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Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

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Related in: MedlinePlus

Fabrication of Pd electrode.
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f7-sensors-12-02400: Fabrication of Pd electrode.

Mentions: We used Pd electrodes to construct an EOF-driven microelectrode. A layer of chromium film should first be evaporated onto a glass substrate before the evaporation of the Pd film. The spacing between the triangle tip electrodes was maintained at 100 μm (width), as shown in the mask map in Figure 7. The power amplifier generated a sine wave signal (AC) with a voltage of 15 V and a frequency of 1 MHz. The electric field generated by the AC in the tiny space induced the inner and outer cell membrane into generating opposing electrical distributions, which forms a squeeze pressure on the inner and outer cell membranes because of electrical attraction. This causes damage given the thinning of the cell membrane, resulting in irreversible electrical breakdown from cell membrane rupture. Suitable voltage control can be used to control the damage outside or inside the cell. The principle of cell lysis by voltage lies in compressing the cell membrane structure primarily with the mechanism of the electric field. When the electric field is large enough, the opposite electricity is imposed upon the interior and exterior membranes of the cell. Then, the interior and exterior membranes become thinner because of the compressed pressure. After this, the cell membrane is destroyed [5].


Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Fabrication of Pd electrode.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376563&req=5

f7-sensors-12-02400: Fabrication of Pd electrode.
Mentions: We used Pd electrodes to construct an EOF-driven microelectrode. A layer of chromium film should first be evaporated onto a glass substrate before the evaporation of the Pd film. The spacing between the triangle tip electrodes was maintained at 100 μm (width), as shown in the mask map in Figure 7. The power amplifier generated a sine wave signal (AC) with a voltage of 15 V and a frequency of 1 MHz. The electric field generated by the AC in the tiny space induced the inner and outer cell membrane into generating opposing electrical distributions, which forms a squeeze pressure on the inner and outer cell membranes because of electrical attraction. This causes damage given the thinning of the cell membrane, resulting in irreversible electrical breakdown from cell membrane rupture. Suitable voltage control can be used to control the damage outside or inside the cell. The principle of cell lysis by voltage lies in compressing the cell membrane structure primarily with the mechanism of the electric field. When the electric field is large enough, the opposite electricity is imposed upon the interior and exterior membranes of the cell. Then, the interior and exterior membranes become thinner because of the compressed pressure. After this, the cell membrane is destroyed [5].

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

Show MeSH
Related in: MedlinePlus