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Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

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Related in: MedlinePlus

The fuzzy output rule in this control platform.
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f6-sensors-12-02400: The fuzzy output rule in this control platform.

Mentions: The voltage controller is developed basing on the center of gravity defuzzification. Given that the size of the acquired image frame is 640 × 480 pixels in length and width, its diagonal length is 800 pixels. The largest movement distance is 800 pixels when the tracked cell and target location are located at the end point of the diagonal, and the smallest movement distance is the radius of the tracked cell. The movement distance changes with cell size and radius ratio setting. The fuzzy membership functions are shown in Equation (2):(2a)μ(small)(x)=−(x−240)240−(Radius x radio),(Radius x radio)≤x≤240(2b)μ(medium)(x)=(x−Radius)240−Radius,Radius≤x≤240(2c)μ(medium)(x)=−(x−800)800−240,240≤x≤800(2d)μ(large)(x)=(x−240)800−240,240≤x≤800where Radius is the radius of the tracked cell, and ratio is a scale parameter. We define, if the Error (Distance) value is less than the Cell Radius x ratio, it is successful for positioning. The value μ(x) is called the membership degree of x in the fuzzy set. The membership degree μ(x) quantifies the grade of membership of the element x to the fuzzy set. The value 0 indicates that x is not a member of the fuzzy set; the value 1 means that x is fully a member of the fuzzy set. The movement distance data were processed and transfer to output control voltage by the triangle center of gravity defuzzification [10,11]. The fuzzy output rule is shown in Figure 6. For example, if the voltage range is set as 9.2∼19.76 V (pixel range is 170∼215), when the movement distance between the tracked cell and the target location is 616 pixels (long distance), the radius is 20 and the ratio is 1 as shown in Figure 6(a). The value of μ(medium)(x) is 0.3286 and μ(large)(x) is 0.6714. The digital output (Do) is 201 according as the triangle center of gravity defuzzification. The output voltage (Vo) is relayed to the Do, as is shown in Equation (3):(3)V0=0.2357 Do−30.897where Do = distance (pixels), Vo = output voltage (voltage). The real Vo is 16.48 volts, as shown in Figure 6(b).


Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

The fuzzy output rule in this control platform.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376563&req=5

f6-sensors-12-02400: The fuzzy output rule in this control platform.
Mentions: The voltage controller is developed basing on the center of gravity defuzzification. Given that the size of the acquired image frame is 640 × 480 pixels in length and width, its diagonal length is 800 pixels. The largest movement distance is 800 pixels when the tracked cell and target location are located at the end point of the diagonal, and the smallest movement distance is the radius of the tracked cell. The movement distance changes with cell size and radius ratio setting. The fuzzy membership functions are shown in Equation (2):(2a)μ(small)(x)=−(x−240)240−(Radius x radio),(Radius x radio)≤x≤240(2b)μ(medium)(x)=(x−Radius)240−Radius,Radius≤x≤240(2c)μ(medium)(x)=−(x−800)800−240,240≤x≤800(2d)μ(large)(x)=(x−240)800−240,240≤x≤800where Radius is the radius of the tracked cell, and ratio is a scale parameter. We define, if the Error (Distance) value is less than the Cell Radius x ratio, it is successful for positioning. The value μ(x) is called the membership degree of x in the fuzzy set. The membership degree μ(x) quantifies the grade of membership of the element x to the fuzzy set. The value 0 indicates that x is not a member of the fuzzy set; the value 1 means that x is fully a member of the fuzzy set. The movement distance data were processed and transfer to output control voltage by the triangle center of gravity defuzzification [10,11]. The fuzzy output rule is shown in Figure 6. For example, if the voltage range is set as 9.2∼19.76 V (pixel range is 170∼215), when the movement distance between the tracked cell and the target location is 616 pixels (long distance), the radius is 20 and the ratio is 1 as shown in Figure 6(a). The value of μ(medium)(x) is 0.3286 and μ(large)(x) is 0.6714. The digital output (Do) is 201 according as the triangle center of gravity defuzzification. The output voltage (Vo) is relayed to the Do, as is shown in Equation (3):(3)V0=0.2357 Do−30.897where Do = distance (pixels), Vo = output voltage (voltage). The real Vo is 16.48 volts, as shown in Figure 6(b).

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

Show MeSH
Related in: MedlinePlus