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JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus

Effect of JCM-16021 on mucosal SERT expression in PI-IBS rats. (a) shows the immunofluorescence micrographs of (A1) negative control (primary antibody omitted) and the positive SERT expressions (arrowhead) in the mucosa of (A2) normal rats, (A3) PI-IBS rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical analysis of intensity of SERT immunoreactivity is shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. **P < 0.01 versus normal rats (t-test).
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fig4: Effect of JCM-16021 on mucosal SERT expression in PI-IBS rats. (a) shows the immunofluorescence micrographs of (A1) negative control (primary antibody omitted) and the positive SERT expressions (arrowhead) in the mucosa of (A2) normal rats, (A3) PI-IBS rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical analysis of intensity of SERT immunoreactivity is shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. **P < 0.01 versus normal rats (t-test).

Mentions: As shown in Figure 4, the intensity of SERT immunoreactivity in colonic mucosa of PI-IBS rats was significantly decreased when compared to that of the control (40.3 ± 3.1 versus 49.0 ± 3.2; P < 0.05), suggesting that TNBS-induced PI-IBS rats also has the decreased SERT expression in the colon. After treatment of JCM-16021, there were no significant differences in the expression of SERT immunoreactive intensity between PI-IBS rats and JCM-16021-treated rats (40.3 ± 3.1 versus 42.6 ± 2.1; P < 0.05), suggesting that JCM-16021 treatment has little effect on the decreased SERT expression in PI-IBS rats.


JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Effect of JCM-16021 on mucosal SERT expression in PI-IBS rats. (a) shows the immunofluorescence micrographs of (A1) negative control (primary antibody omitted) and the positive SERT expressions (arrowhead) in the mucosa of (A2) normal rats, (A3) PI-IBS rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical analysis of intensity of SERT immunoreactivity is shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. **P < 0.01 versus normal rats (t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376539&req=5

fig4: Effect of JCM-16021 on mucosal SERT expression in PI-IBS rats. (a) shows the immunofluorescence micrographs of (A1) negative control (primary antibody omitted) and the positive SERT expressions (arrowhead) in the mucosa of (A2) normal rats, (A3) PI-IBS rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical analysis of intensity of SERT immunoreactivity is shown in (b). Data are shown as mean ± S.E.M., n = 5 per group. **P < 0.01 versus normal rats (t-test).
Mentions: As shown in Figure 4, the intensity of SERT immunoreactivity in colonic mucosa of PI-IBS rats was significantly decreased when compared to that of the control (40.3 ± 3.1 versus 49.0 ± 3.2; P < 0.05), suggesting that TNBS-induced PI-IBS rats also has the decreased SERT expression in the colon. After treatment of JCM-16021, there were no significant differences in the expression of SERT immunoreactive intensity between PI-IBS rats and JCM-16021-treated rats (40.3 ± 3.1 versus 42.6 ± 2.1; P < 0.05), suggesting that JCM-16021 treatment has little effect on the decreased SERT expression in PI-IBS rats.

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus