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Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

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Related in: MedlinePlus

Diagram of the system configuration.
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f1-sensors-12-02400: Diagram of the system configuration.

Mentions: The experiment was conducted on an inverse microscope. The electric platform, microscope CCD, and image acquirement card were used to capture real-time images. The local area network (LAN) control signal generator was used to project sine wave signals to lyse cells. The configuration of the entire system is shown in Figure 1. The man-machine interface package software in LabVIEW was used to create the machine vision control system to track a single cell and move it to a specific position and lyse the cells via electroporation. The man-machine interface panel is shown in Figure 2. The human-machine interface for the image processing of cell tracking is also shown in Figure 2, which illustrates an image as interpreted by the image processer as an image of a cell. Cells seen on the screen that have a yellow circle around their outer ring represent cells that have been interpreted successfully. If no yellow circle appears around the outer ring of a cell, then that cell has not been interpreted successfully by the system. First, the selected cells were tracked. The cells to be tracked were chosen from the capture screen using the mouse to mark the target by pointing and clicking on the selected cells. The tracking system locates the target cells to be tracked so as to complete the tracking behavior. The center of the target cell being tracked shows a cross symbol, and the tracking light in the LabVIEW program turns on to indicate that tracking is successful. The system automatically uses the target coordinates as the center, and uses the set value of the “Ratio” function on the control panel multiplied by the radius of the tracking cell as the localization radius, to draw a circle as the target criterion.


Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Diagram of the system configuration.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376563&req=5

f1-sensors-12-02400: Diagram of the system configuration.
Mentions: The experiment was conducted on an inverse microscope. The electric platform, microscope CCD, and image acquirement card were used to capture real-time images. The local area network (LAN) control signal generator was used to project sine wave signals to lyse cells. The configuration of the entire system is shown in Figure 1. The man-machine interface package software in LabVIEW was used to create the machine vision control system to track a single cell and move it to a specific position and lyse the cells via electroporation. The man-machine interface panel is shown in Figure 2. The human-machine interface for the image processing of cell tracking is also shown in Figure 2, which illustrates an image as interpreted by the image processer as an image of a cell. Cells seen on the screen that have a yellow circle around their outer ring represent cells that have been interpreted successfully. If no yellow circle appears around the outer ring of a cell, then that cell has not been interpreted successfully by the system. First, the selected cells were tracked. The cells to be tracked were chosen from the capture screen using the mouse to mark the target by pointing and clicking on the selected cells. The tracking system locates the target cells to be tracked so as to complete the tracking behavior. The center of the target cell being tracked shows a cross symbol, and the tracking light in the LabVIEW program turns on to indicate that tracking is successful. The system automatically uses the target coordinates as the center, and uses the set value of the “Ratio” function on the control panel multiplied by the radius of the tracking cell as the localization radius, to draw a circle as the target criterion.

Bottom Line: The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

Show MeSH
Related in: MedlinePlus