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Two loci on chromosome 5 are associated with serum IgE levels in Labrador retrievers.

Owczarek-Lipska M, Lauber B, Molitor V, Meury S, Kierczak M, Tengvall K, Webster MT, Jagannathan V, Schlotter Y, Willemse T, Hendricks A, Bergvall K, Hedhammar A, Andersson G, Lindblad-Toh K, Favrot C, Roosje P, Marti E, Leeb T - PLoS ONE (2012)

Bottom Line: We identified a genome-wide significant association on CFA 5 with the antigen-specific IgE responsiveness to Acarus siro.We detected a second genome-wide significant association with respect to the antigen-specific IgE responsiveness to Tyrophagus putrescentiae at a different locus on chromosome 5.No obvious candidate gene for the regulation of IgE levels is located under the two association signals.

View Article: PubMed Central - PubMed

Affiliation: Institute of Genetics, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

ABSTRACT
Crosslinking of immunoglobulin E antibodies (IgE) bound at the surface of mast cells and subsequent mediator release is considered the most important trigger for allergic reactions. Therefore, the genetic control of IgE levels is studied in the context of allergic diseases, such as asthma, atopic rhinitis, or atopic dermatitis (AD). We performed genome-wide association studies in 161 Labrador Retrievers with regard to total and allergen-specific immunoglobulin E (IgE) levels. We identified a genome-wide significant association on CFA 5 with the antigen-specific IgE responsiveness to Acarus siro. We detected a second genome-wide significant association with respect to the antigen-specific IgE responsiveness to Tyrophagus putrescentiae at a different locus on chromosome 5. A. siro and T. putrescentiae both belong to the family Acaridae and represent so-called storage or forage mites. These forage mites are discussed as major allergen sources in canine AD. No obvious candidate gene for the regulation of IgE levels is located under the two association signals. Therefore our studies offer a chance of identifying a novel mechanism controlling the host's IgE response.

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Related in: MedlinePlus

Results of the genome-wide association study restricted to SNP markers located on CFA5.(A) GWAS in the cohort of 161 Labradors showed a significant association of the elevated A. siro specific IgE levels in 136 A. siro IgE-positive dogs and 25 A. siro IgE-negative dogs. (B) Associated SNP markers (black) and minor allele frequency (green) at the locus of the IgE-responsiveness to A. siro zoomed into the interval between 75 Mb and 85 Mb. (C) The gene content of the 3.5-Mb chromosomal interval estimated according to the dog build 2 and displayed using UCSC Genome Browser (http://genome.ucsc.edu).
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pone-0039176-g002: Results of the genome-wide association study restricted to SNP markers located on CFA5.(A) GWAS in the cohort of 161 Labradors showed a significant association of the elevated A. siro specific IgE levels in 136 A. siro IgE-positive dogs and 25 A. siro IgE-negative dogs. (B) Associated SNP markers (black) and minor allele frequency (green) at the locus of the IgE-responsiveness to A. siro zoomed into the interval between 75 Mb and 85 Mb. (C) The gene content of the 3.5-Mb chromosomal interval estimated according to the dog build 2 and displayed using UCSC Genome Browser (http://genome.ucsc.edu).

Mentions: The 27 best associated markers of the GWAS were clustered on CFA 5 in an interval ranging from 79.0–86.7 Mb. Only the 28th best-associated marker resided at a different locus on CFA 37. The observed linkage disequilibrium of the association signals on CFA 5 delineates the associated interval to approximately 3.5 Mb ranging from 79.0–82.5 Mb (Figure 2).


Two loci on chromosome 5 are associated with serum IgE levels in Labrador retrievers.

Owczarek-Lipska M, Lauber B, Molitor V, Meury S, Kierczak M, Tengvall K, Webster MT, Jagannathan V, Schlotter Y, Willemse T, Hendricks A, Bergvall K, Hedhammar A, Andersson G, Lindblad-Toh K, Favrot C, Roosje P, Marti E, Leeb T - PLoS ONE (2012)

Results of the genome-wide association study restricted to SNP markers located on CFA5.(A) GWAS in the cohort of 161 Labradors showed a significant association of the elevated A. siro specific IgE levels in 136 A. siro IgE-positive dogs and 25 A. siro IgE-negative dogs. (B) Associated SNP markers (black) and minor allele frequency (green) at the locus of the IgE-responsiveness to A. siro zoomed into the interval between 75 Mb and 85 Mb. (C) The gene content of the 3.5-Mb chromosomal interval estimated according to the dog build 2 and displayed using UCSC Genome Browser (http://genome.ucsc.edu).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3376118&req=5

pone-0039176-g002: Results of the genome-wide association study restricted to SNP markers located on CFA5.(A) GWAS in the cohort of 161 Labradors showed a significant association of the elevated A. siro specific IgE levels in 136 A. siro IgE-positive dogs and 25 A. siro IgE-negative dogs. (B) Associated SNP markers (black) and minor allele frequency (green) at the locus of the IgE-responsiveness to A. siro zoomed into the interval between 75 Mb and 85 Mb. (C) The gene content of the 3.5-Mb chromosomal interval estimated according to the dog build 2 and displayed using UCSC Genome Browser (http://genome.ucsc.edu).
Mentions: The 27 best associated markers of the GWAS were clustered on CFA 5 in an interval ranging from 79.0–86.7 Mb. Only the 28th best-associated marker resided at a different locus on CFA 37. The observed linkage disequilibrium of the association signals on CFA 5 delineates the associated interval to approximately 3.5 Mb ranging from 79.0–82.5 Mb (Figure 2).

Bottom Line: We identified a genome-wide significant association on CFA 5 with the antigen-specific IgE responsiveness to Acarus siro.We detected a second genome-wide significant association with respect to the antigen-specific IgE responsiveness to Tyrophagus putrescentiae at a different locus on chromosome 5.No obvious candidate gene for the regulation of IgE levels is located under the two association signals.

View Article: PubMed Central - PubMed

Affiliation: Institute of Genetics, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

ABSTRACT
Crosslinking of immunoglobulin E antibodies (IgE) bound at the surface of mast cells and subsequent mediator release is considered the most important trigger for allergic reactions. Therefore, the genetic control of IgE levels is studied in the context of allergic diseases, such as asthma, atopic rhinitis, or atopic dermatitis (AD). We performed genome-wide association studies in 161 Labrador Retrievers with regard to total and allergen-specific immunoglobulin E (IgE) levels. We identified a genome-wide significant association on CFA 5 with the antigen-specific IgE responsiveness to Acarus siro. We detected a second genome-wide significant association with respect to the antigen-specific IgE responsiveness to Tyrophagus putrescentiae at a different locus on chromosome 5. A. siro and T. putrescentiae both belong to the family Acaridae and represent so-called storage or forage mites. These forage mites are discussed as major allergen sources in canine AD. No obvious candidate gene for the regulation of IgE levels is located under the two association signals. Therefore our studies offer a chance of identifying a novel mechanism controlling the host's IgE response.

Show MeSH
Related in: MedlinePlus