose.

Mentions: The substrates situated on microtiter plates were divided into five main groups: carbohydrates, carboxylic and acetic acids, amino acids, polymers, amines and amides. Their relative contributions in soil amended with dairy sewage sludge and mineral fertilization were examined. Figure 2 presents the percentage of total carbon source utilization for each guild (see Table 3 for class groupings) after amendment application.


Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge.

Frąc M, Oszust K, Lipiec J - Sensors (Basel) (2012)

Percent of total carbon source utilization response tracked due to treatment type, for the different guilds–amines and amides (A&A), amino acids (AA), carboxylic and acetic acids (C&AA), polymers (Poly) and carbohydrates (Carb). Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376570&req=5

f2-sensors-12-03253: Percent of total carbon source utilization response tracked due to treatment type, for the different guilds–amines and amides (A&A), amino acids (AA), carboxylic and acetic acids (C&AA), polymers (Poly) and carbohydrates (Carb). Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose.
Mentions: The substrates situated on microtiter plates were divided into five main groups: carbohydrates, carboxylic and acetic acids, amino acids, polymers, amines and amides. Their relative contributions in soil amended with dairy sewage sludge and mineral fertilization were examined. Figure 2 presents the percentage of total carbon source utilization for each guild (see Table 3 for class groupings) after amendment application.

Bottom Line: The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects.It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil.Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agrophysics, Polish Academy of Sciences, Lublin 27, Poland. m.frac@ipan.lublin.pl

ABSTRACT
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha(-1) and 26 Mg·ha(-1)) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

Show MeSH
Related in: MedlinePlus