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JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus

Effect of JCM-16021 on mechanical stimuli (CRD) induced 5-HT release in PI-IBS rats. (a) shows the statistical analysis of colonic 5-HT content in PI-IBS rats with or without CRD treatment. (b) shows the representative electron microsgraphs of EC cells and their secretory granules (inset) from the (B1) normal rats, (B2) PI-IBS rats, and (B3) high dose JCM-16021 treated rats (×8900; inset: ×10000). Activated EC cell is characterized by the clear secretory granules without cores (red arrow), or secretory granules with eccentric cores (red arrowhead) in it. Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus the same group without CRD treatment (t-test).
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fig5: Effect of JCM-16021 on mechanical stimuli (CRD) induced 5-HT release in PI-IBS rats. (a) shows the statistical analysis of colonic 5-HT content in PI-IBS rats with or without CRD treatment. (b) shows the representative electron microsgraphs of EC cells and their secretory granules (inset) from the (B1) normal rats, (B2) PI-IBS rats, and (B3) high dose JCM-16021 treated rats (×8900; inset: ×10000). Activated EC cell is characterized by the clear secretory granules without cores (red arrow), or secretory granules with eccentric cores (red arrowhead) in it. Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus the same group without CRD treatment (t-test).

Mentions: To identify whether mechanical stimuli (CRD) can induce excessive 5-HT release in PI-IBS rats and the effect of JCM-16021 on it, colonic 5-HT content was evaluated in rats with or without AWR test. As shown in Figure 5(a), after CRD application, the 5-HT content of normal rats reduced by ~17% when compared to that without CRD, but no statistical differences were found between these two groups; while in PI-IBS rats, 5-HT content dramatically decreased by ~68% after CRD application (P < 0.01). In pCPA-treated rats, CRD also induced marked decrease of 5-HT content in PI-IBS rats (~48%, P < 0.05), even though the baseline 5-HT content has been lowered below the normal level. After high and medium dose of JCM-16021 treatment, CRD did not induce significant decreases of 5-HT content (~35% and ~12%) when compared to that without CRD application, suggesting that the treatment of high and medium dose of JCM-16021 may attenuate CRD-induced excessive 5-HT release in PI-IBS rats. Results from electronic microsgraphs also showed that the secretory granules within EC cells of PI-IBS rats were characterized by clear secretory granules without cores or empty vesicles after CRD application, indicating that there may be an excessive 5-HT release in PI-IBS rats after mechanical stimulation. In high-dose JCM-16021-treated group, the secretory granules within EC cells showed little empty vesicles when compared to that of the PI-IBS rats (Figure 5(b)).


JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Effect of JCM-16021 on mechanical stimuli (CRD) induced 5-HT release in PI-IBS rats. (a) shows the statistical analysis of colonic 5-HT content in PI-IBS rats with or without CRD treatment. (b) shows the representative electron microsgraphs of EC cells and their secretory granules (inset) from the (B1) normal rats, (B2) PI-IBS rats, and (B3) high dose JCM-16021 treated rats (×8900; inset: ×10000). Activated EC cell is characterized by the clear secretory granules without cores (red arrow), or secretory granules with eccentric cores (red arrowhead) in it. Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus the same group without CRD treatment (t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3376539&req=5

fig5: Effect of JCM-16021 on mechanical stimuli (CRD) induced 5-HT release in PI-IBS rats. (a) shows the statistical analysis of colonic 5-HT content in PI-IBS rats with or without CRD treatment. (b) shows the representative electron microsgraphs of EC cells and their secretory granules (inset) from the (B1) normal rats, (B2) PI-IBS rats, and (B3) high dose JCM-16021 treated rats (×8900; inset: ×10000). Activated EC cell is characterized by the clear secretory granules without cores (red arrow), or secretory granules with eccentric cores (red arrowhead) in it. Data are shown as mean ± S.E.M., n = 5 per group. *P < 0.05, **P < 0.01 versus the same group without CRD treatment (t-test).
Mentions: To identify whether mechanical stimuli (CRD) can induce excessive 5-HT release in PI-IBS rats and the effect of JCM-16021 on it, colonic 5-HT content was evaluated in rats with or without AWR test. As shown in Figure 5(a), after CRD application, the 5-HT content of normal rats reduced by ~17% when compared to that without CRD, but no statistical differences were found between these two groups; while in PI-IBS rats, 5-HT content dramatically decreased by ~68% after CRD application (P < 0.01). In pCPA-treated rats, CRD also induced marked decrease of 5-HT content in PI-IBS rats (~48%, P < 0.05), even though the baseline 5-HT content has been lowered below the normal level. After high and medium dose of JCM-16021 treatment, CRD did not induce significant decreases of 5-HT content (~35% and ~12%) when compared to that without CRD application, suggesting that the treatment of high and medium dose of JCM-16021 may attenuate CRD-induced excessive 5-HT release in PI-IBS rats. Results from electronic microsgraphs also showed that the secretory granules within EC cells of PI-IBS rats were characterized by clear secretory granules without cores or empty vesicles after CRD application, indicating that there may be an excessive 5-HT release in PI-IBS rats after mechanical stimulation. In high-dose JCM-16021-treated group, the secretory granules within EC cells showed little empty vesicles when compared to that of the PI-IBS rats (Figure 5(b)).

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus