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DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients.

Fitting C, Parlato M, Adib-Conquy M, Memain N, Philippart F, Misset B, Monchi M, Cavaillon JM, Adrie C - PLoS ONE (2012)

Bottom Line: Of these, 51.4% were positive using the VYOO® test.The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%.This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

View Article: PubMed Central - PubMed

Affiliation: Unit Cytokines & Inflammation, Department of Infection and Epidemiology, Institut Pasteur, Paris, France.

ABSTRACT
Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOO®) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) for pathogen detection provided by SIRS-Lab (Jena, Germany). We analyzed 72 sepsis patients and 14 non-infectious systemic inflammatory response syndrome (SIRS) patients. Among the sepsis patients, 20 had a positive blood culture and 35 had a positive microbiology in other biological samples. Of these, 51.4% were positive using the VYOO® test. Among the sepsis patients with a negative microbiology and the non-infectious SIRS, 29.4% and 14.2% were positive with the VYOO® test, respectively. The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%. This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

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Related in: MedlinePlus

Microbiology analysis in sepsis and SIRS patients.A. Number of sepsis patients with positive blood culture, positive microbiology in other compartments (BAL, urine, catheter, ascitis, peritoneal fluid, synovial fluid, cerebrospinal fluid, bile, skin and bone biopsies), and total number of sepsis patients with a positive VYOO® test. B. Number of non-infectious SIRS patients with a positive VYOO® test. C. Number of sepsis patients with a positive blood culture who had a positive VYOO® test. D. Number of sepsis patients who had a negative blood culture but had a positive microbiology test in other compartments who had a positive VYOO® test. E. Number of sepsis patients without any positive microbiology test who had a positive VYOO® test.
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pone-0038916-g001: Microbiology analysis in sepsis and SIRS patients.A. Number of sepsis patients with positive blood culture, positive microbiology in other compartments (BAL, urine, catheter, ascitis, peritoneal fluid, synovial fluid, cerebrospinal fluid, bile, skin and bone biopsies), and total number of sepsis patients with a positive VYOO® test. B. Number of non-infectious SIRS patients with a positive VYOO® test. C. Number of sepsis patients with a positive blood culture who had a positive VYOO® test. D. Number of sepsis patients who had a negative blood culture but had a positive microbiology test in other compartments who had a positive VYOO® test. E. Number of sepsis patients without any positive microbiology test who had a positive VYOO® test.

Mentions: Twenty sepsis patients had a positive blood culture and 35 had a positive culture in another biological sample (i.e. broncho-alveolar lavages, urine, catheter, ascitis, peritoneal fluid, synovial fluid, cerebrospinal fluid, bile, skin, or bone biopsies) (figure 1). According to blood cultures and cultures of other biological samples, infections were due to Gram-negative bacteria (14 cases), Gram-positive bacteria (20 cases), mixed Gram-negative and Gram-positive bacteria (12 cases), anaerobes (2 cases), and Candida spp (1 case). Five cases were displaying both fungal (4 Candida albicans, 1 C. lusitaniae) and bacterial infection in other compartments thanblood, and 18 had no identified organism.


DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients.

Fitting C, Parlato M, Adib-Conquy M, Memain N, Philippart F, Misset B, Monchi M, Cavaillon JM, Adrie C - PLoS ONE (2012)

Microbiology analysis in sepsis and SIRS patients.A. Number of sepsis patients with positive blood culture, positive microbiology in other compartments (BAL, urine, catheter, ascitis, peritoneal fluid, synovial fluid, cerebrospinal fluid, bile, skin and bone biopsies), and total number of sepsis patients with a positive VYOO® test. B. Number of non-infectious SIRS patients with a positive VYOO® test. C. Number of sepsis patients with a positive blood culture who had a positive VYOO® test. D. Number of sepsis patients who had a negative blood culture but had a positive microbiology test in other compartments who had a positive VYOO® test. E. Number of sepsis patients without any positive microbiology test who had a positive VYOO® test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376137&req=5

pone-0038916-g001: Microbiology analysis in sepsis and SIRS patients.A. Number of sepsis patients with positive blood culture, positive microbiology in other compartments (BAL, urine, catheter, ascitis, peritoneal fluid, synovial fluid, cerebrospinal fluid, bile, skin and bone biopsies), and total number of sepsis patients with a positive VYOO® test. B. Number of non-infectious SIRS patients with a positive VYOO® test. C. Number of sepsis patients with a positive blood culture who had a positive VYOO® test. D. Number of sepsis patients who had a negative blood culture but had a positive microbiology test in other compartments who had a positive VYOO® test. E. Number of sepsis patients without any positive microbiology test who had a positive VYOO® test.
Mentions: Twenty sepsis patients had a positive blood culture and 35 had a positive culture in another biological sample (i.e. broncho-alveolar lavages, urine, catheter, ascitis, peritoneal fluid, synovial fluid, cerebrospinal fluid, bile, skin, or bone biopsies) (figure 1). According to blood cultures and cultures of other biological samples, infections were due to Gram-negative bacteria (14 cases), Gram-positive bacteria (20 cases), mixed Gram-negative and Gram-positive bacteria (12 cases), anaerobes (2 cases), and Candida spp (1 case). Five cases were displaying both fungal (4 Candida albicans, 1 C. lusitaniae) and bacterial infection in other compartments thanblood, and 18 had no identified organism.

Bottom Line: Of these, 51.4% were positive using the VYOO® test.The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%.This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

View Article: PubMed Central - PubMed

Affiliation: Unit Cytokines & Inflammation, Department of Infection and Epidemiology, Institut Pasteur, Paris, France.

ABSTRACT
Fast and reliable assays to precisely define the nature of the infectious agents causing sepsis are eagerly anticipated. New molecular biology techniques are now available to define the presence of bacterial or fungal DNA within the bloodstream of sepsis patients. We have used a new technique (VYOO®) that allows the enrichment of microbial DNA before a multiplex polymerase chain reaction (PCR) for pathogen detection provided by SIRS-Lab (Jena, Germany). We analyzed 72 sepsis patients and 14 non-infectious systemic inflammatory response syndrome (SIRS) patients. Among the sepsis patients, 20 had a positive blood culture and 35 had a positive microbiology in other biological samples. Of these, 51.4% were positive using the VYOO® test. Among the sepsis patients with a negative microbiology and the non-infectious SIRS, 29.4% and 14.2% were positive with the VYOO® test, respectively. The concordance in bacterial identification between microbiology and the VYOO® test was 46.2%. This study demonstrates that these new technologies offer great hopes, but improvements are still needed.

Show MeSH
Related in: MedlinePlus