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The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway.

Lin YL, Tsay HJ, Liao YF, Wu MF, Wang CN, Shiao YJ - Evid Based Complement Alternat Med (2012)

Bottom Line: Therefore, the effects of F. macrophylla on Aβ production and degradation were studied.The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells).The effects on Aβ degradation were evaluated on a cell-free system.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicinal Chemistry, National Research Institute of Chinese Medicine, Taipei 112, Taiwan.

ABSTRACT
Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer's disease. Therefore, the effects of F. macrophylla on Aβ production and degradation were studied. The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aβ degradation were evaluated on a cell-free system. An ELISA assay was applied to detect the level of Aβ1-40 and Aβ1-42. Western blots assay was employed to measure the levels of soluble amyloid precursor protein and insulin degrading enzyme (IDE). Three fractions of F. macrophylla modified Aβ accumulation by both inhibiting β-secretase and activating IDE. Three flavonoids modified Aβ accumulation by activating IDE. The activated IDE pool by the flavonoids was distinctly regulated by bacitracin (an IDE inhibitor). Furthermore, flavonoid 94-18-13 also modulates Aβ accumulation by enhancing IDE expression. In conclusion, the components of F. macrophylla possess the potential for developing new therapeutic drugs for Alzheimer's disease.

No MeSH data available.


Related in: MedlinePlus

The effects of the fractions and flavonoids of F. macrophylla on Aβ1-40 degradation in the N2a-conditioned medium. Aβ1-40 (10 ng) were incubated in the N2a-conditioned medium with 100 nM insulin and the fractions and flavonoids of F. macrophylla at NTC, 37°C for 20 h. The level of remaining Aβ1-40 was determined by ELISA. Results are means ± SD from three independent experiments. Significant differences between control and the treated cells are indicated by **P < 0.01, ***P < 0.001.
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fig4: The effects of the fractions and flavonoids of F. macrophylla on Aβ1-40 degradation in the N2a-conditioned medium. Aβ1-40 (10 ng) were incubated in the N2a-conditioned medium with 100 nM insulin and the fractions and flavonoids of F. macrophylla at NTC, 37°C for 20 h. The level of remaining Aβ1-40 was determined by ELISA. Results are means ± SD from three independent experiments. Significant differences between control and the treated cells are indicated by **P < 0.01, ***P < 0.001.

Mentions: The fraction EtOH, EA-47, B50M, and flavonoid 49-3, 52-11, and 94-19-13 decreased the remaining synthetic Aβ1-40 to 82.66 ± 1.26%, 83.25 ± 0.74%, 83.50 ± 7.30%, 76.02 ± 4.88%, 83.24 ± 8.60%, and 82.31 ± 8.04% of the control, respectively (Figure 4). The results suggesting that the fractions and flavonoids may ameliorate Aβ accumulation by promoting Aβ degradation. The similar effects were found on Aβ1-42 (data not shown).


The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway.

Lin YL, Tsay HJ, Liao YF, Wu MF, Wang CN, Shiao YJ - Evid Based Complement Alternat Med (2012)

The effects of the fractions and flavonoids of F. macrophylla on Aβ1-40 degradation in the N2a-conditioned medium. Aβ1-40 (10 ng) were incubated in the N2a-conditioned medium with 100 nM insulin and the fractions and flavonoids of F. macrophylla at NTC, 37°C for 20 h. The level of remaining Aβ1-40 was determined by ELISA. Results are means ± SD from three independent experiments. Significant differences between control and the treated cells are indicated by **P < 0.01, ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376484&req=5

fig4: The effects of the fractions and flavonoids of F. macrophylla on Aβ1-40 degradation in the N2a-conditioned medium. Aβ1-40 (10 ng) were incubated in the N2a-conditioned medium with 100 nM insulin and the fractions and flavonoids of F. macrophylla at NTC, 37°C for 20 h. The level of remaining Aβ1-40 was determined by ELISA. Results are means ± SD from three independent experiments. Significant differences between control and the treated cells are indicated by **P < 0.01, ***P < 0.001.
Mentions: The fraction EtOH, EA-47, B50M, and flavonoid 49-3, 52-11, and 94-19-13 decreased the remaining synthetic Aβ1-40 to 82.66 ± 1.26%, 83.25 ± 0.74%, 83.50 ± 7.30%, 76.02 ± 4.88%, 83.24 ± 8.60%, and 82.31 ± 8.04% of the control, respectively (Figure 4). The results suggesting that the fractions and flavonoids may ameliorate Aβ accumulation by promoting Aβ degradation. The similar effects were found on Aβ1-42 (data not shown).

Bottom Line: Therefore, the effects of F. macrophylla on Aβ production and degradation were studied.The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells).The effects on Aβ degradation were evaluated on a cell-free system.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicinal Chemistry, National Research Institute of Chinese Medicine, Taipei 112, Taiwan.

ABSTRACT
Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer's disease. Therefore, the effects of F. macrophylla on Aβ production and degradation were studied. The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aβ degradation were evaluated on a cell-free system. An ELISA assay was applied to detect the level of Aβ1-40 and Aβ1-42. Western blots assay was employed to measure the levels of soluble amyloid precursor protein and insulin degrading enzyme (IDE). Three fractions of F. macrophylla modified Aβ accumulation by both inhibiting β-secretase and activating IDE. Three flavonoids modified Aβ accumulation by activating IDE. The activated IDE pool by the flavonoids was distinctly regulated by bacitracin (an IDE inhibitor). Furthermore, flavonoid 94-18-13 also modulates Aβ accumulation by enhancing IDE expression. In conclusion, the components of F. macrophylla possess the potential for developing new therapeutic drugs for Alzheimer's disease.

No MeSH data available.


Related in: MedlinePlus