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New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.

Wang Y, Ye Z, Ying Y - Sensors (Basel) (2012)

Bottom Line: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element.In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wang_yi_xian1986@sina.com

ABSTRACT
The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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(A) Principle of TiO2 nanowire bundle microelectrode based impedance immunosensor for the detection of bacteria. (B) SEM micrographs of TiO2 nanowire bundle (a) before (5,000×) and (b,c) after binding with Listeria innocua (20,000×) [72].
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f4-sensors-12-03449: (A) Principle of TiO2 nanowire bundle microelectrode based impedance immunosensor for the detection of bacteria. (B) SEM micrographs of TiO2 nanowire bundle (a) before (5,000×) and (b,c) after binding with Listeria innocua (20,000×) [72].

Mentions: In addition to nanoparticles, nanowires have been attracted much scientific interest in analytical chemistry, especially in biosensing technologies. This is due to their unique semiconductive properties associated with the nanostructures, and they are believed to be ultrasensitive in performing single molecule sensing. Wang et al. [72] developed a TiO2 nanowire bundle microelectrode based impedimetric immunosensor for rapid and sensitive detection of Listeria monocytogenes. TiO2 nanowire bundle was connected to gold microelectrodes using mask welding and then monoclonal antibodies were immobilized on the surface of a TiO2 nanowire bundle to specifically capture bacteria (Figure 4). Impedance changes caused by the nanowire-antibody-bacteria complex were measured and correlated to bacterial number. Since the TiO2 nanowires can be highly oriented on substrates or form free-standing membranes, the fabricated electrode showed a large specific surface area, good biocompatibility, good chemical and photochemical stabilities, and negligible protein denaturation. This nanowire bundle based immunosensor also exhibited a good performance that can detect as low as 102 cfu·mL−1 of Listeria monocytogenes in 1 h without significant interference from other foodborne pathogens.


New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.

Wang Y, Ye Z, Ying Y - Sensors (Basel) (2012)

(A) Principle of TiO2 nanowire bundle microelectrode based impedance immunosensor for the detection of bacteria. (B) SEM micrographs of TiO2 nanowire bundle (a) before (5,000×) and (b,c) after binding with Listeria innocua (20,000×) [72].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376556&req=5

f4-sensors-12-03449: (A) Principle of TiO2 nanowire bundle microelectrode based impedance immunosensor for the detection of bacteria. (B) SEM micrographs of TiO2 nanowire bundle (a) before (5,000×) and (b,c) after binding with Listeria innocua (20,000×) [72].
Mentions: In addition to nanoparticles, nanowires have been attracted much scientific interest in analytical chemistry, especially in biosensing technologies. This is due to their unique semiconductive properties associated with the nanostructures, and they are believed to be ultrasensitive in performing single molecule sensing. Wang et al. [72] developed a TiO2 nanowire bundle microelectrode based impedimetric immunosensor for rapid and sensitive detection of Listeria monocytogenes. TiO2 nanowire bundle was connected to gold microelectrodes using mask welding and then monoclonal antibodies were immobilized on the surface of a TiO2 nanowire bundle to specifically capture bacteria (Figure 4). Impedance changes caused by the nanowire-antibody-bacteria complex were measured and correlated to bacterial number. Since the TiO2 nanowires can be highly oriented on substrates or form free-standing membranes, the fabricated electrode showed a large specific surface area, good biocompatibility, good chemical and photochemical stabilities, and negligible protein denaturation. This nanowire bundle based immunosensor also exhibited a good performance that can detect as low as 102 cfu·mL−1 of Listeria monocytogenes in 1 h without significant interference from other foodborne pathogens.

Bottom Line: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element.In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wang_yi_xian1986@sina.com

ABSTRACT
The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

Show MeSH