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The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway.

Lin YL, Tsay HJ, Liao YF, Wu MF, Wang CN, Shiao YJ - Evid Based Complement Alternat Med (2012)

Bottom Line: Therefore, the effects of F. macrophylla on Aβ production and degradation were studied.The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells).The effects on Aβ degradation were evaluated on a cell-free system.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicinal Chemistry, National Research Institute of Chinese Medicine, Taipei 112, Taiwan.

ABSTRACT
Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer's disease. Therefore, the effects of F. macrophylla on Aβ production and degradation were studied. The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aβ degradation were evaluated on a cell-free system. An ELISA assay was applied to detect the level of Aβ1-40 and Aβ1-42. Western blots assay was employed to measure the levels of soluble amyloid precursor protein and insulin degrading enzyme (IDE). Three fractions of F. macrophylla modified Aβ accumulation by both inhibiting β-secretase and activating IDE. Three flavonoids modified Aβ accumulation by activating IDE. The activated IDE pool by the flavonoids was distinctly regulated by bacitracin (an IDE inhibitor). Furthermore, flavonoid 94-18-13 also modulates Aβ accumulation by enhancing IDE expression. In conclusion, the components of F. macrophylla possess the potential for developing new therapeutic drugs for Alzheimer's disease.

No MeSH data available.


Related in: MedlinePlus

The structure and chemical name of the flavonoids isolated from F. macrophylla.
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Related In: Results  -  Collection


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fig1: The structure and chemical name of the flavonoids isolated from F. macrophylla.

Mentions: The aerial parts of F. macrophylla were collected from Kaohsiung County, Taiwan in May, 2002. The plant was identified by Mr. Jun-Chih Ou, former associate investigator of National Research Institute of Chinese Medicine, and comparison with the voucher specimens was deposited earlier at the Herbarium of the Department of Botany, National Taiwan University, Taipei, Taiwan (no. TAI219262, April, 1988). The extraction and isolation of each fraction for this assay is listed in Table 1, and the structure and chemical name of the flavonoids isolated from F. macrophylla were displayed in Figure 1.


The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway.

Lin YL, Tsay HJ, Liao YF, Wu MF, Wang CN, Shiao YJ - Evid Based Complement Alternat Med (2012)

The structure and chemical name of the flavonoids isolated from F. macrophylla.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376484&req=5

fig1: The structure and chemical name of the flavonoids isolated from F. macrophylla.
Mentions: The aerial parts of F. macrophylla were collected from Kaohsiung County, Taiwan in May, 2002. The plant was identified by Mr. Jun-Chih Ou, former associate investigator of National Research Institute of Chinese Medicine, and comparison with the voucher specimens was deposited earlier at the Herbarium of the Department of Botany, National Taiwan University, Taipei, Taiwan (no. TAI219262, April, 1988). The extraction and isolation of each fraction for this assay is listed in Table 1, and the structure and chemical name of the flavonoids isolated from F. macrophylla were displayed in Figure 1.

Bottom Line: Therefore, the effects of F. macrophylla on Aβ production and degradation were studied.The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells).The effects on Aβ degradation were evaluated on a cell-free system.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicinal Chemistry, National Research Institute of Chinese Medicine, Taipei 112, Taiwan.

ABSTRACT
Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer's disease. Therefore, the effects of F. macrophylla on Aβ production and degradation were studied. The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aβ degradation were evaluated on a cell-free system. An ELISA assay was applied to detect the level of Aβ1-40 and Aβ1-42. Western blots assay was employed to measure the levels of soluble amyloid precursor protein and insulin degrading enzyme (IDE). Three fractions of F. macrophylla modified Aβ accumulation by both inhibiting β-secretase and activating IDE. Three flavonoids modified Aβ accumulation by activating IDE. The activated IDE pool by the flavonoids was distinctly regulated by bacitracin (an IDE inhibitor). Furthermore, flavonoid 94-18-13 also modulates Aβ accumulation by enhancing IDE expression. In conclusion, the components of F. macrophylla possess the potential for developing new therapeutic drugs for Alzheimer's disease.

No MeSH data available.


Related in: MedlinePlus