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Gel-based and gel-free identification of proteins and phosphopeptides during egg-to-larva transition in polychaete Neanthes arenaceodentata.

Chandramouli KH, Reish D, Qian PY - PLoS ONE (2012)

Bottom Line: Sixty percent of the identified proteins were related to structural reorganization and others with protein synthesis, stress response and attachment.This is the first report on changes in protein expression and phosphorylation sites at Thr/Ser in early development of N. arenaceodentata.The 2-DE proteome maps and identified phosphoproteins contributes toward understanding the state of fertilized ova and early larval stages and serves as a basis for further studies on proteomics changes under different developmental conditions in this and other polychaete species.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, China.

ABSTRACT
The polychaete Neanthes arenaceodentata- is cosmopolitan in distribution-, has been used as a laboratory test animal. Life history of this species has several unique features; the female dies after spawning and the male incubates the fertilized eggs through the 21-segmented stage. The larvae leave the tube and commence feeding. Changes in protein abundance and phosphorylation were examined during early development of N. arenaceodentata. A gel-based approach and gel-free enrichment of phosphopeptides coupled with mass spectrometry were used to identify proteins and phosphopeptides in fertilized ova and larval stages. Patterns of proteins and phosphoproteins changed from fertilized ova to larval stages. Twelve proteins occurred in phosphorylated form and nine as stage specific proteins. Cytoskeletal proteins have exhibited differential phosphorylation from ova to larval stages; whereas, other proteins exhibited stage-specific phosphorylation patterns. Ten phosphopeptides were identified that showed phosphorylation sites on serine or threonine residues. Sixty percent of the identified proteins were related to structural reorganization and others with protein synthesis, stress response and attachment. The abundance and distribution of two cytoskeleton proteins were examined further by 2-DE Western blot analysis. This is the first report on changes in protein expression and phosphorylation sites at Thr/Ser in early development of N. arenaceodentata. The 2-DE proteome maps and identified phosphoproteins contributes toward understanding the state of fertilized ova and early larval stages and serves as a basis for further studies on proteomics changes under different developmental conditions in this and other polychaete species.

Show MeSH
Early developmental stages of the polychaete Neanthes arenaceodentata.Three developmental stages were chosen for proteomic analysis: Fertilized ova, 3–4 segmented early larva and 10–12 segmented old larva. Drawings: Three segmented larva and twelve segmented larva.
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pone-0038814-g001: Early developmental stages of the polychaete Neanthes arenaceodentata.Three developmental stages were chosen for proteomic analysis: Fertilized ova, 3–4 segmented early larva and 10–12 segmented old larva. Drawings: Three segmented larva and twelve segmented larva.

Mentions: The polychaete used in this study belongs to a species complex which is cosmopolitan in distribution. The California population is referred to as N.arenaceodentata and has been used as a toxicological test animal [1]. Specimens were taken from a laboratory population maintained at California State University, Long Beach. The culture was established in 1964 from 6 specimens collected from Los Angeles Harbor. No additional specimens have been introduced into the population which has undergone over 200 generations. Specimens from several matings were removed from tubes at fertilization, fertilized ova, 3–4 segmented early larvae and 10–12 segmented old larvae (Fig. 1) were shipped frozen to Hong Kong by overnight express.


Gel-based and gel-free identification of proteins and phosphopeptides during egg-to-larva transition in polychaete Neanthes arenaceodentata.

Chandramouli KH, Reish D, Qian PY - PLoS ONE (2012)

Early developmental stages of the polychaete Neanthes arenaceodentata.Three developmental stages were chosen for proteomic analysis: Fertilized ova, 3–4 segmented early larva and 10–12 segmented old larva. Drawings: Three segmented larva and twelve segmented larva.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376139&req=5

pone-0038814-g001: Early developmental stages of the polychaete Neanthes arenaceodentata.Three developmental stages were chosen for proteomic analysis: Fertilized ova, 3–4 segmented early larva and 10–12 segmented old larva. Drawings: Three segmented larva and twelve segmented larva.
Mentions: The polychaete used in this study belongs to a species complex which is cosmopolitan in distribution. The California population is referred to as N.arenaceodentata and has been used as a toxicological test animal [1]. Specimens were taken from a laboratory population maintained at California State University, Long Beach. The culture was established in 1964 from 6 specimens collected from Los Angeles Harbor. No additional specimens have been introduced into the population which has undergone over 200 generations. Specimens from several matings were removed from tubes at fertilization, fertilized ova, 3–4 segmented early larvae and 10–12 segmented old larvae (Fig. 1) were shipped frozen to Hong Kong by overnight express.

Bottom Line: Sixty percent of the identified proteins were related to structural reorganization and others with protein synthesis, stress response and attachment.This is the first report on changes in protein expression and phosphorylation sites at Thr/Ser in early development of N. arenaceodentata.The 2-DE proteome maps and identified phosphoproteins contributes toward understanding the state of fertilized ova and early larval stages and serves as a basis for further studies on proteomics changes under different developmental conditions in this and other polychaete species.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, China.

ABSTRACT
The polychaete Neanthes arenaceodentata- is cosmopolitan in distribution-, has been used as a laboratory test animal. Life history of this species has several unique features; the female dies after spawning and the male incubates the fertilized eggs through the 21-segmented stage. The larvae leave the tube and commence feeding. Changes in protein abundance and phosphorylation were examined during early development of N. arenaceodentata. A gel-based approach and gel-free enrichment of phosphopeptides coupled with mass spectrometry were used to identify proteins and phosphopeptides in fertilized ova and larval stages. Patterns of proteins and phosphoproteins changed from fertilized ova to larval stages. Twelve proteins occurred in phosphorylated form and nine as stage specific proteins. Cytoskeletal proteins have exhibited differential phosphorylation from ova to larval stages; whereas, other proteins exhibited stage-specific phosphorylation patterns. Ten phosphopeptides were identified that showed phosphorylation sites on serine or threonine residues. Sixty percent of the identified proteins were related to structural reorganization and others with protein synthesis, stress response and attachment. The abundance and distribution of two cytoskeleton proteins were examined further by 2-DE Western blot analysis. This is the first report on changes in protein expression and phosphorylation sites at Thr/Ser in early development of N. arenaceodentata. The 2-DE proteome maps and identified phosphoproteins contributes toward understanding the state of fertilized ova and early larval stages and serves as a basis for further studies on proteomics changes under different developmental conditions in this and other polychaete species.

Show MeSH