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New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.

Wang Y, Ye Z, Ying Y - Sensors (Basel) (2012)

Bottom Line: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element.In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wang_yi_xian1986@sina.com

ABSTRACT
The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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(a) IDAM chip with gold microelectrodes on a glass wafer, (b) a microchannel with a detection microchamber, and inlet and outlet channels, and (c) an assembled microfluidic flow cell with embedded IDAM and connection wires [84].
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f5-sensors-12-03449: (a) IDAM chip with gold microelectrodes on a glass wafer, (b) a microchannel with a detection microchamber, and inlet and outlet channels, and (c) an assembled microfluidic flow cell with embedded IDAM and connection wires [84].

Mentions: Besides nanomaterials, microfluidics techniques are a good strategy for improving the performance of impedimetric bacteria biosensors [86–94]. Microfluidics techniques in general seek to improve analytical performance by reducing the consumption of reagents, decreasing the analysis time, increasing reliability and sensitivity through automation, and integrating multiple processes in a single device. These features are particularly suitable for hand-held impedance biosensors for bacteria detection [95]. Varshney et al. [84] integrated a microfluidics flow cell with embedded gold IDAM into an impedance biosensor to rapidly detect pathogenic bacteria in ground beef samples. The flow cell consisting of a detection microchamber and inlet and outlet microchannels was fabricated by binding an IDAM chip to a poly (dimethylsiloxane) (PDMS) microchannel (Figure 5). The detection microchamber with a dimension of 6 mm × 0.5 mm × 0.02 mm and a volume of 60 nL was used to collect bacterial cells in the active layer above the microelectrode for sensitive impedance change. Antibody coated magnetic nanoparticles were used to specifically separate and concentrate the target bacteria and then the biomolecule functional magnetic nanoparticles-bacteria complexes were injected into microfluidic cell to detect the impedance change. Using the microfluidic system, the limit of detection has been improved an order of magnitude as low as 1.6 × 102 and 1.2 × 103 cfu·mL−1 of E. coli O157:H7 cells present in pure culture and ground beef sample, respectively. Tan et al. [82] devised a PDMS microfluidic immunosensor integrated with specific antibody immobilized alumina nanoporous membrane for rapid detection of foodborne pathogens E. coli O157:H7 and Staphylococcus aureus with EIS. When the target bacteria were injected into the chamber to bind antibody, the electrolyte current will be blocked which can be monitored by the impedance spectrum. This microfluidic immunosensor based on nanoporous membrane impedance spectrum could achieve rapid bacteria detection within 2 h with a high sensitivity of 102 cfu mL−1.


New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.

Wang Y, Ye Z, Ying Y - Sensors (Basel) (2012)

(a) IDAM chip with gold microelectrodes on a glass wafer, (b) a microchannel with a detection microchamber, and inlet and outlet channels, and (c) an assembled microfluidic flow cell with embedded IDAM and connection wires [84].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376556&req=5

f5-sensors-12-03449: (a) IDAM chip with gold microelectrodes on a glass wafer, (b) a microchannel with a detection microchamber, and inlet and outlet channels, and (c) an assembled microfluidic flow cell with embedded IDAM and connection wires [84].
Mentions: Besides nanomaterials, microfluidics techniques are a good strategy for improving the performance of impedimetric bacteria biosensors [86–94]. Microfluidics techniques in general seek to improve analytical performance by reducing the consumption of reagents, decreasing the analysis time, increasing reliability and sensitivity through automation, and integrating multiple processes in a single device. These features are particularly suitable for hand-held impedance biosensors for bacteria detection [95]. Varshney et al. [84] integrated a microfluidics flow cell with embedded gold IDAM into an impedance biosensor to rapidly detect pathogenic bacteria in ground beef samples. The flow cell consisting of a detection microchamber and inlet and outlet microchannels was fabricated by binding an IDAM chip to a poly (dimethylsiloxane) (PDMS) microchannel (Figure 5). The detection microchamber with a dimension of 6 mm × 0.5 mm × 0.02 mm and a volume of 60 nL was used to collect bacterial cells in the active layer above the microelectrode for sensitive impedance change. Antibody coated magnetic nanoparticles were used to specifically separate and concentrate the target bacteria and then the biomolecule functional magnetic nanoparticles-bacteria complexes were injected into microfluidic cell to detect the impedance change. Using the microfluidic system, the limit of detection has been improved an order of magnitude as low as 1.6 × 102 and 1.2 × 103 cfu·mL−1 of E. coli O157:H7 cells present in pure culture and ground beef sample, respectively. Tan et al. [82] devised a PDMS microfluidic immunosensor integrated with specific antibody immobilized alumina nanoporous membrane for rapid detection of foodborne pathogens E. coli O157:H7 and Staphylococcus aureus with EIS. When the target bacteria were injected into the chamber to bind antibody, the electrolyte current will be blocked which can be monitored by the impedance spectrum. This microfluidic immunosensor based on nanoporous membrane impedance spectrum could achieve rapid bacteria detection within 2 h with a high sensitivity of 102 cfu mL−1.

Bottom Line: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element.In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wang_yi_xian1986@sina.com

ABSTRACT
The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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