Limits...
DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients.

Fitting C, Parlato M, Adib-Conquy M, Memain N, Philippart F, Misset B, Monchi M, Cavaillon JM, Adrie C - PLoS ONE (2012)

Bottom Line: Of these, 51.4% were positive using the VYOO® test.The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%.This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

View Article: PubMed Central - PubMed

Affiliation: Unit Cytokines & Inflammation, Department of Infection and Epidemiology, Institut Pasteur, Paris, France.

ABSTRACT
Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOO®) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) for pathogen detection provided by SIRS-Lab (Jena, Germany). We analyzed 72 sepsis patients and 14 non-infectious systemic inflammatory response syndrome (SIRS) patients. Among the sepsis patients, 20 had a positive blood culture and 35 had a positive microbiology in other biological samples. Of these, 51.4% were positive using the VYOO® test. Among the sepsis patients with a negative microbiology and the non-infectious SIRS, 29.4% and 14.2% were positive with the VYOO® test, respectively. The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%. This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

Show MeSH

Related in: MedlinePlus

Matching between microbiology and bacterial DNA analysis.Comparison of the bacterial identification by classical microbiological analysis or by the VYOO® technology. White bars: identification by classical microbiology in any compartments; grey bars: identification by VYOO® test in blood samples; black bars: matching between microbiology and VYOO® test.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3376137&req=5

pone-0038916-g002: Matching between microbiology and bacterial DNA analysis.Comparison of the bacterial identification by classical microbiological analysis or by the VYOO® technology. White bars: identification by classical microbiology in any compartments; grey bars: identification by VYOO® test in blood samples; black bars: matching between microbiology and VYOO® test.

Mentions: Microorganisms were detected in the blood of 37 out of 72 sepsis patients (51.4%) with the VYOO® technique and the results were better amongst those with a positive blood culture: 14/20 (70%) were accurately detected (table 2). Interestingly, 9 patients with identified organisms at the site of infection were correctly detected with the VYOO® technique without concomitant septicaemia. For two additional positive PCR in the septic cohort, the microorganism detected was compatible with non-documented sites of infection amongst these patients (Pneumonia/Streptococcus pneumoniae and severe colitis/Morganella morganii). Two positive VYOO® detections were obtained among the non-infectious SIRS cohort (14,2%), neither of which had a documented infection based on classical bacteriological tests. No fungal infections were detected using the VYOO® technology while microbiology revealed one positive blood culture (C. albicans) and positivity in five other samples (1 peritonitis, 3 lung infections, 1 surgical site). According to microbiology, 48% of the patients were infected with Gram-negative bacteria, among whom 28% were revealed by the VYOO® technology. Among the pathogens revealed by microbiological analysis and the VYOO® technology, E. coli was the most prominent Gram-negative bacteria (34,6% and 19,6% respectively) (figure 2). Among Gram-positive-bacteria the frequency of Staphylococcus aureus (incl. MRSA) was 22.7% according to microbiological analysis and 30.8% according to the VYOO® technology. The correspondence in bacterial identification between microbiology and the VYOO® technology was 46.2% (figure 2).


DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients.

Fitting C, Parlato M, Adib-Conquy M, Memain N, Philippart F, Misset B, Monchi M, Cavaillon JM, Adrie C - PLoS ONE (2012)

Matching between microbiology and bacterial DNA analysis.Comparison of the bacterial identification by classical microbiological analysis or by the VYOO® technology. White bars: identification by classical microbiology in any compartments; grey bars: identification by VYOO® test in blood samples; black bars: matching between microbiology and VYOO® test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376137&req=5

pone-0038916-g002: Matching between microbiology and bacterial DNA analysis.Comparison of the bacterial identification by classical microbiological analysis or by the VYOO® technology. White bars: identification by classical microbiology in any compartments; grey bars: identification by VYOO® test in blood samples; black bars: matching between microbiology and VYOO® test.
Mentions: Microorganisms were detected in the blood of 37 out of 72 sepsis patients (51.4%) with the VYOO® technique and the results were better amongst those with a positive blood culture: 14/20 (70%) were accurately detected (table 2). Interestingly, 9 patients with identified organisms at the site of infection were correctly detected with the VYOO® technique without concomitant septicaemia. For two additional positive PCR in the septic cohort, the microorganism detected was compatible with non-documented sites of infection amongst these patients (Pneumonia/Streptococcus pneumoniae and severe colitis/Morganella morganii). Two positive VYOO® detections were obtained among the non-infectious SIRS cohort (14,2%), neither of which had a documented infection based on classical bacteriological tests. No fungal infections were detected using the VYOO® technology while microbiology revealed one positive blood culture (C. albicans) and positivity in five other samples (1 peritonitis, 3 lung infections, 1 surgical site). According to microbiology, 48% of the patients were infected with Gram-negative bacteria, among whom 28% were revealed by the VYOO® technology. Among the pathogens revealed by microbiological analysis and the VYOO® technology, E. coli was the most prominent Gram-negative bacteria (34,6% and 19,6% respectively) (figure 2). Among Gram-positive-bacteria the frequency of Staphylococcus aureus (incl. MRSA) was 22.7% according to microbiological analysis and 30.8% according to the VYOO® technology. The correspondence in bacterial identification between microbiology and the VYOO® technology was 46.2% (figure 2).

Bottom Line: Of these, 51.4% were positive using the VYOO® test.The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%.This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

View Article: PubMed Central - PubMed

Affiliation: Unit Cytokines & Inflammation, Department of Infection and Epidemiology, Institut Pasteur, Paris, France.

ABSTRACT
Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOO®) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) for pathogen detection provided by SIRS-Lab (Jena, Germany). We analyzed 72 sepsis patients and 14 non-infectious systemic inflammatory response syndrome (SIRS) patients. Among the sepsis patients, 20 had a positive blood culture and 35 had a positive microbiology in other biological samples. Of these, 51.4% were positive using the VYOO® test. Among the sepsis patients with a negative microbiology and the non-infectious SIRS, 29.4% and 14.2% were positive with the VYOO® test, respectively. The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%. This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

Show MeSH
Related in: MedlinePlus