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Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge.

Frąc M, Oszust K, Lipiec J - Sensors (Basel) (2012)

Bottom Line: The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects.It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil.Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agrophysics, Polish Academy of Sciences, Lublin 27, Poland. m.frac@ipan.lublin.pl

ABSTRACT
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha(-1) and 26 Mg·ha(-1)) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

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Related in: MedlinePlus

(a) Average well-color development (AWCD); (b) Richness (R) and (c) Shannon–Weaver index (H) of metabolized substrates in Biolog EcoPlate™ after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical bars represent the standard error of the mean. Treatment means separated by different letters are significantly different (Tukey’s mean separation test, P < 0.05).
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f1-sensors-12-03253: (a) Average well-color development (AWCD); (b) Richness (R) and (c) Shannon–Weaver index (H) of metabolized substrates in Biolog EcoPlate™ after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical bars represent the standard error of the mean. Treatment means separated by different letters are significantly different (Tukey’s mean separation test, P < 0.05).

Mentions: As demonstrated by Figure 1, the AWCD, R, H indexes from the dairy sewage sludge amended soil were significantly (P < 0.05) greater than in the mineral fertilized and control soil. This indicates a greater rate of substrate utilization (catabolic potential) by the microbial community and greater functional diversity in the sewage-amended than in mineral fertilized soils. However, there were no significant differences between the indices of dairy sewage sludge (DSS) amended soil and soil under long-term undisturbed native vegetation. The results indicated higher values of AWCD, H and R indices in undisturbed native soil compared to the control. These differences were probably due to the presence of more complex organic material such as plant and root debris in undisturbed site and also root exudates of a greater variety of plants species growing on undisturbed native than control soil, on which only monoculture of winter wheat was cropped.


Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge.

Frąc M, Oszust K, Lipiec J - Sensors (Basel) (2012)

(a) Average well-color development (AWCD); (b) Richness (R) and (c) Shannon–Weaver index (H) of metabolized substrates in Biolog EcoPlate™ after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical bars represent the standard error of the mean. Treatment means separated by different letters are significantly different (Tukey’s mean separation test, P < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376570&req=5

f1-sensors-12-03253: (a) Average well-color development (AWCD); (b) Richness (R) and (c) Shannon–Weaver index (H) of metabolized substrates in Biolog EcoPlate™ after amendment application. Explanations: UND–undisturbed native vegetation, C–control soil without mineral and organic fertilization, MFSD–mineral fertilization amendment, small dose, MFLD–mineral fertilization amendment, large dose, DSSSD–dairy sewage sludge, small dose, DSSLD–dairy sewage sludge, large dose, n = 3. Vertical bars represent the standard error of the mean. Treatment means separated by different letters are significantly different (Tukey’s mean separation test, P < 0.05).
Mentions: As demonstrated by Figure 1, the AWCD, R, H indexes from the dairy sewage sludge amended soil were significantly (P < 0.05) greater than in the mineral fertilized and control soil. This indicates a greater rate of substrate utilization (catabolic potential) by the microbial community and greater functional diversity in the sewage-amended than in mineral fertilized soils. However, there were no significant differences between the indices of dairy sewage sludge (DSS) amended soil and soil under long-term undisturbed native vegetation. The results indicated higher values of AWCD, H and R indices in undisturbed native soil compared to the control. These differences were probably due to the presence of more complex organic material such as plant and root debris in undisturbed site and also root exudates of a greater variety of plants species growing on undisturbed native than control soil, on which only monoculture of winter wheat was cropped.

Bottom Line: The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects.It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil.Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

View Article: PubMed Central - PubMed

Affiliation: Institute of Agrophysics, Polish Academy of Sciences, Lublin 27, Poland. m.frac@ipan.lublin.pl

ABSTRACT
The aim of the present work was to assess the influence of organic amendment applications compared to mineral fertilization on soil microbial activity and functional diversity. The field experiment was set up on a soil classified as an Eutric Cambisol developed from loess (South-East Poland). Two doses of both dairy sewage sludge (20 Mg·ha(-1) and 26 Mg·ha(-1)) and of mineral fertilizers containing the same amount of nutrients were applied. The same soil without any amendment was used as a control. The soil under undisturbed native vegetation was also included in the study as a representative background sample. The functional diversity (catabolic potential) was assessed using such indices as Average Well Color Development (AWCD), Richness (R) and Shannon-Weaver index (H). These indices were calculated, following the community level physiological profiling (CLPP) using Biolog Eco Plates. Soil dehydrogenase and respiratory activity were also evaluated. The indices were sensitive enough to reveal changes in community level physiological profiles due to treatment effects. It was shown that dairy sewage amended soil was characterized by greater AWCD, R, H and dehydrogenase and respiratory activity as compared to control or mineral fertilized soil. Analysis of variance (ANOVA) and principal component analysis (PCA) were used to depict the differences of the soil bacterial functional diversity between the treatments.

Show MeSH
Related in: MedlinePlus