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Generation of biotechnology-derived Flavobacterium columnare ghosts by PhiX174 gene E-mediated inactivation and the potential as vaccine candidates against infection in grass carp.

Zhu W, Yang G, Zhang Y, Yuan J, An L - J. Biomed. Biotechnol. (2012)

Bottom Line: Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS.Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%).These results showed that FCG could confer immune protection against F. columnare infection.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Animal Resistance Biology of Shandong, College of Life Sciences, Shandong Normal University, Jinan 250014, China.

ABSTRACT
Flavobacterium columnare is a bacterial pathogen causing high mortality rates for many freshwater fish species. Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease. Here, in order to produce bacterial ghost vaccine, a specific Flavobacterium lysis plasmid pBV-E-cat was constructed by cloning PhiX174 lysis gene E and the cat gene with the promoter of F. columnare into the prokaryotic expression vector pBV220. The plasmid was successfully electroporated into the strain F. columnare G4cpN22 after curing of its endogenous plasmid. F. columnare G4cpN22 ghosts (FCGs) were generated for the first time by gene E-mediated lysis, and the vaccine potential of FCG was investigated in grass carp (Ctenopharyngodon idellus) by intraperitoneal route. Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS. Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%). These results showed that FCG could confer immune protection against F. columnare infection. As a nonliving whole cell envelope preparation, FCG may provide an ideal alternative to pathogen-based vaccines against columnaris in aquaculture.

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Related in: MedlinePlus

Serum bactericidal activities of grass carp vaccinated with FCG or FKC. The grass carp were vaccinated with FCG or FKC via intraperitoneal injection with a boost at 4 weeks after primary immunization. Serum bactericidal activities of FCG group were significantly higher than FKC group at the examined time points of postimmunization (P < 0.01).
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fig5: Serum bactericidal activities of grass carp vaccinated with FCG or FKC. The grass carp were vaccinated with FCG or FKC via intraperitoneal injection with a boost at 4 weeks after primary immunization. Serum bactericidal activities of FCG group were significantly higher than FKC group at the examined time points of postimmunization (P < 0.01).

Mentions: Serum bactericidal activity was the lowest in PBS group and the highest in FCG group. Serum bactericidal activity of FCG group was elevated significantly above FKC group and PBS control group at all times postimmunization (P < 0.01) (Figure 5). The viable bacterial counts (41%) were closely 2-fold lower in FCG group compared with FKC group (80%) at 2 weeks after booster immunization (6 weeks).


Generation of biotechnology-derived Flavobacterium columnare ghosts by PhiX174 gene E-mediated inactivation and the potential as vaccine candidates against infection in grass carp.

Zhu W, Yang G, Zhang Y, Yuan J, An L - J. Biomed. Biotechnol. (2012)

Serum bactericidal activities of grass carp vaccinated with FCG or FKC. The grass carp were vaccinated with FCG or FKC via intraperitoneal injection with a boost at 4 weeks after primary immunization. Serum bactericidal activities of FCG group were significantly higher than FKC group at the examined time points of postimmunization (P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376489&req=5

fig5: Serum bactericidal activities of grass carp vaccinated with FCG or FKC. The grass carp were vaccinated with FCG or FKC via intraperitoneal injection with a boost at 4 weeks after primary immunization. Serum bactericidal activities of FCG group were significantly higher than FKC group at the examined time points of postimmunization (P < 0.01).
Mentions: Serum bactericidal activity was the lowest in PBS group and the highest in FCG group. Serum bactericidal activity of FCG group was elevated significantly above FKC group and PBS control group at all times postimmunization (P < 0.01) (Figure 5). The viable bacterial counts (41%) were closely 2-fold lower in FCG group compared with FKC group (80%) at 2 weeks after booster immunization (6 weeks).

Bottom Line: Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS.Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%).These results showed that FCG could confer immune protection against F. columnare infection.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Animal Resistance Biology of Shandong, College of Life Sciences, Shandong Normal University, Jinan 250014, China.

ABSTRACT
Flavobacterium columnare is a bacterial pathogen causing high mortality rates for many freshwater fish species. Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease. Here, in order to produce bacterial ghost vaccine, a specific Flavobacterium lysis plasmid pBV-E-cat was constructed by cloning PhiX174 lysis gene E and the cat gene with the promoter of F. columnare into the prokaryotic expression vector pBV220. The plasmid was successfully electroporated into the strain F. columnare G4cpN22 after curing of its endogenous plasmid. F. columnare G4cpN22 ghosts (FCGs) were generated for the first time by gene E-mediated lysis, and the vaccine potential of FCG was investigated in grass carp (Ctenopharyngodon idellus) by intraperitoneal route. Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS. Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%). These results showed that FCG could confer immune protection against F. columnare infection. As a nonliving whole cell envelope preparation, FCG may provide an ideal alternative to pathogen-based vaccines against columnaris in aquaculture.

Show MeSH
Related in: MedlinePlus