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JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus

Effects of JCM-16021 on enterochromaffin (EC) cell density and 5-HT content in the colon tissue of PI-IBS rats. (a) shows the representative EC cells (arrowhead) in colonic mucosa of the (A1) normal rats, (A2) PI-IBS rats, (A3) pCPA treated rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical graph of EC cell density is shown in (b), and 5-HT content in (c). Data are presented as mean ± S.E.M., n = 5 per group. #P < 0.05 versus normal rats, *P < 0.05, **P < 0.01 versus PI-IBS rats (t-test).
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fig2: Effects of JCM-16021 on enterochromaffin (EC) cell density and 5-HT content in the colon tissue of PI-IBS rats. (a) shows the representative EC cells (arrowhead) in colonic mucosa of the (A1) normal rats, (A2) PI-IBS rats, (A3) pCPA treated rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical graph of EC cell density is shown in (b), and 5-HT content in (c). Data are presented as mean ± S.E.M., n = 5 per group. #P < 0.05 versus normal rats, *P < 0.05, **P < 0.01 versus PI-IBS rats (t-test).

Mentions: As shown in Figure 2, the colonic EC cell density and 5-HT content were both significantly increased in PI-IBS rats (~51% in EC cell density, ~23% in 5-HT content) when compared to that of the control (P < 0.05), suggesting the occurrence of EC cell hyperplasia in PI-IBS rats. Compared with the PI-IBS rats, JCM-16021 treatment significantly and dose-dependently decreased the colonic EC cell density (20%~48%) and 5-HT content (8%~26%) in PI-IBS rats, suggesting that JCM-16021 can reduce colonic EC cells hyperplasia of PI-IBS rats. PCPA treatment also significantly decreased the colonic EC cell density and 5-HT content in PI-IBS rats (~57% in EC cell density, ~62.5% in 5-HT content, P < 0.05).


JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Effects of JCM-16021 on enterochromaffin (EC) cell density and 5-HT content in the colon tissue of PI-IBS rats. (a) shows the representative EC cells (arrowhead) in colonic mucosa of the (A1) normal rats, (A2) PI-IBS rats, (A3) pCPA treated rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical graph of EC cell density is shown in (b), and 5-HT content in (c). Data are presented as mean ± S.E.M., n = 5 per group. #P < 0.05 versus normal rats, *P < 0.05, **P < 0.01 versus PI-IBS rats (t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376539&req=5

fig2: Effects of JCM-16021 on enterochromaffin (EC) cell density and 5-HT content in the colon tissue of PI-IBS rats. (a) shows the representative EC cells (arrowhead) in colonic mucosa of the (A1) normal rats, (A2) PI-IBS rats, (A3) pCPA treated rats, and (A4) high dose JCM-16021 treated rats (Scale bar, 200 μm). Statistical graph of EC cell density is shown in (b), and 5-HT content in (c). Data are presented as mean ± S.E.M., n = 5 per group. #P < 0.05 versus normal rats, *P < 0.05, **P < 0.01 versus PI-IBS rats (t-test).
Mentions: As shown in Figure 2, the colonic EC cell density and 5-HT content were both significantly increased in PI-IBS rats (~51% in EC cell density, ~23% in 5-HT content) when compared to that of the control (P < 0.05), suggesting the occurrence of EC cell hyperplasia in PI-IBS rats. Compared with the PI-IBS rats, JCM-16021 treatment significantly and dose-dependently decreased the colonic EC cell density (20%~48%) and 5-HT content (8%~26%) in PI-IBS rats, suggesting that JCM-16021 can reduce colonic EC cells hyperplasia of PI-IBS rats. PCPA treatment also significantly decreased the colonic EC cell density and 5-HT content in PI-IBS rats (~57% in EC cell density, ~62.5% in 5-HT content, P < 0.05).

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus