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New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.

Wang Y, Ye Z, Ying Y - Sensors (Basel) (2012)

Bottom Line: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element.In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wang_yi_xian1986@sina.com

ABSTRACT
The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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Principle of impedance coupled with dielectrophoresis and electropermeabilization.
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f3-sensors-12-03449: Principle of impedance coupled with dielectrophoresis and electropermeabilization.

Mentions: Recently, some researchers have combined the dielectrophoretic impedance measurement (DEPIM) technique with impedimetric biosensors for bacteria detection (Figure 3) [35,36]. The DEPIM utilizes the positive dielectrophoretic force to trap suspended biological cells onto the electrode in the form of pearl chains and then measured an impedance signal [37–39], which can detect bacteria in shorter time than traditional impedance methods due to the effect of dielectrophoretic force. Suehiro et al. [40] developed a selective detection method for specific bacteria by using a DEPIM method in conjunction with an antigen-antibody reaction. Antibodies were immobilized on the electrode chip before the preliminary bacteria trapping by positive dielectrophoresis (DEP). The bacteria were attracted to the electrode gap under the action of the positive DEP force and finally brought into contact with the glass surface to be bound with the immobilized antibodies. It was also confirmed that the proposed method realized selective detection of the target bacteria from a mixed suspension with non-target bacteria.


New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.

Wang Y, Ye Z, Ying Y - Sensors (Basel) (2012)

Principle of impedance coupled with dielectrophoresis and electropermeabilization.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376556&req=5

f3-sensors-12-03449: Principle of impedance coupled with dielectrophoresis and electropermeabilization.
Mentions: Recently, some researchers have combined the dielectrophoretic impedance measurement (DEPIM) technique with impedimetric biosensors for bacteria detection (Figure 3) [35,36]. The DEPIM utilizes the positive dielectrophoretic force to trap suspended biological cells onto the electrode in the form of pearl chains and then measured an impedance signal [37–39], which can detect bacteria in shorter time than traditional impedance methods due to the effect of dielectrophoretic force. Suehiro et al. [40] developed a selective detection method for specific bacteria by using a DEPIM method in conjunction with an antigen-antibody reaction. Antibodies were immobilized on the electrode chip before the preliminary bacteria trapping by positive dielectrophoresis (DEP). The bacteria were attracted to the electrode gap under the action of the positive DEP force and finally brought into contact with the glass surface to be bound with the immobilized antibodies. It was also confirmed that the proposed method realized selective detection of the target bacteria from a mixed suspension with non-target bacteria.

Bottom Line: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security.The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element.In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. wang_yi_xian1986@sina.com

ABSTRACT
The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.

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