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JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus

Effects of JCM-16021 on visceral hypersensitivity of PI-IBS rats. (a) depicts the analgesic effect of JCM-16021 in PI-IBS rats in terms of pain threshold pressure, while (b) depicts the effect of JCM-16021 on visceral motor response to graded CRD in PI-IBS rats. The representative EMG graphs from normal rats, PI-IBS rats, and high-dose JCM-16021 treated rats in a 20 s noxious (80 mmHg) colorectal distension period are shown in (c). Data are presented as mean ± S.E.M., n = 5 per group in AWR test, n = 8 per group in EMG recording test. #P < 0.05 versus normal rats, *P < 0.05, versus PI-IBS rats (t-test).
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fig1: Effects of JCM-16021 on visceral hypersensitivity of PI-IBS rats. (a) depicts the analgesic effect of JCM-16021 in PI-IBS rats in terms of pain threshold pressure, while (b) depicts the effect of JCM-16021 on visceral motor response to graded CRD in PI-IBS rats. The representative EMG graphs from normal rats, PI-IBS rats, and high-dose JCM-16021 treated rats in a 20 s noxious (80 mmHg) colorectal distension period are shown in (c). Data are presented as mean ± S.E.M., n = 5 per group in AWR test, n = 8 per group in EMG recording test. #P < 0.05 versus normal rats, *P < 0.05, versus PI-IBS rats (t-test).

Mentions: As shown in Figure 1(a), the pain threshold pressures in PI-IBS rats (TNBS + water treated rats) were significantly decreased when compared to that of the control (saline + water treated rats, P < 0.05). After pCPA treatment, the pain threshold pressures in PI-IBS rats were significantly elevated (P < 0.05), suggesting that 5-HT played an important role in the development of visceral hypersensitivity. After JCM-16021 treatment, the pain threshold pressures were significantly and dose-dependently elevated when compared to that of the PI-IBS rats (P < 0.05), indicating that JCM-16021 has analgesic effect in PI-IBS rats. Consistent with the findings from AWR test, the results from EMG recording (Figures 1(b) and 1(c)) also showed that visceral motor responses to graded CRD in PI-IBS rats were significantly increased when compared to that of the control (P < 0.05). After JCM-16021 treatment, the visceral motor responses to graded CRD in PI-IBS rats were decreased significantly in a dose-dependent manner (P < 0.05).


JCM-16021, a Chinese Herbal Formula, Attenuated Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome through Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in Rats.

Qin HY, Xiao HT, Leung FP, Yang ZJ, Wu JC, Sung JJ, Xu HX, Tong XD, Bian ZX - Evid Based Complement Alternat Med (2012)

Effects of JCM-16021 on visceral hypersensitivity of PI-IBS rats. (a) depicts the analgesic effect of JCM-16021 in PI-IBS rats in terms of pain threshold pressure, while (b) depicts the effect of JCM-16021 on visceral motor response to graded CRD in PI-IBS rats. The representative EMG graphs from normal rats, PI-IBS rats, and high-dose JCM-16021 treated rats in a 20 s noxious (80 mmHg) colorectal distension period are shown in (c). Data are presented as mean ± S.E.M., n = 5 per group in AWR test, n = 8 per group in EMG recording test. #P < 0.05 versus normal rats, *P < 0.05, versus PI-IBS rats (t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376539&req=5

fig1: Effects of JCM-16021 on visceral hypersensitivity of PI-IBS rats. (a) depicts the analgesic effect of JCM-16021 in PI-IBS rats in terms of pain threshold pressure, while (b) depicts the effect of JCM-16021 on visceral motor response to graded CRD in PI-IBS rats. The representative EMG graphs from normal rats, PI-IBS rats, and high-dose JCM-16021 treated rats in a 20 s noxious (80 mmHg) colorectal distension period are shown in (c). Data are presented as mean ± S.E.M., n = 5 per group in AWR test, n = 8 per group in EMG recording test. #P < 0.05 versus normal rats, *P < 0.05, versus PI-IBS rats (t-test).
Mentions: As shown in Figure 1(a), the pain threshold pressures in PI-IBS rats (TNBS + water treated rats) were significantly decreased when compared to that of the control (saline + water treated rats, P < 0.05). After pCPA treatment, the pain threshold pressures in PI-IBS rats were significantly elevated (P < 0.05), suggesting that 5-HT played an important role in the development of visceral hypersensitivity. After JCM-16021 treatment, the pain threshold pressures were significantly and dose-dependently elevated when compared to that of the PI-IBS rats (P < 0.05), indicating that JCM-16021 has analgesic effect in PI-IBS rats. Consistent with the findings from AWR test, the results from EMG recording (Figures 1(b) and 1(c)) also showed that visceral motor responses to graded CRD in PI-IBS rats were significantly increased when compared to that of the control (P < 0.05). After JCM-16021 treatment, the visceral motor responses to graded CRD in PI-IBS rats were decreased significantly in a dose-dependent manner (P < 0.05).

Bottom Line: Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated.Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment.These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

ABSTRACT
The present study aimed to investigate the analgesic effect of JCM-16021, a revised traditional Chinese herbal formula, on postinflammatory irritable bowel syndrome (PI-IBS) in rats. The trinitrobenzene sulfonic (TNBS) acid-induced PI-IBS model rats were orally administrated with different doses of JCM-16021 (1.2, 2.4, and 4.8 g/kg/d) for 14 consecutive days. The results showed that JCM-16021 treatment dose-dependently attenuated visceral hyperalgesia in PI-IBS rats. Further, the colonic enterochromaffin (EC) cell number, serotonin (5-HT) content, tryptophan hydroxylase expression, and mechanical-stimuli-induced 5-HT release were significantly ameliorated. Moreover, the decreased levels of mucosal cytokines in PI-IBS, especially the helper T-cell type 1- (T(h)1-) related cytokine TNF-α, were also elevated after JCM-16021 treatment. These data demonstrate that the analgesic effect of JCM-16021 on TNBS-induced PI-IBS rats may be medicated via reducing colonic EC cell hyperplasia and 5-HT availability.

No MeSH data available.


Related in: MedlinePlus