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JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus

Effect of JCM-16021 on mucosal cytokines production in PI-IBS rats. Statistical graphs about mucosal cytokines production are shown in (a). The rates of cytokines alteration are shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus normal rats, #P < 0.05 versus PI-IBS rats (t-test).
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fig6: Effect of JCM-16021 on mucosal cytokines production in PI-IBS rats. Statistical graphs about mucosal cytokines production are shown in (a). The rates of cytokines alteration are shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus normal rats, #P < 0.05 versus PI-IBS rats (t-test).

Mentions: As shown in Figure 6(a), the levels of TNF-α, IFN-γ, IL-6, and IL-10 in the colonic mucosa of PI-IBS rats were all significantly decreased when compared to that of the normal rats (P < 0.05). High dose of JCM-16021 treatment significantly elevated the levels of TNF-α, IL-6, and IL-10, but not IFN-γ, in PI-IBS rats, suggesting that JCM-16021 may modulate mucosal cytokines production in PI-IBS rats. In pCPA-treated rats, the cytokine levels were not changed when compared to that of the PI-IBS rats, indicating that 5-HT depletion may have little effect on cytokines production of PI-IBS rats. As shown in Figure 6(b), even though the detected cytokines were all decreased significantly in PI-IBS rats, the levels of Th1-related cytokines, TNF-α and IFN-γ, were decreased more obviously (51% decrease in TNF-α, 42% decrease in IFN-γ) than others (14% decrease in IL-10, 19% decrease in IL-6).


JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Effect of JCM-16021 on mucosal cytokines production in PI-IBS rats. Statistical graphs about mucosal cytokines production are shown in (a). The rates of cytokines alteration are shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus normal rats, #P < 0.05 versus PI-IBS rats (t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376539&req=5

fig6: Effect of JCM-16021 on mucosal cytokines production in PI-IBS rats. Statistical graphs about mucosal cytokines production are shown in (a). The rates of cytokines alteration are shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus normal rats, #P < 0.05 versus PI-IBS rats (t-test).
Mentions: As shown in Figure 6(a), the levels of TNF-α, IFN-γ, IL-6, and IL-10 in the colonic mucosa of PI-IBS rats were all significantly decreased when compared to that of the normal rats (P < 0.05). High dose of JCM-16021 treatment significantly elevated the levels of TNF-α, IL-6, and IL-10, but not IFN-γ, in PI-IBS rats, suggesting that JCM-16021 may modulate mucosal cytokines production in PI-IBS rats. In pCPA-treated rats, the cytokine levels were not changed when compared to that of the PI-IBS rats, indicating that 5-HT depletion may have little effect on cytokines production of PI-IBS rats. As shown in Figure 6(b), even though the detected cytokines were all decreased significantly in PI-IBS rats, the levels of Th1-related cytokines, TNF-α and IFN-γ, were decreased more obviously (51% decrease in TNF-α, 42% decrease in IFN-γ) than others (14% decrease in IL-10, 19% decrease in IL-6).

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus