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Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

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Related in: MedlinePlus

Filament DNA image.
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f11-sensors-12-02400: Filament DNA image.

Mentions: After cell lysis, the fluorescent dye YO-PRO-1 was added to the lysis absorbing cell solution, which was then observed under a microscope. Through a 100× immersion lens and B-cell nucleus radiation, the DNA image of the post-lysis U937 cell could be observed in Figure 11. This proves that the trans-membrane formed by the combination of the tip microelectrode and AC in this system architecture can succeed in cell lysis for intracellular DNA extraction.


Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Filament DNA image.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376563&req=5

f11-sensors-12-02400: Filament DNA image.
Mentions: After cell lysis, the fluorescent dye YO-PRO-1 was added to the lysis absorbing cell solution, which was then observed under a microscope. Through a 100× immersion lens and B-cell nucleus radiation, the DNA image of the post-lysis U937 cell could be observed in Figure 11. This proves that the trans-membrane formed by the combination of the tip microelectrode and AC in this system architecture can succeed in cell lysis for intracellular DNA extraction.

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

Show MeSH
Related in: MedlinePlus