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Photosensitive-polyimide based method for fabricating various neural electrode architectures.

Kato YX, Furukawa S, Samejima K, Hironaka N, Kashino M - Front Neuroeng (2012)

Bottom Line: After characterizing PSPI's properties for micromachining processes, we successfully designed and fabricated various neural electrodes even on a non-flat substrate using only one PSPI as an insulation material and without the time-consuming dry etching processes.In vivo neural recordings using anesthetized rats demonstrated that these electrodes can be used to record neural activities repeatedly without any breakage and mechanical failures, which potentially promises stable recordings for long periods of time.These successes make us believe that this PSPI-based fabrication is a powerful method, permitting flexible design, and easy optimization of electrode architectures for a variety of electrophysiological experimental research with improved neural recording performance.

View Article: PubMed Central - PubMed

Affiliation: Brain Science Institute, Tamagawa University, Machida Tokyo, Japan.

ABSTRACT
An extensive photosensitive-polyimide (PSPI)-based method for designing and fabricating various neural electrode architectures was developed. The method aims to broaden the design flexibility and expand the fabrication capability for neural electrodes to improve the quality of recorded signals and integrate other functions. After characterizing PSPI's properties for micromachining processes, we successfully designed and fabricated various neural electrodes even on a non-flat substrate using only one PSPI as an insulation material and without the time-consuming dry etching processes. The fabricated neural electrodes were an electrocorticogram (ECoG) electrode, a mesh intracortical electrode with a unique lattice-like mesh structure to fixate neural tissue, and a guide cannula electrode with recording microelectrodes placed on the curved surface of a guide cannula as a microdialysis probe. In vivo neural recordings using anesthetized rats demonstrated that these electrodes can be used to record neural activities repeatedly without any breakage and mechanical failures, which potentially promises stable recordings for long periods of time. These successes make us believe that this PSPI-based fabrication is a powerful method, permitting flexible design, and easy optimization of electrode architectures for a variety of electrophysiological experimental research with improved neural recording performance.

No MeSH data available.


AEP recorded from the auditory cortex of an anesthetized rat by the guide cannula electrode of the microdialysis electrode. The trace shown here is averaged AEP across 100 trials. Auditory stimuli were 100 ms tone bursts of 10 kHz at 80 dB SPL.
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Figure 8: AEP recorded from the auditory cortex of an anesthetized rat by the guide cannula electrode of the microdialysis electrode. The trace shown here is averaged AEP across 100 trials. Auditory stimuli were 100 ms tone bursts of 10 kHz at 80 dB SPL.

Mentions: We also succeeded in recording neural signals with the mesh intracortical electrode. Figure 7 shows the recorded spontaneous activities. The inset shows spike waveforms that were detected with our spike-sorting software (see In vivo Neural Recordins in Methods) and had an SNR higher than 4. The spikes had durations of about 1 ms and amplitudes of 96 ± 9.0 (SD) μV, corresponding to SNRs of 4.7 ± 0.44 (SD). The SNR values fell to a lower range than those reported previously (4–7; Nordhausen et al., 1996; Vetter et al., 2004), probably because the recording microelectrode impedance was lower than that in the previous reports. In the recording experiment with the guide cannula electrode, the AEPs were also successfully recorded from the auditory cortex. Figure 8 shows an averaged AEP in response to the tone burst stimuli.


Photosensitive-polyimide based method for fabricating various neural electrode architectures.

Kato YX, Furukawa S, Samejima K, Hironaka N, Kashino M - Front Neuroeng (2012)

AEP recorded from the auditory cortex of an anesthetized rat by the guide cannula electrode of the microdialysis electrode. The trace shown here is averaged AEP across 100 trials. Auditory stimuli were 100 ms tone bursts of 10 kHz at 80 dB SPL.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3376501&req=5

Figure 8: AEP recorded from the auditory cortex of an anesthetized rat by the guide cannula electrode of the microdialysis electrode. The trace shown here is averaged AEP across 100 trials. Auditory stimuli were 100 ms tone bursts of 10 kHz at 80 dB SPL.
Mentions: We also succeeded in recording neural signals with the mesh intracortical electrode. Figure 7 shows the recorded spontaneous activities. The inset shows spike waveforms that were detected with our spike-sorting software (see In vivo Neural Recordins in Methods) and had an SNR higher than 4. The spikes had durations of about 1 ms and amplitudes of 96 ± 9.0 (SD) μV, corresponding to SNRs of 4.7 ± 0.44 (SD). The SNR values fell to a lower range than those reported previously (4–7; Nordhausen et al., 1996; Vetter et al., 2004), probably because the recording microelectrode impedance was lower than that in the previous reports. In the recording experiment with the guide cannula electrode, the AEPs were also successfully recorded from the auditory cortex. Figure 8 shows an averaged AEP in response to the tone burst stimuli.

Bottom Line: After characterizing PSPI's properties for micromachining processes, we successfully designed and fabricated various neural electrodes even on a non-flat substrate using only one PSPI as an insulation material and without the time-consuming dry etching processes.In vivo neural recordings using anesthetized rats demonstrated that these electrodes can be used to record neural activities repeatedly without any breakage and mechanical failures, which potentially promises stable recordings for long periods of time.These successes make us believe that this PSPI-based fabrication is a powerful method, permitting flexible design, and easy optimization of electrode architectures for a variety of electrophysiological experimental research with improved neural recording performance.

View Article: PubMed Central - PubMed

Affiliation: Brain Science Institute, Tamagawa University, Machida Tokyo, Japan.

ABSTRACT
An extensive photosensitive-polyimide (PSPI)-based method for designing and fabricating various neural electrode architectures was developed. The method aims to broaden the design flexibility and expand the fabrication capability for neural electrodes to improve the quality of recorded signals and integrate other functions. After characterizing PSPI's properties for micromachining processes, we successfully designed and fabricated various neural electrodes even on a non-flat substrate using only one PSPI as an insulation material and without the time-consuming dry etching processes. The fabricated neural electrodes were an electrocorticogram (ECoG) electrode, a mesh intracortical electrode with a unique lattice-like mesh structure to fixate neural tissue, and a guide cannula electrode with recording microelectrodes placed on the curved surface of a guide cannula as a microdialysis probe. In vivo neural recordings using anesthetized rats demonstrated that these electrodes can be used to record neural activities repeatedly without any breakage and mechanical failures, which potentially promises stable recordings for long periods of time. These successes make us believe that this PSPI-based fabrication is a powerful method, permitting flexible design, and easy optimization of electrode architectures for a variety of electrophysiological experimental research with improved neural recording performance.

No MeSH data available.